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Western blot and immunohistochemistry confirmed this upregulation, with an up to 20-fold increase in ICAM-1 signal. Radiolabelled anti-ICAM-1 bound to ICAM-1 expressing cells with good affinity (Kd = 24.0 ± 4.0 nM). [111In]In-anti-ICAM-1 uptake in tumours at 72 h post injection was approximately 3-fold higher than non-specific isotype-matched [111In]In-mIgG2a control (19.3 ± 2.5%ID/g versus 6.3 ± 2.2%ID/g, P = 0.0002). However, ICAM1 levels, and [111In]In-anti-ICAM-1 uptake in tumours was no different after irradiation (uptake 9.2%ID/g versus 14.8%ID/g). Western blots of the xenograft lysates showed no significant differences, confirming these results. CONCLUSION Imaging of ICAM-1 is feasible in mouse models of pancreatic cancer. Although ICAM-1 is upregulated post-irradiation in in vitro models of pancreatic cancer, it shows little change in expression in an in vivo mouse xenograft model. INTRODUCTION Pretargeting strategies that do not rely on the expression of molecular targets have expanded imaging and therapy options for cancer patients. Nanostars with designed multivalency and which highly accumulate in tumor tissue via the enhanced permeability and retention (EPR) effect may therefore be the ideal vectors for the development of a passive pretargeting approach. METHODS Nanostars were synthesized, consisting of 7-8 center-cross-linked arms that were modified with trans-cyclooctene (TCO) using poly(ethylene glycol) (PEG) linkers of 12 or 106 monomer units or without linker. The bioorthogonal click reaction with radiofluorinated 2,2'-(7-(2-(tetrazine-poly(ethyleneglycol)11-amino)-2-oxoethyl)-1,4,7-triazonane-1,4-diyl)diacetic acid ([18F]F-Tz-PEG11-NODA) or 2,2'-(7-(2-(tetrazine-amino)-2-oxoethyl)-1,4,7-triazonane-1,4-diyl)diacetic acid ([18F]F-Tz-NODA) was measured by ex vivo biodistribution studies and positron emission tomography (PET) in mice bearing tumors with high EPR characteristics. Bioorthogonal masking was performed using a tetrazine-functionalized dextran polymer (Tz-DP). RESULTS Highest tumor accumulation of [18F]F-Tz-PEG11-NODA was observed for nanostars functionalized with TCO without linker, with a tumor uptake of 3.2 ± 0.4%ID/g and a tumor-to-muscle ratio of 12.8 ± 4.2, tumor-to-large intestine ratio of 0.5 ± 0.3 and tumor-to-kidney ratio of 2.0 ± 0.3, being significantly higher than for nanostars functionalized with TCO-PEG12 (P  less then  0.05) or TCO-PEG106 (P  less then  0.05). Tumor uptake and tumor-to-tissue ratios did not improve upon bioorthogonal masking with Tz-DP or when using a smaller, more lipophilic tetrazine([18F]F-Tz-NODA). CONCLUSIONS A pretargeting strategy was developed based on the passive delivery of TCO-functionalized nanostars. Such a strategy would allow for the imaging and treatment of tumors with apparent EPR characteristics, with high radioactive tumor doses and minimal doses to off-target tissues. Bioelectrochemical systems like microbial fuel cells (MFCs) are quaint systems known to metamorphose the chemical energy of organic matter into electrical energy using catalytic activity of microorganisms. A novel continuous Auto Circulating Bio-Electrochemical Reactor (AutoCirBER) was developed to fulfil the gap of 'simple, inexpensive and compact design' that can continuously treat larger amount of organic wastewater at shorter residence time and without consuming external energy for liquid mixing. AutoCirBER eliminated the need for external agitation for liquid-mixing and therefore, energy requirements. AutoCirBER was operated in continuous-mode and hydraulic retention time was optimized. The reactor underwent performance check-up viz. COD removal, net power output, columbic efficiency, sludge generation and an attributional life cycle assessment (LCA) was also conducted. AutoCirBER was sustainable to run in continuous-mode and showed more than 90.4% of COD removal, and 59.55 W.h net annual energy recovery. Experimental LCA of AutoCirBER also displays its environmental feasibility in longer run. AUPM170 BACKGROUND Overcorrection is a recognized problem following surgical treatment of congenital clubfoot. Recently this complication has also been mentioned following Ponseti treatment. RESEARCH QUESTION Do overcorrected clubfeet (OCCF) caused by surgery behave differently from those caused by Ponseti treatment in terms of segmental motion of the feet and show differences in the severity of deformity on X-rays? METHODS Children between 7 and 12 years with OCCF were included in this study. Depending on the aetiology causing them, the feet were divided into 2 groups (Ponseti and peritalar release surgery). 25 typically developing children served as controls. All subjects were subjected to clinical and radiological examination and 3-Dimensional gait analysis using the Oxford Foot Model. RESULTS Thirty-two children with OCCF, of these 18 feet in the surgical and 14 feet in the Ponseti group, were included in the study. No radiological differences were seen in the flatfoot parameters between OCCF groups except in the calcaneal inclination angle that was more pathological in the Ponseti group. The clinical ankle plantar flexion was significantly reduced in the surgical group. During walking the range motion of the hindfoot in the frontal plane was significantly reduced in surgically treated feet compared to the Ponseti group. The other parameters did not show any significant difference between groups. SIGNIFICANCE The overcorrected clubfeet following surgery and Ponseti showed similar appearance and showed no significant differences in 11/12 radiological parameters. The segmental motion of the feet showed no significant differences between groups except the in the range of motion of the subtalar eversion. A considerable subtalar joint motion was present even in the surgical group. These findings might help plan the treatment of these feet. The enormous attraction on CO2 biofixation using photosynthetic microorganisms such as cyanobacteria has been risen due to its promising efficiency and valuable by-products production. In this study, an isolated cyanobacterium from hot spring in Taiwan, Thermosynechococcus sp. CL-1 (TCL-1) was evaluated for its growth factors arrangement effect on the biomass productivity and CO2 biofixation. The initial biomass concentration, and nutrient supply level variation influenced TCL-1 biomass productivity and CO2 biofixation rate while the adjusted and controlled pH value gave an insignificant difference on its performance. The initial biomass concentration of 3 g L-1 gave the best result on biomass productivity and CO2 fixation which reached 143.4 mg L-1 h-1 and 224 mg L-1 h-1 respectively. Regarding to the result of this study, controlled pH value by the CO2 supply inside the reactor, produced an insignificant difference in TCL-1 performance compared to those with the uncontrolled pH value. The variation of nutrient supply level was achieved by the variation of macronutrient and micronutrient supply inside the medium.