Shaffermosegaard2874

By contrast, CA1 mRNA was increased after both rhEPO microdosing and hypoxia, whereas SLC4A1 mRNA did not significantly increase under either condition. Furthermore, the analyses described here were performed using dried blood spots (DBSs), which provide advantages in terms of the sample collection, transport, and storage logistics. This study demonstrates that ALAS2 mRNA levels are sensitive and specific transcriptomic biomarkers for the detection of rhEPO microdosing using the hematological module of the ABP, and this method is compatible with the use of DBSs for anti-doping analyses.Detection of HLA-specific memory B cells can provide additional information on sensitization of alloantigen-exposed individuals and refine immunological risk assessment. We have recently developed an assay enabling profiling of memory B cell-derived HLA antibodies using luminex single antigen bead (SAB) assay. Here, we compared the performance of the SAB kits from two vendors for memory B cell-derived HLA antibody detection. IgG was isolated from culture supernatants of polyclonally activated B cells from alloantigen-exposed (n = 7) or nonexposed (n = 5) individuals, using our previously established method. Eluates containing isolated IgG from culture supernatants were tested for the presence of HLA antibodies using luminex SAB analysis from both One Lambda and Lifecodes (Immucor). In contrast to Lifecodes, high mean fluorescence intensity (MFI) signals were found for negative control beads in One Lambda (median MFI for class I1730 and for class II728), accompanied by high MFI values for self HLA-coated beads, especially for HLA-C. Despite high background in the One Lambda assays, 91% concordance for HLA class I and 85% concordance for HLA class II were found between the specificities detected using SAB kits from the two vendors. Our results show that HLA-specific memory B cells can be profiled using kits from both vendors. However, when analyzing One Lambda results one should be aware of the restrictions related to nonspecific binding particularly in HLA-C-coated beads, and pay attention to self HLA-coated beads in order to accurately identify the reactivities leading to the definition of the actual HLA antibody specificities.Pancreatic islet cell transplantation has proven efficacy as a treatment for type 1 diabetes mellitus, chiefly in individuals who are refractory to conventional insulin replacement therapy. At present its clinical use is restricted, firstly by the limited access to suitable donor organs but also due to factors associated with the current clinical transplant procedure which inadvertently impair the long-term functionality of the islet graft. Of note, the physical, biochemical, inflammatory, and immunological stresses to which islets are subjected, either during pretransplant processing or following implantation are detrimental to their sustained viability, necessitating repeated islet infusions to attain adequate glucose control. Progressive decline in functional beta (β)-cell mass leads to graft failure and the eventual re-instatement of exogenous insulin treatment. Strategies which protect and/or preserve optimal islet function in the peri-transplant period would improve clinical outcomes. Human amniotic epithelial cells (HAEC) exhibit both pluripotency and immune-privilege and are ideally suited for use in replacement and regenerative therapies. The HAEC secretome exhibits trophic, anti-inflammatory, and immunomodulatory properties of relevance to islet graft survival. Facilitated by β-cell supportive 3D cell culture systems, HAEC may be integrated with islets bringing them into close spatial arrangement where they may exert paracrine influences that support β-cell function, reduce hypoxia-induced islet injury, and alter islet alloreactivity. The present review details the potential of multifunctional HAEC in the context of islet transplantation, with a focus on the innate capabilities that may counter adverse events associated with the current clinical transplant protocol to achieve long-term islet graft function.Transition metal oxides (TMOs) have been under the spotlight as promising precatalysts for electrochemical oxygen evolution reaction (OER) in alkaline media. However, the slow and incomplete self-reconstruction from TMOs to (oxy)hydroxides as well as the formed (oxy)hydroxides with unmodified electronic structure gives rise to the inferior OER performance to the noble metal oxide ones. Herein, a unique dual metal oxides lattice coupling strategy is proposed to fabricate carbon cloth-supported ultrathin nanowires arrays, which are composed of pseudo-periodically welded NiO with CeO2 nanocrystals (NiO/CeO2 NW@CC). When served as an OER precatalyst in 1.0 m KOH, the NiO/CeO2 NW@CC shows an ultralow overpotential of 330 mV at 50 mA cm-2 , along with an impressive cycle durability of more than 3 days even at 50 mA cm-2 , surpassing CC-supported NiO and commercial IrO2 catalysts. The combined experimental and theoretical investigations unveil that the atomic coupling of CeO2 can not only appreciably trigger the generation of oxygen vacancies and expedite phase transformation of NiO into active NiOOH, but also in situ create a chemical bond with the formed NiOOH and enable the electron injection, thus effectively inhibiting the aggregation of the accessible NiOOH nanodomains and optimizing their reaction free energy towards oxygen-containing intermediates.Cell-laden microgels have attracted increasing interest in various biomedical fields, as living building blocks to construct spatially organized multicellular structures or complex tissue features (e.g., cell spheroids and aligned cells/fibers). Although numerous approaches have been developed to tailor cell-laden microgels, there is still an unmet need for modular, versatile, convenient, and high-throughput methods. In this study, as inspired by the phenomena of water droplet manipulation from natural microstructures, a novel platform is developed to manipulate microscale hydrogel droplets and fabricate modular cell-laden microgels. First, taking antenna-like trichome as a template, catcher-like bioinspired microstructures are fabricated and hydrogel droplets are manipulated modularly in a versatile, convenient, and high-throughput manner, which is compatible with various types of hydrogels (e.g., photo-cross-linking, thermal-cross-linking, and ion-cross-linking). It is demonstrated that this platform can manipulate cell-laden microgels as modular units, such as two or more cell-laden microgels on one single catcher-like structure and different structures on one single chip. The authors also demonstrate the application of this platform on constructing complex tissue features like myocardial fibrosis tissue models to study cardiac fibrosis. The developed platform will be a powerful tool for engineering various in vitro tissue models for widespread biomedical applications.Magnesium metal batteries (MMBs) have obtained the reputation owing to the high volumetric capacity, low reduction potential, and dendrite-free deposition behavior of the Mg metal anode. However, the bivalent nature of the Mg2+ causes its strong coulombic interaction with the cathode host, which limits the reaction kinetics and reversibility of MMBs, especially based on oxide cathodes. Herein, a synergetic modulation of host pillaring and electrolyte formulation is proposed to activate the layered V2 O5 cathode with expanded interlayers via sequential intercalations of poly(3,4-ethylenedioxythiophene) (PEDOT) and cetyltrimethylammonium bromide (CTAB). The preservation of bundled nanowire texture, copillaring behavior of PEDOT and CTA+ , dual-insertion mode of Mg2+ and MgCl+ at cathode side enable the better charge transfers in both the bulk and interface paths as well as the interaction mitigation effect between Mg-species cations and host lattices. The introduction of CTA+ as electrolyte additive can also lower the interface resistance and smoothen the Mg anode morphology. These modifications endow the full cells coupled with metallic Mg anode with the maximized reversible capacity (288.7 mAh g-1 ) and superior cyclability (over 500 cycles at 500 mA g-1 ), superior to most already reported Mg-ion shuttle batteries even based on passivation-resistant non-Mg anodes or operated at higher temperatures.Graphene is extensively investigated for various energy storage systems. However, the very low density ( less then 0.01 g cm-3 ) of graphene nanosheets has hindered its further applications. To solve this issue, a controlled assembly of 2D graphene building blocks should be developed into graphene microspheres with high packing density, and restacking of graphene should be prevented to ensure an electrochemically accessible surface area during the assembly. Furthermore, graphene microspheres should have multiple 1D external conductive architecture to promote contacts with the neighbors. This study reports in situ growth of novel graphene nanostructures in reduced graphene oxide microspherical assembly (denoted as GT/GnS@rGB) with restacking resistance and interparticle contacts, for electrochemical energy storage. The GT/GnS@rGB showed high gravimetric (231.8 F g-1 ) and volumetric (181.5 F cm-3 ) capacitances at 0.2 A g-1 in organic electrolyte with excellent rate capabilities of 94.3% (@ 0.2 vs 10 Ag-1 ). Furthermore, GT/GnS@rGB exhibited excellent cycling stability (96.1% of the initial capacitance after 100 000 charge/discharge cycles at 2 A g-1 ). As demonstrated in the electrochemical evaluation as electrode materials for electrical double-layer capacitors, unique structural and textural features of the GT/GnS@rGB would be beneficial in the use of graphene assembly for energy storage applications.Severe cardiac damage following myocardial infarction (MI) causes excessive inflammation, which sustains tissue damage and often induces adverse cardiac remodeling toward cardiac function impairment and heart failure. Timely resolution of post-MI inflammation may prevent cardiac remodeling and development of heart failure. Cell therapy approaches for MI are time-consuming and costly, and have shown marginal efficacy in clinical trials. Here, nanoparticles targeting the immune system to attenuate excessive inflammation in infarcted myocardium are presented. Liposomal nanoparticles loaded with MI antigens and rapamycin (L-Ag/R) enable effective induction of tolerogenic dendritic cells presenting the antigens and subsequent induction of antigen-specific regulatory T cells (Tregs). find more Impressively, intradermal injection of L-Ag/R into acute MI mice attenuates inflammation in the myocardium by inducing Tregs and an inflammatory-to-reparative macrophage polarization, inhibits adverse cardiac remodeling, and improves cardiac function. Nanoparticle-mediated blocking of excessive inflammation in infarcted myocardium may be an effective intervention to prevent the development of post-MI heart failure.2D non-layered materials (2DNLMs) featuring massive undercoordinated surface atoms and obvious lattice distortion have shown great promise in catalytic/electrocatalytic applications, but their controllable synthesis remains challenging. Here, a new type of ultrathin carbon-wrapped titanium nitride nanomesh (TiN NM@C) is prepared using a rationally designed nano-confinement topochemical conversion strategy. The ultrathin 2D geometry with well-distributed pores offers TiN NM@C plentiful exposed active sites and rapid charge transfer, leading to outstanding electrocatalytic performance tackling the sluggish sulfur redox kinetics in lithium-sulfur batteries (LSBs). LSBs employing TiN NM@C electrocatalyst deliver excellent rate capabilities (e.g., 304 mAh g-1 at 10 C), greatly outperforming that of using TiN nanoparticles embedded in carbon nanosheets (TiN NPs@C) as a benchmark. More impressively, a free-standing electrode for LSBs with a high sulfur loading of 7.3 mg cm-2 is demonstrated, showing a high peak areal capacity of 5.