Loveladegaard0544

The presence of Eschericia coli O157H7 in the natural environment is a serious threat to human health. The native microbial community in soil plays an important role in resisting E. coli O157H7 invasion. This study examined the responses of soil microbial community to E. coli O157H7 invasion during a 32-day incubation. The E. coli O157H7 persisted longer in γ-irradiated soil than non-irradiated soil while glucose addition decreased its persistence in the irradiated soil which was associated with an increasing recovery of the native community. The invasion of E. coli O157H7 increased soil organic carbon mineralization, an indicator of microbial activity, in both non-irradiated and irradiated soils, while glucose addition significantly promoted the carbon mineralization process. The 16S rRNA sequencing data showed the gradual recovery of the native bacterial population including specific taxa such as proteobacteria and actinobacteria following irradiation. It is concluded that soil microbial function and structure can affect persistence of E. coli O157H7 and that lower biodiversity of the native community favors its persistence. The quantitative fate of polybrominated diphenyl ethers (PBDEs) in soil is unknown. Furthermore, the effects of co-contamination by toxic copper on the behavior of PBDEs have not been investigated. Using a 14C-tracer, we studied mineralization, metabolism, and formation of non-extractable residues (NERs) of one PBDE congener, i.e., the 4-bromodiphenyl ether (BDE3) in oxic soil for 50 days, without and with amendment of Cu (400 mg kg-1 soil dw). BDE3 rapidly dissipated with a half-life of 5.5 days and large amounts of CO2 (38.8 ± 0.3% of initial applied amount at the end of incubation) and NERs (42.5 ± 0.4%) were rapidly produced. One hydroxylated metabolite (4'-HO-BDE3) was formed (8.1 ± 0.6%) at the beginning of the incubation, but then decreased to 2.2 ± 0.4%. Only BDE3 occurred in physico-chemically entrapped NERs, amounting to 9.2 ± 0.7%, while only 4'-HO-BDE3 in ester-linked NERs (10.9 ± 0.7%). The addition of Cu strongly reduced the kinetics constants of the transformations (including dissipation, mineralization, and NER-formation), the predicted maximal amounts of mineralization, as well as covalent binding of 4'-HO-BDE3 to soil. The results provide first quantitative insights into the fate of low-brominated congeners of PBDEs in soil and indicate that co-contamination by Cu may increase the environmental risks of biodegradable PBDEs in soil by increasing their persistence. Little is known about how forests adjust their gas-exchange mode while atmospheric CO2 rises globally and air quality changes regionally. The present study aims at addressing this research gap for boreal spruce trees growing in three different regions of Canada, submitted to distinct levels of atmospheric emissions, by examining the amount of carbon gained per unit of water lost in trees, i.e., the intrinsic water use efficiency (iWUE). Under pristine air quality conditions, middle-to long-term trends passed from no-reaction mode to passive strategies due to atmospheric CO2, and short-term iWUE variations mostly ensue from year-to-year climatic conditions. In contrast, in trees exposed to pollutants from a copper smelter and an oil-sands mining region, air quality deterioration generated swift, long-term iWUE rises immediately at the onset of operations. In this case, the very active foliar strategy sharply reduced the intra-foliar CO2 (Ci) pressure. Statistical modeling allowed identifying emissions as the main trigger for the iWUE swift shifts; subsequent combined effects of emissions and rising CO2 led to passive foliar modes in the recent decades, and short-term variations due to climatic conditions appeared all along the series. Overall, boreal trees under different regional conditions modified their foliar strategies mostly without changing their stem growth. These findings underline the potential of acidifying emissions for prompting major iWUE increases due to lowering the stomatal apertures in leaves, and the combined influence of rising CO2 in modulating other foliar responses. A fallout of this research is that degrading air quality may create true divergences in the relationship between tree-ring isotopes and climatic conditions, an impact to consider prior to using isotopic series for paleo-climatic modeling. Exposure to benzo(a)pyrene (BaP) has been shown to cause mitochondrial dysfunction and injury to neural cells. Resveratrol (RSV) has been studied as an antioxidant, anti-inflammatory, anti-apoptotic, and anticancer agent and can modulate mitochondrial function in vitro and in vivo. However, the molecular mechanisms underlying RSV's protection against mitochondrial dysfunction have not been fully elucidated. To investigate whether RSV can effectively prevent BaP-induced mitochondrial dysfunction, we tested the effects of RSV in primary neuronal models. Our results confirmed that neurons exhibited mitochondrial dysfunction and apoptosis in the mitochondrial pathway after BaP-treatment, and that pretreatment with RSV could reduce that dysfunction. Further, our results indicated that RSV pretreatment enhanced mitochondrial biogenesis via the AMPK/PGC-1α pathway and activated mitophagy via the PINK1-Parkin and AMPK/ULK1 pathways, thereby coordinating mitochondrial homeostasis. We also found that RSV could alleviate mitochondrial network fragmentation caused by BaP. This work provided insights into the role of RSV in preventing BaP-induced primary neuronal apoptosis in the mitochondrial pathway, mainly via regulation of mitochondrial biogenesis and mitophagy through AMPK pathway, thus maintaining the integrity of the mitochondrial network. Antibiotic resistance surveillance data is lacking in many parts of the world, limiting effective therapy and management of resistance development. Analysis of urban wastewater, which contains bacteria from thousands of individuals, opens up possibilities to generate informative surveillance data in a standardized and resource-efficient way. Here, we evaluate the relationship between antibiotic resistance prevalence in E. coli from wastewater and clinical samples by studying countries with different resistance situations as assessed by traditional clinical surveillance. Composite, influent wastewater samples were collected over 24 h from treatment plants serving major cities in ten European countries. Using a broth screening method, resistance to six antibiotic classes was analyzed for 2507 E. coli isolates (n = 247-252 per country). Resistance prevalence in wastewater E. coli was compared to that in clinical E. coli reported by the European Antibiotic Resistance Surveillance Network. Resistance prevalence was lower in wastewater than clinical E. coli but followed similar geographic trends. Significant relationships were found for resistance to aminopenicillins (R2 = 0.72, p = 0.0019) and fluoroquinolones (R2 = 0.62, p = 0.0072), but not for aminoglycosides (R2 = 0.13, p = 0.31) and third-generation cephalosporins (R2 = 0.00, p = 0.99) where regression analyses were based on considerably fewer resistant isolates. When all four antibiotic classes were taken into account, the relationship was strong (R2 = 0.85, p  less then  0.0001). Carbapenem resistance was rare in both wastewater and clinical isolates. Wastewater monitoring shows promise as method for generating surveillance data reflecting the clinical prevalence of antibiotic resistant bacteria. Such data may become especially valuable in regions where clinical surveillance is currently limited. Intertidal bivalves are periodically exposed in air. It is tempting to speculate that the organisms would temporarily escape from contaminants when they are out of water and thus have lower risks. In this study, we tested this speculation by investigating cadmium (Cd) toxicokinetics in an intertidal mussel, Xenostrobus atratus, under the effects of tidal exposure using simulated tidal regimes. The uptake rate constant (ku) of Cd ranged from 0.045 L g-1 d-1 to 0.109 L g-1 d-1, whereas the elimination rate constant (ke) of Cd ranged from 0.029 d-1 to 0.091 d-1. Cd bioaccumulation was slightly higher in the continuously immersed mussels than the alternately immersed mussels, but much lower than what would be expected if assuming bioaccumulation being proportional to immersion duration. Cd uptake was observed even when mussels were exposed in air, due to uptake of Cd dissolved in mantle cavity fluid and internalization of Cd adsorbed on mussel tissues. Overall, tidal height showed limited effects on Cd bioaccumulation, consistent with the trend of Cd concentrations found in X. atratus collected from different tidal heights. The mantle cavity uptake mechanism is expected to be applicable to other contaminants and bivalves, and should have important implications in risk assessments for intertidal environment. SBI-0640756 concentration This work aims to study the pseudo-total content, geochemical fractions, and species of arsenic (As) in the bulk soil and in the coarse and fine particles of top soil and soil profiles collected from active and abandoned gold mine spoils in Ghana. The human health risk for adults (male and female) and children has been assessed. To achieve our aims, we collected 51 samples, characterized them, determined the total As content, and sequentially extracted the geochemical fractions of As including water-soluble and un-specifically bound As (FI); specific-sorbed/exchangeable As (FII); poorly (FIII)- and well-crystalline (IV) Fe oxide; and residual/sulphide fraction (FV). In selected samples, As species were determined using synchrotron-based X-ray absorption near edge structure (XANES). Pseudo-total As contents varied from 1807 to 8400 mg kg-1, with the extremes occurring at the abandoned mine spoil. Arsenic was almost 10-fold higher in the fine particles ( less then 0.63 μm) than in the coarse particles. Arsenic was mainly associated with FIII and FV, indicating that the distribution of As in these spoils is governed by their contents of amorphous Fe oxides, sulphides and As bearing minerals. The XANES results indicated that scorodite (FeAsSO4 = 65-76%) and arsenopyrite (FeAsS = 24-35%) are the two major As-containing minerals in the spoils. The potential mobility (PMF = ∑FI-FIV) of As in the fine particles of the top soil was higher (48-61%) than in the coarse particles (25-44%). The mobile fraction (MF) (FI+FII) and PMF of As in the coarse particles of the profiles increased with depth while it decreased in the fine particles. The median hazard index values indicated an elevated human health risk, especially for children. The high contamination degree and potential mobility of As at the studied mine spoils indicate high potential risk for human and environmental health. Environmentally persistent free radicals (EPFRs) are easily generated in the combustion processes of municipal solid waste (MSW) and can cause adverse effects on human health. This study focuses on understanding the toxicity of EPFR particles (ZnO/MCB containing EPFRs) to human bronchial epithelial cell lines BEAS-2B and 16HBE, murine macrophages Raw264.7, and the lung of BALB/c mice after a short exposure (7 days). Exposure of BEAS-2B, 16HBE, and Raw264.7 cells to ZnO/MCB particles significantly increased the reactive oxygen species (ROS) production and perturbed levels of intracellular redox conditions (decreased the intracellular GSH level and the activity of cytosolic SOD, and stimulated oxidative stress related proteins such as HO-1 and Nrf2). EPFR particles decreased the mitochondrial membrane potential (MMP) and induced cell apoptosis, including the activation of Caspase-3, Bax, and Bcl-2 apoptotic signalling pathways. A signature inflammatory condition was observed in both cell models and the mouse model for lung lesions.