Carltonmoos1366

Overall, our data suggest that DOX-induced chromosome damage can be a causal factor for insertions of mtDNA in the nuclear genome of human lymphocytes. It can be assumed that the impact of a large number of external and internal mutagenic factors contributes significantly to the origin and amount of mtDNA in nuclear genomes.In times of spreading multidrug-resistant bacteria, species identification and decontamination of cell cultures can be challenging. Here, we describe a mobile cell culture contaminant with "black dot"-like microscopic appearance in newly established irreplaceable hybridoma cell lines and its identification. Using 16S rRNA gene sequencing, species-specific PCRs, whole genome sequencing (WGS), and MALDI-TOF mass spectrometry, the contaminant was identified as the ubiquitous environmental and clinically relevant Gram-negative bacterium Ralstonia insidiosa (R. insidiosa), a strong biofilm producer. Further characterizations by transmission electron microscopy (TEM) and biochemical API test were not conclusive. Whole genome sequencing of our R. insidiosa isolate revealed numerous drug-resistance determinants. Genome-wide comparison to other Ralstonia species could not unambiguously designate our isolate to R. insidiosa ( less then 95% average nucleotide identity) suggesting a potential novel species or subspecies, closely related to R. insidiosa and R. pickettii. After determining the antibiotic susceptibility profile, the hybridoma cell culture was successfully decontaminated with ciprofloxacin without affecting antibody production.In this paper, hot gas pressure forming (HGPF) of Ti-55 high temperature titanium alloy was studied. The hot deformation behavior was studied by uniaxial tensile tests at temperatures ranging from 750 to 900 °C with strain rates ranging from 0.001 to 0.05 s-1, and the microstructure evolution during tensile tests was characterized by electron backscatter diffraction. Finite element (FE) simulation of HGPF was carried out to study the effect of axial feeding on thickness distribution. Forming tests were performed to validate this process for Ti-55 alloy. Results show that when the temperature was higher than 750 °C, the elongation was large enough for HGPF of Ti-55 alloy. Dynamic recrystallization (DRX) occurred during the tensile deformation, which could refine the microstructure. The thickness uniformity of the formed part could be improved by increasing feeding length. The maximum thinning ratio decreased from 27.7% to 11.5% with the feeding length increasing from 0 to 20 mm. A qualified Ti-55 alloy component was successfully formed at 850 °C, the microstructure was slightly refined after forming, and the average post-form yield strength and peak strength were increased by 8.7% and 6.9%, respectively. Pre-heat treatment at 950 °C before HGPF could obtain Ti-55 alloy tubular component with bimodal microstructure and further improve the post-form strength.

Autism spectrum disorder (ASD) is a neurodevelopmental disorder with deficits in social communication ability and repetitive behavior. The pathophysiological events involved in the brain of this complex disease are still unclear.

In this study, we aimed to profile the gene expression signatures of brain cortex of ASD patients, by using two publicly available RNA-seq studies, in order to discover new ASD-related genes.

We detected 1567 differentially expressed genes (DEGs) by meta-analysis, where 1194 were upregulated and 373 were downregulated genes. Several ASD-related genes previously reported were also identified. Our meta-analysis identified 235 new DEGs that were not detected using the individual RNA-seq studies used. Some of those genes, including seven DEGs (

and

), have been confirmed in previous reports to be associated with ASD. Gene Ontology (GO) and pathways analysis showed several molecular pathways enriched by the DEGs, namely, osteoclast differentiation, TNF signaling pathway, complement and coagulation cascade. Topological analysis of protein-protein interaction of the ASD brain cortex revealed proteomics hub gene signatures

and

. We also identified the transcriptional factors (TFs) regulating DEGs, namely,

.

Novel core genes and molecular signatures involved with ASD were identified by our meta-analysis.

Novel core genes and molecular signatures involved with ASD were identified by our meta-analysis.Environmental heat stress poses significant physiological challenge and impairs exercise performance. We investigated the impact of wrist percooling on running performance and physiological and perceptual responses in the heat. In a counterbalanced design, 13 trained males (33 ± 9 years, 15 ± 7% body fat, and maximal oxygen consumption, VO2max 59 ± 5 mL/kg/min) completed three 10 km running time trials (27 °C, 60% relative humidity) while wearing two cooling bands (1) both bands were off (off/off), (2) one band on (off/on), (3) both bands on (on/on). Heart rate (HR), HR variability (HRV), mean arterial pressure (MAP), core temperature (TCO), thermal sensation (TS), and fatigue (VAS) were recorded at baseline and recovery, while running speed (RS) and rating of perceived exertion (RPE) were collected during the 10 km. Wrist cooling had no effect (p > 0.05) at rest, except modestly increased HR (3-5 ∆beats/min, p 0.05). We demonstrate that wrist percooling elicited a faster running speed, though this coincides with increased HR; although, interestingly, sensations of effort and thermal comfort were unaffected, despite the faster speed and higher HR.A well-defined block copolymer brush poly(glycidyl methacrylate)-graft-(poly(methyl methacrylate)-block- poly(oligo(ethylene glycol) methyl ether methacrylate)) (PGMA-g-(PMMA-b-POEGMA)) is synthesized via grafting from an approach based on a combination of click chemistry and reversible addition-fragmentation chain transfer (RAFT) polymerization. The resulting block copolymer brushes were characterized by 1H-NMR and size exclusion chromatography (SEC). The self-assembly of the block copolymer brush was then investigated under selective solvent conditions in three systems THF/water, THF/CH3OH, and DMSO/CHCl3. PGMA-g-(PMMA-b-POEGMA) was found to self-assemble into spherical micelle structures as analyzed by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The average size of the particles was much smaller in THF/CH3OH and DMSO/CHCl3 as compared with the THF/water system. Thin film of block copolymer brushes with tunable surface properties was then prepared by the spin-coating technique. The thickness of the thin film was confirmed by scanning electron microscopy (SEM). Atom force microscopy (AFM) analysis revealed a spherical morphology when the block copolymer brush was treated with poor solvents for the backbone and hydrophobic side chains. The contact angle measurements were used to confirm the surface rearrangements of the block copolymer brushes.In the last decades, a lot of effort has been made in order to improve the use of environmentally friendly and renewable energy sources. In a context of small energy usage, energy harvesting takes place and thermal energy sources are one of its main energy sources because there are several unused heat sources available in the environment that may be used as renewable energy sources. To rapidly evaluate the energy potential of such thermal sources is a hard task, therefore, a way to perform this is welcome. In this work, a thermal pattern emulation system to evaluate potential thermal source in a easy way is proposed. The main characteristics of the proposed system is that it is online and remote, that is, while the thermal-source-under-test is being measured, the system is emulating it and evaluating the generated energy remotely. The main contribution of this work was to replace the conventional Proportional Integral Derivative (PID) controller to a Fuzzy-Proportional Integral (PI) controller. In order to compare both controllers, three tests were carried out, namely (a) step response, (b) perturbation test, (c) thermal emulation of the thermal pattern obtained from a potential thermal source tree trucks. Experimental results show that the Fuzzy-PI controller was faster than the PID, achieving a setting time 41.26% faster, and also was more efficient with a maximum error 53% smaller than the PID.A new strain of Cordyceps javanica (wf GA17) was observed causing widespread epizootics among whiteflies in Southern Georgia in 2017. The tolerance of conidia to environmental factors including variable temperature and ultraviolet (UV) light was compared between this strain and three commercial strains of entomopathogenic fungi (Metarhizium brunneum F52, Cordyceps fumosorosea Apopka97, and Beauveria bassiana GHA). Under 10-30 °C, C. javanica wf GA17 responded similarly to other fungi, with the highest virulence against Galleria mellonella at 25 °C, followed by 20, 30, and 15 °C; lowest virulence was observed at 10 °C. At 35 °C and 40 °C, C. javanica wf GA17 had lower tolerance than M. brunneum F52 and B. bassiana GHA, but was superior to C. Daidzein mw fumosorosea Apopka97 in conidia viability and post-treatment virulence. After exposure to -20 °C for 56 d, C. javanica wf GA17 exhibited lower germination than M. brunneum F52 and lower virulence than M. brunneum F52 and B. bassiana GHA, but higher germination and virulence than C. fumosorosea Apopka97. Following exposure to strong UV light, viability and virulence of all fungi were reduced with increasing exposure periods. Increased environmental tolerance of C. javanica wf GA17 over C. fumosorosea Apopka97 suggests that the new strain could have applicability for commercial pest management.Many companies have transformed their telephone systems into Voice over IP (VoIP) systems. Although implementation is simple, VoIP is vulnerable to different types of attacks. The Session Initiation Protocol (SIP) is a widely used protocol for handling VoIP signaling functions. SIP is unprotected against attacks because it is a text-based protocol and lacks defense against the growing security threats. The Distributed Denial of Service (DDoS) attack is a harmful attack, because it drains resources, and prevents legitimate users from using the available services. In this paper, we formulate detection of DDoS attacks as a classification problem and propose an approach using token embedding to enhance extracted features from SIP messages. We discuss a deep learning model based on Recurrent Neural Networks (RNNs) developed to detect DDoS attacks with low and high-rate intensity. For validation, a balanced real traffic dataset was built containing three attack scenarios with different attack durations and intensities. Experiments show that the system has a high detection accuracy and low detection time. The detection accuracy was higher for low-rate attacks than that of traditional machine learning.Films exhibiting co-crystalline (CC) phases between a polymer host and low-molecular-mass guest molecules are relevant for many applications. As is usual for semi-crystalline polymers, axially oriented films can give relevant information on the crystalline structure, both by Wide Angle X-ray diffraction fiber patterns and by polarized Fourier-transform infrared spectroscopy. Axially oriented CC phases of poly(2,6-dimethyl-1,4-phenylene)oxide (PPO) with 1,3,5-trimethylbenzene (mesitylene) can be simply obtained by the stretching of CC PPO films. In fact, due to the plasticization effect of this highly boiling guest, PPO orientation can occur in a stretching temperature range (170-175 °C) nearly 50 °C lower than that generally needed for PPO films (220-230 °C). This low stretching temperature range allows avoidance of polymer oxidation, as well as formation of the mesomorphic dense γ PPO phase. Axially oriented CC phases of PPO with toluene, i.e., with a more volatile guest, can be instead obtained by the stretching (in the same low temperature range 170-175 °C) of CC PPO blend films with polystyrene.