Thomasbrogaard2151

Water steam distillation is a classical method of rose oil production from the flowers of Rosa damascena Mill. This process produces considerable amount of waste water. In this study, ion-exchange column chromatography (Amberlite was the stationary phase) was used to prepare polyphenol-enriched fraction of waste water with improved biological activity. Phenol, flavonoid, and anthocyanin contents were examined before and after using column. Antioxidant activities, DNA protection ability, xanthine oxidase inhibition, and cytotoxicity of this fraction were also determined. The use of Amberlite increased phenol, flavonoid, and anthocyanin contents in fraction compared to the sample before fractionation. The IC50 values of various antioxidant assays comprises 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power assay (FRAP), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) which were 226.66 ± 1.25, 126.03 ± 0.11, and 241.43 ± 0.33 for waste water, and these values for fraction were 63.21 ± 0.90, 34.6 ± 0.17, and 50.59 ± 0.75 μg/ml, respectively. The Trolox equivalent values of fraction in oxygen radical absorbance capacity (ORAC) assay were 0.34 ± 0.04, and the EC50 values in cellular antioxidant activity were 91.24 ± 0.32 μg/ml. The xanthine oxidase inhibition capacity of fraction (100 μg/ml) was 96.4 ± 0.02% μg/ml. The comet assay analysis showed that this fraction (25-100 μg/ml) protects human lymphocytes against H2O2-induced DNA damages significantly. The IC50 values of cytotoxicity assay were 248.145 ± 35.56 and 227.14 ± 16.51 μg/ml after 24 and 48 h, respectively. There has been great attention to the valorization of waste materials. Recovered fraction could be considered as a proper antioxidant, DNA damage-protection agent, and xanthine oxidase inhibitor. Using a nontoxic solid phase such as Amberlite is a fruitful way to concentrate bioactive ingredients which can be used in pharmaceutical and nutraceutical industry.Cluster analysis is a fundamental tool for pattern discovery of complex heterogeneous data. Prevalent clustering methods mainly focus on vector or matrix-variate data and are not applicable to general-order tensors, which arise frequently in modern scientific and business applications. Moreover, there is a gap between statistical guarantees and computational efficiency for existing tensor clustering solutions due to the nature of their non-convex formulations. Akt inhibitor In this work, we bridge this gap by developing a provable convex formulation of tensor co-clustering. Our convex co-clustering (CoCo) estimator enjoys stability guarantees and its computational and storage costs are polynomial in the size of the data. We further establish a non-asymptotic error bound for the CoCo estimator, which reveals a surprising "blessing of dimensionality" phenomenon that does not exist in vector or matrix-variate cluster analysis. Our theoretical findings are supported by extensive simulated studies. Finally, we apply the CoCo estimator to the cluster analysis of advertisement click tensor data from a major online company. Our clustering results provide meaningful business insights to improve advertising effectiveness.This study explored the relations between Latino gender role attitudes (traditional machismo attitudes and caballerismo attitudes) and sexual behaviors among 242 Mexican American early adolescent boys in the southwest United States. Specifically, a multiple mediator model estimated the association between gender role attitudes and sexual activity through a mediational pathway connecting substance use, sexual motives, and peer influence. Results from analyzing this structural equation model indicated that traditional machismo attitudes were not associated with sexual behaviors. Caballerismo gender role attitudes, however, were indirectly linked with reduced sexual motives and substance via peer influence. This study underscores the importance of social context, behavior, and psychological motivations in explaining differences in sexual motives and substance use among young Mexican American adolescent boys who hold different kinds of beliefs about their gender.Causal mediation analysis provides investigators insight into how a treatment or exposure can affect an outcome of interest through one or more mediators on causal pathway. When multiple mediators on the pathway are causally ordered, identification of mediation effects on certain causal pathways requires a sensitivity parameter to be specified. A mixed model-based approach was proposed in the Bayesian framework to connect potential outcomes at different treatment levels, and identify mediation effects independent of a sensitivity parameter, for the natural direct and indirect effects on all causal pathways. The proposed method is illustrated in a linear setting for mediators and outcome, with mediator-treatment interactions. Sensitivity analysis was performed for the prior choices in the Bayesian models. The proposed Bayesian method was applied to an adolescent dental health study, to see how social economic status can affect dental caries through a sequence of causally ordered mediators in dental visit and oral hygiene index.High-fructose diet induced changes in gut microbiota structure and function, which have been linked to inflammatory response. However, the effect of small or appropriate doses of fructose on gut microbiota and inflammatory cytokines is not fully understood. Hence, the abundance changes of gut microbiota in fructose-treated Sprague-Dawley rats were analyzed by 16S rRNA sequencing. The effects of fructose diet on metabolic disorders were evaluated by blood biochemical parameter test, histological analysis, short-chain fatty acid (SCFA) analysis, ELISA analysis, and Western blot. Rats were intragastrically administered with pure fructose at the dose of 0 (Con), 2.6 (Fru-L), 5.3 (Fru-M), and 10.5 g/kg/day (Fru-H) for 20 weeks. The results showed that there were 36.5% increase of uric acid level in the Fru-H group when compared with the Con group. The serum proinflammatory cytokines (IL-6, TNF-α, and MIP-2) were significantly increased (P less then 0.05), and the anti-inflammatory cytokine IL-10 was significantly decreased (P less then 0.05) with fructose treatment. A higher fructose intake induced lipid accumulation in the liver and inflammatory cell infiltration in the pancreas and colon and increased the abundances of Lachnospira, Parasutterella, Marvinbryantia, and Blantia in colonic contents. Fructose intake increased the expressions of lipid accumulation proteins including perilipin-1, ADRP, and Tip-47 in the colon. Moreover, the higher level intake of fructose impaired intestinal barrier function due to the decrease of the expression of tight junction proteins (ZO-1 and occludin). In summary, there were no negative effects on body weight, fasting blood glucose, gut microbiota, and SCFAs in colonic contents of rats when fructose intake is in small or appropriate doses. High intake of fructose can increase uric acid, proinflammatory cytokines, intestinal permeability, and lipid accumulation in the liver and induce inflammatory response in the pancreas and colon.Peri-implant osteolysis (PIO) and the subsequent aseptic loosening are the main reasons for artificial joint implant failure. Existing methods for treating aseptic loosening are far from satisfactory, necessitating advanced drug exploration. This study is aimed at investigating the effect and underlying mechanism of tetrandrine (Tet) on inflammatory osteolysis. We established a Ti particle-induced inflammatory osteolysis mouse model and administered Tet or an equal volume of phosphate-buffered saline (PBS). Two weeks later, specimens were collected. Histological staining showed that Tet administration inhibited Ti-stimulated osteolysis. Tartrate-resistant acid phosphate (TRAP) staining and transmission electron microscopy (TEM) demonstrated that osteoclast formation was remarkably inhibited in the groups treated with Tet in a dose-dependent manner. In addition, relevant inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6) were also significantly reduced in the calvaria of the Tet-treated groups. Exposure of receptor activator for nuclear factor-κB ligand- (RANKL-) induced bone marrow-derived macrophages (BMMs) and RAW264.7 cells to Tet significantly reduced osteoclast formation, F-actin ring formation, bone resorption, and the expression of relevant genes (matrix metallopeptidase 9 (MMP-9), TRAP, and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1)) during osteoclastogenesis in vitro. Mechanistic studies using Western blotting demonstrated that Tet inhibited the nuclear factor (NF)-κB signaling pathway by decreasing the phosphorylation of inhibitor of NF-κB α (IκBα) and p65, which play important roles in osteoclast formation. Collectively, our data indicate that Tet suppressed Ti-induced inflammatory osteolysis and osteoclast formation in mice, suggesting that Tet has the potential to be developed to treat and prevent wear particle-induced inflammatory osteolysis.With legal animal rights on the horizon, there is a need for a more systematic theorisation of animal rights as legal rights. This article addresses conceptual, doctrinal and normative issues relating to the nature and foundations of legal animal rights by examining three key questions can, do and should animals have legal rights? It will show that animals are conceptually possible candidates for rights ascriptions. Moreover, certain 'animal welfare rights' could arguably be extracted from existing animal welfare laws, even though these are currently imperfect and weak legal rights at best. Finally, this article introduces the new conceptual vocabulary of simple and fundamental animal rights, in order to distinguish the weak legal rights that animals may be said to have as a matter of positive law from the kind of strong legal rights that animals ought to have as a matter of future law.

Data for predicting severity of patients with COVID-19 infection are sparse and still under investigation. We retrospectively studied whether the admission serum C-reactive protein level (CRP) can serve as nearly predictor of disease severity during COVID-19 infection in comparison with other hematologic and inflammatory markers.

We included all consecutive patients who were admitted in Cheikh Khalifa International University Hospital, Casablanca, Morocco, between February to April 2020, with a confirmed diagnosis of COVID-19 infection using SARS-CoV-2 viral nucleic acid via RT-PCR. The complete blood count and serum CRP level were routinely measured on admission. All clinical and laboratory data of patients were collected and analyzed. The classification of the disease severity was in accordance with the clinical classification of the WHO interim guidance, and the management of patients were adapted to the national management guideline. We estimated receiver operating characteristic (ROC) curves of bloode of primary care.

This study found that the CRP level at admission represent a simple and independent factor that can be useful for early detection of severity during COVID-19 and the easy guidance of primary care.