Hooverpalmer7858

05). CsA inhibited the expression of most genes associated with inflammatory response in NHDFs. There were only 19 genes with a fold change (FC) lower than -2.0, among which EGR1, FOS, PBK, CDK1 and TOP2A had the lowest expression, as did CXCL2 which can directly impact inflammation. Furthermore, ZNF451 was strongly induced, and COL1A1, COL3A1, IL33, TNFRSFs were weakly upregulated (FC lower than 2.0). CONCLUSION The CsA in therapeutic concentration influence the genes linked to inflammatory response (in the transcriptional level) in human dermal fibroblasts. The findings suggest that the potential mechanism of CsA action in this concentration and on these genes can be associated with a profibrotic and proapoptotic, and genotoxic effects. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND Semaphorin 3F (SEMA3F) plays a substantial role in carcinogenesis, because of its role in inducing angiogenesis, and also creating a microenvironment for the developing tumor. OBJECTIVE The purpose of this work was to assess the impact of cisplatin depending on the concentration and exposure time on the expression pattern of SEMA3F in an endometrial cancer cell line. MATERIALS AND METHODS Cultures of the Ishikawa endometrial cancer cells were incubated with cisplatin with the following concentrations 2.5µM; 5µM; and 10µM and for the following periods of time 12; 24; and 48 hours. Cells not incubated with the drug constituted the control in the experiment. To determine the effect of cisplatin on the expression of SEMA3F the real-time quantitative reverse transcription reaction (RtqPCR; mRNA) was used, as well as the ELISA assay (protein). The statistical analysis was done with the admission of p less then 0.05. RESULTS The silencing of SEMA3F expression on the transcriptome and proteome levels in a culture unexposed to the effects of cisplatin in comparison to endometrial cancer cells under the influence of cisplatin (p less then 0.05) were noted. Along with an increase in the concentration of the drug used, the number of copies of the gene transcript, during the shortest incubation period had a gradual increase. Only for the highest concentration of the drug, substantial statistical differences in the expression of the SEMA3F protein between 24 and 48 hour incubation periods (p less then 0.05) were determined. CONCLUSIONS Using cisplatin in an endometrial cancer cell culture results in an increased expression of SEMA3F, which advantageously affects the normalization of the neoplastic angiogenic process and lowers the proliferation of the cells making up the mass of the tumor. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.OBJECTIVES To investigate the effect of Danggui-Shaoyao-San (DSS)-containing serum on the renal tubular epithelial-mesenchymal transition (EMT) of diabetic nephropathy (DN) in high glucose- induced HK-2 cells and its mechanism. METHODS 20 rats were randomly divided into four groups blank control group, DSS low dose group (DSS-L), DSS middle dose group (DSS-M), and DSS high dose group (DSS-H). DSS was administrated to the corresponding group (7g/kg/d, 14g/kg/d and 21g/kg/d) for 7 consecutive days, and the same volume of saline was given to the blank control group by gavage. The rat drugcontaining serum was successfully prepared. HK-2 cells were divided into five groups blank control group, model group, DSS-L, DSS-M, DSS-H, according to the corresponding drug and dose of each treatment group. Protein and mRNA levels of Jagged1, Notch1, Hes5, Notch intracellular domain (NICD), E-cadherin, alpha-smooth muscle actin (α-SMA) and vimentin at 24h, 48h and 72h were detected by Western Blot and RT-qPCR. RESULTS The protein and mRNA levels of Jagged1, Notch1, Hes5, NICD, α-SMA and vimentin in treatment groups were remarkably decreased compared with the model group (P less then 0.05), and the protein and mRNA levels of E-cadherin were notably increased (P less then 0.05) by Western Blot and RTqPCR. CONCLUSION Our results demonstrated that DSS could prevent DN by ameliorating renal tubular EMT through inhibition of Notch signaling pathway. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.Osteosarcoma is considered as one of the most common types of bone tumors occurs among adolescents and children. Current therapy strategies still have limited effectiveness; therefore, the development of new therapies is urgent. Morusin is a compound isolated from Morus australis (Moraceae). Many studies have reported its anti-tumor effect on several tumor types. However, its role in osteosarcoma is still unclear. In this study, we determined that morusin significantly suppresses the proliferation of osteosarcoma cells. Morusin also promotes the apoptosis of osteosarcoma cells. selleckchem Furthermore, the migration and invasion of osteosarcoma were reduced after exposure to morusin. The deep mechanism was determined to be the inhibition of the PI3K/AKT signaling pathway. In conclusion, our study indicates morusin as a potential candidate for osteosarcoma therapy. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.AIM AND OBJECTIVE Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation and remission processes in LN have not been clarified, but is of great need in clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone. METHODS Mrl-lps mice were intervened with LPS, dexamethasone and normal saline(NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. kidney tissue were collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt-PCR (real time Polymerase Chain Reaction) were used for verification. RESULTS We identified 136 proteins that marked quantitative information.