Jensenskipper1807

Histological evaluation plays a major role in cancer diagnosis and treatment. The appearance of H&E-stained images can vary significantly as a consequence of differences in several factors, such as reagents, staining conditions, preparation procedure and image acquisition system. Such potential sources of noise can all have negative effects on computer-assisted classification. To minimize CP-91149 in vitro and their potentially negative effects several color pre-processing methods have been proposed in the literature-for instance, color augmentation, color constancy, color deconvolution and color transfer. Still, little work has been done to investigate the efficacy of these methods on a quantitative basis. #link# In this paper, we evaluated the effects of color constancy, deconvolution and transfer on automated classification of H&E-stained images representing different types of cancers-specifically breast, prostate, colorectal cancer and malignant lymphoma. Our results indicate that in most cases color pre-processing does not improve the classification accuracy, especially when coupled with color-based image descriptors. Some pre-processing methods, however, can be beneficial when used with some texture-based methods like Gabor filters and Local Binary Patterns.The aim of this comparative study is to analyze the influence of drilling technique on the radiographic, thermographic, and geomorphometric effects of dental implant drills and osteotomy site preparations. One hundred and twenty osteotomy site preparations were performed on sixty epoxy resin samples using three unused dental implant drill systems and four drilling techniques performed with a random distribution into the following study groups Group A drilling technique performed at 800 rpm with irrigation (n = 30); Group B drilling technique performed at 45 rpm without irrigation (n = 30); Group C drilling technique performed at 45 rpm with irrigation (n = 30); and Group D drilling technique performed at 800 rpm without irrigation (n = 30). The osteotomy site preparation morphologies performed by the 4.1 mm diameter dental implant drills from each study group were analyzed and compared using a cone beam computed tomography (CBCT) scan. The termographic effects generated by the 4.1 mm diameter dental implant drills from each study group were registered using a termographic digital camera and the unused and 4.1 mm diameter dental implant drills that were used 30 times from each study group were exposed to a micro computed tomography (micro-CT) analysis to obtain a Standard Tessellation Language (STL) digital files that determined the wear comparison by geomorphometry. Statistically significant differences were observed between the thermographic and radiographic results of the study groups (p less then 0.001). The effect of cooling significatively reduced the heat generation during osteotomy site preparation during high-speed drilling; furthermore, osteotomy site preparation was not affected by the wear of the dental implant drills after 30 uses, regardless of the drilling technique.Since the discovery of biological antifreeze glycoproteins (AFGPs), which can inhibit ice nucleation, there has been considerable interest in understanding their mechanisms and mimicking them in synthetic polymers. In this study, we used molecular dynamics simulations of modified polyvinyl alcohol (PVA) compounds to show that the hydroxyl (OH) group distance is a key factor in whether certain compounds promote or inhibit ice nucleation. A hydroxyl distance smaller than ~2.8 Å but greater than ~7.1 Å in modified PVA (MPVA) compounds was associated with the promotion of ice nucleation, while a hydroxyl group separation distance of approximately ~5.0 Å was correlated with a delay in ice nucleation, owing to changes in the energy of the system. Thus, these results may help explain some of the mechanisms of current known anti-freeze compounds and may have implications for designing new anti-freeze compounds in the future.The main objective of this study was to investigate the effect of supplementing beef cattle with incremental levels of Desmanthus leptophyllus cv. JCU1 and Desmanthus bicornutus cv. link2 JCU4 on in vivo methane (CH4) emissions and the role of tannins in rumen fermentation. Fourteen yearling Droughtmaster steers were allocated to each of the two Desmanthus species and offered a basal diet of Rhodes grass (Chloris gayana) hay plus fresh Desmanthus at 0%, 15%, 22%, and 31% of dry matter intake (DMI). The 15% and 31% Desmanthus periods lasted 21 days and the 22 and 0% Desmanthus periods, 14 days. Methane production was measured by open-circuit gas exchange in the last two days of each period. The results showed a linear increase in DMI and reduction in CH4 yield with the increasing level of Desmanthus and subsequently condensed tannins in the diet. The added tannin binder polyethylene glycol-4000 did not affect CH4 yield but increased rumen NH3-N and iso-acid concentrations. Therefore, on a low-quality diet, Desmanthus has the potential to increase intake and reduce CH4 emissions. Even though its tannins can bind rumen proteins, the beef cattle anti-methanogenic response to supplementation with Desmanthus may be a combination of rumen fermentation and tannin effects.The effect of different regimes of combustion of glycine-nitrate precursors on the formation of perovskite phases (LaMnO3 and LaCrO3) without additional heat treatment was studied. The following three combustion regimes were compared the traditional solution combustion synthesis (SCS), volume combustion synthesis (VCS) using a powdered precursor, and self-propagating high-temperature synthesis (SHS) using a precursor pellet. The products of combustion were studied using a series of physicochemical methods (attenuated total reflection infrared spectroscopy (ATR FTIR), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), and thermal analysis). SHS was found to be the most productive regime for the formation of perovskite because of its ability to develop high temperatures in the reaction zone, which led to a reduced content of the thermally stable lanthanum carbonate impurities and to an increased yield and crystallite size of the perovskite phase. The reasons for the better crystallinity and purity of LaCrO3 as compared with LaMnO3 is also discussed, namely the low temperatures of the onset of the thermolysis, the fast rate of combustion, and the favorable thermodynamics for the achievement of high temperatures in the reaction zone.Microencapsulation is a widely studied cell therapy and tissue bioengineering technique, since it is capable of creating an immune-privileged site, protecting encapsulated cells from the host immune system. Several polymers have been tested, but sodium alginate is in widespread use for cell encapsulation applications, due to its low toxicity and easy manipulation. Different cell encapsulation methods have been described in the literature using pressure differences or electrostatic changes with high cost commercial devices (about 30,000 US dollars). Herein, a low-cost device (about 100 US dollars) that can be created by commercial syringes or 3D printer devices has been developed. The capsules, whose diameter is around 500 µm and can decrease or increase according to the pressure applied to the system, is able to maintain cells viable and functional. The hydrogel porosity of the capsule indicates that the immune system is not capable of destroying host cells, demonstrating that new studies can be developed for cell therapy at low cost with microencapsulation production. This device may aid pre-clinical and clinical projects in low- and middle-income countries and is lined up with open source equipment devices.Maternal uniparental disomy of chromosome 7 is present in 5-10% of patients with Silver-Russell syndrome (SRS), and duplication of 7p including GRB10 (Growth Factor Receptor-Bound Protein 10), an imprinted gene that affects pre-and postnatal growth retardation, has been associated with the SRS phenotype. Here, we report on a 17 year old girl referred to array-CGH analysis for short stature, psychomotor delay, and relative macrocephaly. Array-CGH analysis showed two copy number variants (CNVs) a ~12.7 Mb gain in 7p13-p11.2, involving GRB10 and an ~9 Mb loss in 7q11.21-q11.23. FISH experiments performed on the proband's mother showed a chromosome 7 pericentric inversion that might have mediated the complex rearrangement harbored by the daughter. Indeed, we found that segmental duplications, of which chromosome 7 is highly enriched, mapped at the breakpoints of both the mother's inversion and the daughter's CNVs. We postulate that pairing of highly homologous sequences might have perturbed the correct meiotic chromosome segregation, leading to unbalanced outcomes and acting as the putative meiotic mechanism that was causative of the proband's rearrangement. link3 Comparison of the girl's phenotype to those of patients with similar CNVs supports the presence of 7p in a locus associated with features of SRS syndrome.The environmental challenges the world faces nowadays have never been greater or more complex. Global areas covered by forests and urban woodlands are threatened by natural disasters that have increased dramatically during the last decades, in terms of both frequency and magnitude. Large-scale forest fires are one of the most harmful natural hazards affecting climate change and life around the world. Thus, to minimize their impacts on people and nature, the adoption of well-planned and closely coordinated effective prevention, early warning, and response approaches are necessary. This paper presents an overview of the optical remote sensing technologies used in early fire warning systems and provides an extensive survey on both flame and smoke detection algorithms employed by each technology. Three types of systems are identified, namely terrestrial, airborne, and spaceborne-based systems, while various models aiming to detect fire occurrences with high accuracy in challenging environments are studied. Finally, the strengths and weaknesses of fire detection systems based on optical remote sensing are discussed aiming to contribute to future research projects for the development of early warning fire systems.The foodborne disease burden (FBDB) related to 26 major biological hazards in France was attributed to foods and poor food-handling practices at the final food preparation step, in order to develop effective intervention strategies, especially food safety campaigns. Campylobacter spp. and non-typhoidal Salmonella accounted for more than 60% of the FBDB. Approximately 30% of the FBDB were attributed to 11 other hazards including bacteria, viruses and parasites. Meats were estimated as the main contributing food category causing (50-69%) (CI90) of the FBDB with (33-44%), (9-21%), (4-20%) (CI90) of the FBDB for poultry, pork and beef, respectively. Dairy products, eggs, raw produce and complex foods caused each approximately (5-20%) (CI90) of the FBDB. When foods are contaminated before the final preparation step, we estimated that inadequate cooking, cross-contamination and inadequate storage contribute for (19-49%), (7-34%) and (9-23%) (CI90) of the FBDB, respectively; (15-33%) (CI90) of the FBDB were attributed to the initial contamination of ready-to-eat foods-without any contribution from final food handlers.