Abdibuhl8428

Laccase-based biosensors were successfully prepared using innovative, cheap, one-step Soft Plasma Polymerization technique by deposition of a bio-recognition layer on glassy carbon electrode and MWCNT (Multi-walled Carbon Nanotubes)-modified glassy carbon electrode. The Soft Plasma Polymerization technique is based on corona discharge of cold atmospheric plasma with close to room temperature. The presented work includes study of biosensor working conditions, optimization of the voltage value applied for corona discharge generation as well as applicability and interference studies for dopamine determination. The biosensor constructed under optimal conditions (corona discharge generated at a voltage of 3 kV and in 30 s time deposition, helium flow rate 10 L/min, laccase solution flow rate 200 μL/min) has two linear ranges from 0.1 μmol/dm3 to 10 μmol/dm3 and from 10 μmol/dm3 to 50 μmol/dm3 with dopamine detection sensitivities of 3.63 μA*dm3/μmol and 1.33 μA*dm3/μmol. Application of the MWCNT interlayer allows the dopamine detection sensitivity to be significantly increased to 22.35 μA* dm3/μmol for a linear range from 0.1 μmol/dm3 to 6 μmol/dm3. Additionally, the studied biosensors have stable and anti-interference ability. Both biosensors were successfully applied for dopamine determination in pharmaceutical preparation.A technique for implementing biocompatible and antibacterial functions to a targeted region on tooth surfaces has potential in dental treatments. We have recently demonstrated pseudo-biomineralization, i.e., the growth of an apatite layer on a human dentin substrate by a laser-assisted biomimetic (LAB) process, based on pulsed laser irradiation in a supersaturated CaP solution. In this study, pseudo-biomineralization was induced in the presence of fluoride ions using the LAB process in order to fabricate an antibacterial fluoride-incorporated apatite (FAp) layer on the dentin surface. Tofacitinib After processing for 30 min, a micron-thick FAp layer was formed heterogeneously at the laser-irradiated solid-liquid interface via pseudo-biomineralization. A time-course study revealed that the LAB process first eliminated the pre-existing organic layer, while allowing fluoride incorporation into the dentin surface within 1 min. Within 5 min, FAp nanocrystals precipitated on the dentin surface. Within 30 min, these nanocrystals acquired a pillar-like structure that was weakly oriented in the direction normal to the substrate surface to form a dense micron-thick layer. This layer was integrated seamlessly with the underlying dentin without any apparent gaps. The FAp layer exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. The proposed LAB process is expected to be a useful new tool for tooth surface functionalization via facile and area-specific pseudo-biomineralization.The endothelialization on biomaterial surface has been seen as an important strategy to solve the clinic problems with the cardiovascular implant device. However, the continuous and large surfaces such as artificial heart or artificial cardiac valve cannot maintain the structural and functional stability of the endothelium without the supply of substratum structures. Herein, we combined the micro/nano technology of material surface engineering and the tissue engineering technology to construct the biomimetic vascular endothelial substratum for high quality and complete endothelialization through inducing self-organized differentiation from MSCs to SMCs, controlling their self-aggregation structure and further manipulating micro-tissue on the surface. In the present work, the micro/nano two-scale features of surface were manipulated by preparing the micro arrays of TiO2 nanotubes on titanium surface. The responses of MSCs to these surfaces revealed that the MSCs could be highly regulated and then their self-organized differentiation to SMCs could be induced and improved based on anchoring of the adhesion complex protein and traction of F-actin adjusted by the micro/nano features of the surfaces. Besides, SMCs' self-aggregation structure could also be adjusted effectively by manipulating micro/nano features on two-scale surfaces, and three types of tissue-like structures could be achieved for the further use in formation and surface manipulation of micro-tissue and biomimetic construction of vascular endothelial substratum.Host immune response to tissue engineering tissues or organs directly determines the graft survival and the integration with host. Our and other previous studies have successfully regenerated the organs/tissues based on allogeneic native decellularized matrix (aNDM). But the very limited aNDM clinically hinders the artificial organs/tissues application to resolve the native organs/tissues loss with high incidence. However, the xenogeneic NDM will induce host immune rejection leading to the transplantation failure. This study constructed the xenogeneic (porcine) NDM (xNDM) which carried the immunoregulator Rosiglitazone (xNDM-RSG), a synthetic highly selective agonist of peroxisome proliferator-activated receptor-γ (PPARγ), evaluated xNDM's physical and chemical characterization, immunomodulatory properties, and its effect on the tissue regeneration. Results showed that the xNDM-RSG did not affect the proliferation and differentiation of odontogenic stem cells. In addition, the xNDM-RSG could also effectively decrease the expression of IL-1 and TNFα, and increase the expression of IL-10 and TGFβ to enable a favorable immunomodulation and promote the ligament-to-bone regeneration by PPARγ to induce the alternative activated macrophages (M2 macrophages) antagonizing classically activated macrophages (M1 macrophages). Meanwhile the xNDM-RSG obviously reduces the implants absorption and promotes the regenerated ligament-to-bone expressing the key proteins (ALP, OPN, DSP) which are relative to the native dental and bone. This study demonstrated that protein adsorption could aggravate the immune inflammatory reaction, whereas, xNDM-RSG could effectively control the host immune response to accelerate tissue reparation and regeneration by facilitating the macrophage polarization, which highlighted a new strategy for improving the transplantation survival of the artificial organ or tissue based on the xenogeneic decellularized biomaterials.