Kelleyjacobsen4583

le-bundle ACL reconstruction, the AM drilling technique was superior to the TT drilling technique based on physical examination, scoring systems, and radiographic results. The AM portal technique provided a more reproducible anatomic graft placement compared with the TT technique.

Technological innovation is a key component of orthopaedic surgery. With the integration of powerful technologies in surgery and clinical practice, artificial intelligence (AI) may become an important tool for orthopaedic surgeons in the future. Through adaptive learning and problem solving that serve to constantly increase accuracy, machine learning algorithms show great promise in orthopaedics.

To investigate the current and potential uses of AI in the management of anterior cruciate ligament (ACL) injury.

Systematic review; Level of evidence, 3.

A systematic review of the PubMed, MEDLINE, Embase, Web of Science, and SPORTDiscus databases between their start and August 12, 2020, was performed by 2 independent reviewers. Inclusion criteria included application of AI anywhere along the spectrum of predicting, diagnosing, and managing ACL injuries. Exclusion criteria included non-English publications, conference abstracts, review articles, and meta-analyses. Statistical analysis could not be performed re is an increasing interest in AI among orthopaedic surgeons, as reflected by the applications for ACL injury presented in this review. Although some studies showed similar or better outcomes using AI compared with traditional techniques, many challenges need to be addressed before this technology is ready for widespread use.

Platelet-rich plasma (PRP) has gained attention as a therapeutic option for knee osteoarthritis; however, its efficacy varies widely. Leukocytes in PRP raise the concern of aggravating proinflammatory activity. To date, PRP has rarely been investigated with regard to leukocyte concentration.

To provide clinical evidence of the intra-articular injection of PRPs containing different leukocyte concentrations.

Systematic review; Level of evidence, 4.

We systematically searched the MEDLINE, Embase, Cochrane Library, CINAHL, and Scopus databases. PRP was classified into leukocyte-poor (LP-PRP) and leukocyte-rich (LR-PRP). Clinical outcomes including Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), visual analog scale (VAS) for pain score, International Knee Documentation Committee (IKDC) subjective score, and adverse reactions were evaluated. The Methodological Index for Non-Randomized Studies criteria were used for quality assessment.

Included were 32 studies with an evidence levelr LR-PRP compared with LP-PRP injection. The findings of this review can support the potential use of intra-articular PRP injection for the treatment of knee osteoarthritis. In clinical application, clinicians need to consider selecting a specific type of PRP for knee osteoarthritis.

Intra-articular PRP injection resulted in improvements above the MCID in terms of pain and function in patients with knee osteoarthritis up to 12 months. The risk of local adverse reactions appeared to be increased after LR-PRP compared with LP-PRP injection. The findings of this review can support the potential use of intra-articular PRP injection for the treatment of knee osteoarthritis. Inflammation antagonist In clinical application, clinicians need to consider selecting a specific type of PRP for knee osteoarthritis.

We sought to determine whether electrical patterns in endocardial wavefronts contained elements specific to atrial fibrillation (AF) disease progression.

A canine paced model (n=7, female mongrel, 29±2 kg) of persistent AF was endocardially mapped with a 64-electrode basket catheter during periods of AF at 1 month, 3 month, and 6 months post-implant of stimulator. A 50-layer residual network was then trained to map half-second electrogram samples to their source timepoint.

The trained network achieved final validation and testing accuracies of 51.6 and 48.5% respectively. Per class F1 scores were 24%, 59%, and 53% for 1 month, 3 month, and 6 month inputs from the testing dataset.

Differentiation of AF based on its time progression was shown to be feasible with a deep learning method. This is promising for differentiating treatment based on disease progression though low accuracy with earlier timepoints may be an obstacle to identifying nascent AF.

Differentiation of AF based on its time progression was shown to be feasible with a deep learning method. This is promising for differentiating treatment based on disease progression though low accuracy with earlier timepoints may be an obstacle to identifying nascent AF.

is a sexually transmitted protozoan parasite and the causative agent of trichomoniasis. The genetic characterization of

isolates shows notable genetic variation in this parasite. In the present study, we aimed to identify the

genotypes based on analyzing of actin gene in women specimens referred to health centers of Ilam city, southwest Iran.

A total of 1765 female samples were collected from gynecology clinics in the city of Ilam. DNA was extracted from positive samples and nested polymerase chain reaction (Nested PCR) was used to amplify the actin gene. Then, partial sequencing and genotyping of the actin gene was performed. A phylogenetic tree was drawn using the detected genotypes of T. vaginalis and reference sequences.

Twenty-one of the 1765 urine and vaginal samples were positive for

All infected individuals were married and their age in years was between 25 to 34. Further, the majority of infected women had cervical lesions, patchy erythema, and white color discharge. According to sequencing analysis, the isolates were identified as genotype G (n= 8) and genotype E (n= 2).

From the collected samples, we were able to distinguish at least two genotypes (G and E) of

. However, lesser is known about these genotypes in the city of Ilam. Further studies with a higher number of isolates should be performed in order to understand the implications of these results in this region.

From the collected samples, we were able to distinguish at least two genotypes (G and E) of T. vaginalis. However, lesser is known about these genotypes in the city of Ilam. Further studies with a higher number of isolates should be performed in order to understand the implications of these results in this region.

High prevalence of oral squamous cell carcinoma (OSCC) demands the additional novel biological markers. Due to the established roles of cytokeratin in the prognosis of metastasis evaluation the relation of expression of both

and

in OSCC compared to the dysplastic oral epithelium biopsies with clinicopathological factors were investigated.

We examined the coordinate mRNA expression of cytokeratin 7 (

) and cytokeratin 20 (

) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 110 biopsies of oral squamous epithelium samples including 72 tumoral and 38 dysplastic biopsies. We also collected demographic and pathological data including tumor stage and grade from our patients.

There was a significant difference in

and

gene expression between OSCC and dysplastic samples (p< 0.001). Further, their mean expression in OSCC samples was significantly higher compared to dysplastic samples. Relative mRNA levels of

and

showed that their mean expression in OSCC grade I was significantly lower than other grades (p< 0.01). The relationship between

and

mRNA expression and age or gender was not significant (p> 0.05). Samples in the advanced stage of disease had significantly higher

and

expression compared to early-stage samples of OSCC specimens (p= 0.001).

We found an increase in

and

mRNA levels in grade III OSCC samples compared to other grades. This finding suggests a potential role for

and

in oral mucosal carcinogenesis and OSCC prognosis.

We found an increase in CK7 and CK20 mRNA levels in grade III OSCC samples compared to other grades. This finding suggests a potential role for CK7 and CK20 in oral mucosal carcinogenesis and OSCC prognosis.

Obesity is a multifactorial metabolic disease resulting from behavioral and genetic factors. Obesity is linked to diabetes mellitus and hypertension, which are considered as major risk factors for chronic kidney disease (CKD); moreover, it has a direct effect on developing CKD and end stage renal disease (ESRD). Here was aimed to examine the association between uncoupling protein 2 (UCP2) gene expression and obesity in CKD patients.

UCP2 gene expression was analyzed by real time polymerase chain reaction (RT-PCR) in 93 participants divided into three groups. The groups included 31 non-obese CKD patients, 31 obese CKD patients, and 31 healthy, age-matched, unrelated volunteers as a control group.

UCP2 gene expression was significantly relevant when comparing the non-obese CKD and obese CKD groups to the control group (p< 0.001). No significant association was found when the groups were compared by gender; Chi-square (X2) was 2.38 and p= 0.304. A significant negative correlation was found between UCP2 gene expression and BMI in CKD (p< 0.05).

These results indicate that UCP2 gene expression plays a significant role as a risk factor for obesity in CKD patients.

These results indicate that UCP2 gene expression plays a significant role as a risk factor for obesity in CKD patients.

Stem cell differentiation therapy is a promising strategy in cancer treatment. we show that protein cocktail prepared from serum starved fibroblasts has therapeutic potential based on this strategy.

The condition medium was prepared from foreskin isolated fibroblasts and analyzed by Liquid chromatography electrospray ionization mass spectrometry-mass spectrometry (LC-ESI-MS/MS). LA7 mammary gland cancer stem cells originated tumors were induced in Sprague Dawley rats. The rats treated subcutaneously with DMEM (group A), condition medium (group B), or normal saline (group C) once daily for 7 days. Then the tumors were removed and divided into the two parts, one part was used to quantify gene expression by stem-loop RT-qPCR assay and the other part was used for Hematoxylin & Eosin (H & E), Giemsa, and immunohistochemistry (IHC) staining.

All induced tumors appeared as sarcomatoid carcinoma (SC). Immunohistochemistry staining confirmed this conclusion by recognizing the tumor as Ki67

, cytokeratin

, vimentine

, and estrogen receptor negative SC. RT-qPCR analysis revealed that

- gene expression was much reduced in the condition medium treated tumors versus proper controls (p< 0.05). Tissue necrosis was more prevalent in this group while tumors volume was diminished almost by 40%. The LC-ESI-MS/MS analysis unrevealed the stemness reducing and the cell death inducing proteins such as, pigment epithelium-derived factor (PEDF), insulin like growth factor binding protein-5 (IGFBP-5) and -7 (IGFBP-7) in the condition medium.

This study showed that the substances released from starved human fibroblasts were able to down-regulate the stemness-related genes and induce necrosis in LA7 derived tumors.

This study showed that the substances released from starved human fibroblasts were able to down-regulate the stemness-related genes and induce necrosis in LA7 derived tumors.