Viborgyates8101

Transferring desired genes from wild species to cultivars through alien addition lines (AALs) has been shown to be an effective method for genetic improvement. Cucumis hystrix Chakr. (HH, 2n = 24) is a wild species of Cucumis that possesses many resistant genes. A synthetic allotetraploid species, C. hytivus (HHCC, 2n = 38), was obtained from the cross between cultivated cucumber, C. sativus (CC, 2n = 14), and C. hystrix followed by chromosome doubling. this website Cucumis sativus - C. hystrix AALs were developed by continuous backcrossing to the cultivated cucumbers. In this study, 10 different types of AALs (CC-H01, CC-H06, CC-H08, CC-H10, CC-H12, CC-H06+H09, CC-H06+H10, CC-H06+H12, CC-H08+H10, CC-H01+H06+H10) were identified based on the analysis of fluorescence in situ hybridization (FISH) and molecular markers specific to C. hystrix chromosomes. And the behavior of the alien chromosomes in three AALs (CC-H01, CC-H06+H10, CC-H01+H06+H10) at meiosis was investigated. The results showed that alien chromosomes paired with C. sativus chromosome in few pollen mother cells (PMCs). Further, disomic alien addition lines (DAALs) carrying a pair of C. hystrix chromosome H10 were screened from the selfed progenies of CC-H10. Chromosome pairing between genomes provides cytological evidence for the possible introgression of alien chromosome segments. The development of AALs could serve as a key step for exploiting and utilizing valuable genes from C. hystrix.DIUTHAME (desorption ionization using through hole alumina membrane) is a novel matrix-free laser desorption/ionization method that enables highly reproducible acquisition of mass spectra. This study aims to evaluate the applicability of DIUTHAME to the acetylcholinesterase reaction assay (AChE assay) commonly used in high-throughput screening (HTS) in the drug discovery process. The commercially available 9-ch DIUTHAME chip was applied to a series of AChE assays prepared with different reaction times. Numerous positive-mode TOF mass spectra were acquired from the raster-scanned sample spots of the AChE assays to analyze the progress of the enzyme reaction and to perform mass spectrometry imaging of the sample spots. The reaction kinetics plots obtained by DIUTHAME were found to reflect the time course of reaction progression as much as those obtained by MALDI and were found to have less error variation than MALDI. DIUTHAME allowed the already irradiated sample spots to be reused to reproduce the reaction kinetics plots by the second measurement conducted a week after, whereas MALDI was not able to properly reproduce the kinetics plots by remeasuring the irradiated sample spots. In DIUTHAME, which does not require a matrix, the experimental procedure for measuring AChE assay becomes extremely simple. DIUTHAME is potentially able to provide more precise AChE reaction kinetics plots than what have been accomplished by MALDI.To enable rapid development of flexible microelectronic systems, effective thermal management is needed. Flexible polydimethylsiloxane (PDMS)-based microchannel flow boiling may provide a desirable solution. However, the heat transfer performance of PDMS-based microchannels is diminished by its poor thermophysical properties. The development of PDMS wick is proposed to address this dilemma. Herein, a new PDMS wick structure is designed and integrated in the microfluidic device to significantly enhance its thermal performance by promoting capillary-driven flow. Furthermore, to achieve highly efficient vapor removal, a dedicated vapor pathway is designed in the microfluidic device. Experiments have been conducted to investigate the capillary-assisted evaporation/boiling for mass flux ranging from 70 to 245 kg/m2 s on dielectric fluid HFE-7100. The capillary-assisted sustainable and stable thin film evaporation and efficient vapor removal have been demonstrated through the visualization studies. With this new wick design, the liquid rewetting at global and local levels is facilitated through the capillary-driven flow and the efficient vapor removal. This hybrid liquid rewetting mechanism is experimentally demonstrated to significantly enhance the capillary-assisted evaporation/boiling in PDMS-based microchannel. A critical heat flux (CHF) of 14.7 W/cm2 is achieved at a mass flux of 245 kg/m2 s. The heat transfer coefficients (HTC) range from 2000 to 9800 W/m2 K. These values are comparable to that in copper/silicon microchannels, with the aforementioned benefits of flexibility. Equally importantly, stable two-phase transport is achieved as well.Topochemical reduction of the n = 1 Ruddlesden-Popper phases LaSrCo0.5Rh0.5O4 and LaSrNi0.5Rh0.5O4 with Zr yields LaSrCo0.5Rh0.5O3.25 and LaSrNi0.5Rh0.5O3.25, respectively. Magnetization and XPS data reveal that while the rhodium centers in LaSrCo0.5Rh0.5O3.25 and LaSrNi0.5Rh0.5O3.25 have an average oxidation state of Rh2+, these are actually mixed valence Rh(I,III) compounds, with the disproportionation of Rh2+ driven by the favorability of locating d8 Rh1+ and d6 Rh3+ cations within square-planar and square-based pyramidal coordination sites, respectively.The absorption behavior between plasmonic nanostructures and a target molecule plays key roles in effective surface-enhanced Raman scattering (SERS) detection. However, for analytes with low surface affinity to the metallic surface, e.g., polycyclic aromatic hydrocarbons (PAHs), it remains challenging to observe the enhanced Raman signal. In this work, we reported a ratiometric SERS strategy for sensitive PAH detection through the surface functionalization of 3D ordered mesoporous Au nanoparticles (meso-Au NPs). By employing mono-6-thio-β-cyclodextrin (HS-β-CD) as capture ligands, the hydrophobic molecules, e.g., anthracene, could be effectively absorbed on the meso-Au NP surface via a host-guest interaction. Besides, a hydrophobic slippery surface is used as a concentrator to deliver and enrich the Au/analyte droplets into a small area. Consequently, the detection limits of anthracene and naphthalene are down to 1 and 10 ppb. The improved SERS enhancement is mainly ascribed to the host-guest effect of HS-β-CD ligands, large surface area and high-density of sub-10 nm mesopores of Au networks, as well as the enrichment effect of hydrophobic slippery surface. Moreover, the HS-β-CD (480 cm-1 band) could serve as an internal standard, leading to the ratiometric determination of anthracene ranging from 1 ppm to 1 ppb. The proposed surface modification strategy in combination with the hydrophobic slippery surface shows great potential for active capture and trace detection of persistent organic pollutants in real-world SERS applications.We demonstrate that two-photon excitations to bipolariton states created by placing several molecules in an optical cavity can be manipulated by quantum light. Entangled photons can access classically dark bipolariton states by modifying the quantum interferences of two-photon transition pathways involving different single-polariton intermediate states and time-ordering of the two photon beams.CN is known for its fast reactions with hydrocarbons at low temperatures, but relatively few studies have focused on the reactions between CN and aromatic molecules. The recent detection of benzonitrile in the interstellar medium, believed to be produced by the reaction of CN and benzene, has ignited interest in studying these reactions. Here, we report rate constants of the CN + toluene (C7H8) reaction between 15 and 294 K using a CRESU (Cinétique de Réaction en Ecoulement Supersonique Uniforme; reaction kinetics in uniform supersonic flow) apparatus coupled with the pulsed laser photolysis-laser-induced fluorescence (PLP-LIF) technique. We also present the stationary points on the potential energy surface of this reaction to study the available reaction pathways. We find the rate constant does not change over this temperature range, with an average value of (4.1 ± 0.2) × 10-10 cm3 s-1, which is notably faster than the only previous measurement at 105 K. While the reason for this disagreement is unknown, we discuss the possibility that it is related to enhanced multiphoton effects in the previous work.The kinetics of the reactions of PO with O2 and PO2 with O3 were studied at temperatures ranging from ∼190 to 340 K, using a pulsed laser photolysis-laser induced fluorescence technique. For the reaction of PO + O2, there is evidence of both a two- and three-body exit channel, producing PO2 + O and PO3, respectively. Potential energy surfaces of both the PO + O2 and PO2 + O3 systems were calculated using electronic structure theory and combined with RRKM calculations to explain the observed pressure and temperature dependences. For PO + O2, at pressures typical of a planetary upper atmosphere where meteoric ablation of P will occur, the reaction is effectively pressure independent with a yield of PO2 + O of >99%; the rate coefficient can be expressed by log10(k, 120-500 K, cm3 molecule-1 s-1) = -13.915 + 2.470 log10(T) - 0.5020(log10(T))2, with an uncertainty of ±10% over the experimental temperature range (191-339 K). With increasing pressure, the yield of PO3 increases, reaching ∼90% at a pressure of 1 atm and T = 300 K. For PO2 + O3, k(188-339 K) = 3.7 × 10-11 exp(-1131/T) cm3 molecule-1 s-1, with an uncertainty of ±26% over the stated temperature range. Laser-induced fluorescence spectra of PO over the wavelength range 245-248 nm were collected and simulated using pgopher to obtain new spectroscopic constants for the ground and v = 1 vibrational levels of the X2Π and A2Σ+ states of PO.The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics. However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus. We seek to address this limitation by adopting a recombinant cellulosome strategy for this host. In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface. Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study. Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins. Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.Pheromone-binding proteins (PBPs) are small, water-soluble proteins found in the lymph of pheromone-sensing hairs. PBPs are essential in modulating pheromone partitioning in the lymph and at pheromone receptors of olfactory sensory neurons. The function of a PBP is associated with its ability to structurally convert between two conformations. Although mechanistic details remain unclear, it has been proposed that the structural transition between these forms is affected by two factors pH and the presence or absence of ligand. To better understand the PBP conformational transition, the structure of the gypsy moth (Lymantria dispar) LdisPBP1 was elucidated at pH 4.5 and 35 °C using nuclear magnetic resonance spectroscopy. In addition, the effects of sample pH and binding of the species' pheromone, (+)-disparlure, (7R,8S)-epoxy-2-methyloctadecane, and its enantiomer were monitored via 15N HSQC spectroscopy. LdisPBP1 in acidic conditions has seven helices, with its C-terminal residues forming the seventh helix within the pheromone-binding pocket and its N-terminal residues disordered.