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Our work reveals a role for RNF220/ZC4H2 in regulating LC-NA neuron development, and this finding may be helpful for understanding the pathogenesis of ZC4H2 mutation-associated intellectual disability. © 2020. Published by The Company of Biologists Ltd.Vertebrate heart development requires the integration of temporally distinct differentiating progenitors. However, few signals are understood that restrict the size of the later-differentiating outflow tract (OFT). We show that improper specification and proliferation of second heart field (SHF) progenitors in zebrafish lazarus (lzr) mutants, which lack the transcription factor Pbx4, produces enlarged hearts owing to an increase in ventricular and smooth muscle cells. Specifically, Pbx4 initially promotes the partitioning of the SHF into anterior progenitors, which contribute to the OFT, and adjacent endothelial cell progenitors, which contribute to posterior pharyngeal arches. Subsequently, Pbx4 limits SHF progenitor (SHFP) proliferation. Single cell RNA sequencing of nkx2.5+ cells revealed previously unappreciated distinct differentiation states and progenitor subpopulations that normally reside within the SHF and arterial pole of the heart. Specifically, the transcriptional profiles of Pbx4-deficient nkx2.5+ SHFPs are less distinct and display characteristics of normally discrete proliferative progenitor and anterior, differentiated cardiomyocyte populations. Therefore, our data indicate that the generation of proper OFT size and arch arteries requires Pbx-dependent stratification of unique differentiation states to facilitate both homeotic-like transformations and limit progenitor production within the SHF. © 2020. Published by The Company of Biologists Ltd.The expression of the transcriptional repressor Hes1 oscillates in many cell types, including neural progenitor cells (NPCs), but the significance of Hes1 oscillations in development is not fully understood. To examine the effect of altered oscillatory dynamics of Hes1, we generated two types of Hes1 knock-in mice, a shortened (type-1) and an elongated (type-2) Hes1 gene, and examined their phenotypes focusing on neural development. Although both mutations affected Hes1 oscillations, the type-1 mutation dampened Hes1 oscillations more severely, resulting in much lower amplitudes. The average levels of Hes1 expression in type-1 mutant NPCs were also lower than in wild-type NPCs but similar to or slightly higher than those in Hes1 heterozygous mutant mice, which exhibit no apparent defects. Whereas type-2 mutant mice were apparently normal, type-1 mutant mice displayed smaller brains than wild-type mice and upregulated proneural gene expression. Furthermore, proliferation of NPCs decreased and cell death increased in type-1 mutant embryos. When Hes3 and Hes5 were additionally deleted, neuronal differentiation was also accelerated, leading to microcephaly. Thus, robust Hes1 oscillations are required for maintenance and proliferation of NPCs and the normal timing of neurogenesis, thereby regulating brain morphogenesis. © 2020. Published by The Company of Biologists Ltd.INTRODUCTION The Istanbul Protocol is a document prepared by many scientists from different countries and accepted by the United Nations thematising the medical reporting of torture. Normally, forensic specialists are responsible for medical examinations of torture victims, but this study looks at forensic specialists who were themselves subject to torture and mistreatment. It aims to analyze and evaluate, especially with regard to the Istanbul Protocol, the medical examinations performed on them while they were in detention. MATERIAL/METHOD This study includes experts in forensic science who were detained during and after the state of emergency that was declared in 2016 in Turkey. Participants were asked questions regarding the examination steps specified in the Istanbul protocol. The Google Forms system (Google Inc., CA/USA) was used for the surveys, sent to the participants via text message. RESULTS Twenty-two forensic scientists who were detained during and after the state of emergency in Turkey participan law, it has not completely disappeared. Medical personnel play an important role in torture assesment and prevention. The testimony of the forensic experts, who were fired and detained, revealed that the medical examinations during and after the State of Emergency in Turkey did not comply with the Istanbul Protocol and ethical rules. Medical examinations not carried out in accordance with the Istanbul Protocol lead to the denial of many rights and health problems. A worldwide awareness and sensitivity is needed to solve this problem. In this study, the occurrences of 110 micropollutants in the surface waters and sediments collected at eight sampling sites along the middle reaches of the Yarlung Tsangpo River were investigated in winter. A total of 47 and 45 micropollutants were detected in at least one water or sediment sample, respectively, and their total concentrations reached 790.2 ng/L and 186.5 ng/g on a dry weight basis, respectively. RG-7112 inhibitor Their composition profiles demonstrated that the majority of micropollutants were polycyclic aromatic hydrocarbons (PAHs) and UV filters. The mixture risk quotient (MRQ) values of the detected micropollutants regularly exceeded 1 for aquatic organisms at all sampling sites, and fish and invertebrates are the more sensitive organisms. The diversity and evenness of the zooplankton levels had a clear negative correlation with the micropollutant occurrences in water. The top 10 mixture components belonging to the UV filter and PAH categories explained more than 80% and 95% of the mixture risk for chronic and acute toxicology, respectively. This study is the first investigation of the presence and risk assessment of 110 micropollutants in the Yarlung Tsangpo River Basin and offers new insights into the ecological security of the water resources of the Tibetan Plateau. The traditional disposal methods of paper/wood industry raise serious environmental concerns, thus, requires innovative and productive ideas to manage such waste. This article deals with the appraisal and modification of lignosulphonate, a waste by-product of paper/wood industry, as a soil stabilizer to mitigate the disastrous environmental vulnerabilities of expansive soil related to the wetting-drying cycles. In this context, a novel approach of integrating lignosulphonate with hydrated lime was proposed, based on the short comings of lignosulphonate as a lone soil stabilizer. Periodic variations of wetting-drying cycles were assessed on various engineering properties of untreated and treated expansive soils with the optimum percentage of lignosulphonate, hydrated lime, and proposed binary admixture. Micro-fabric changes were also analyzed to evaluate the stabilization mechanism in mitigating the disastrous environmental aspects of expansive soil. The results showed that both untreated and lignosulphonate treated samples underwent suppression in swelling behavior and gain equilibrium at the third wetting-drying cycle.