Colonbraun3754

Typically, it exhibits a potential gap of 0.662 V for OER/ORR and enables an alkaline water electrolyzer with a cell voltage of 1.558 V at 10 mA cm-2. This work would serve as guidance for well construction of transition-metal-based trifunctional electrocatalysts by the MOF-assisted strategy or the modulation effects of low-content Ru.Thin-film continuous composition spreads of Fe-Co-O were fabricated by reactive cosputtering from elemental Fe and Co targets in reactive Ar/O2 atmosphere using deposition temperatures ranging from 300 to 700 °C. Fused silica and platinized Si/SiO2 strips were used as substrates. Ti and Ta were investigated as adhesion layer for Pt and the fabrication of the Fe-Co-O films. The thin-film composition spreads were characterized by high-throughput electron-dispersive X-ray spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, atomic force microscopy, scanning electron microscopy, and optical transmission spectroscopy. The Fe-content ranged from 28 to 72 at. %. The spinel phases Fe2CoO4 and FeCo2O4 could be synthesized and stabilized at all deposition temperatures with a continuous variation in spinel composition in between. The dependence of the film surface microstructure on the deposition temperature and the composition was mapped. Moreover, the band gap values, ranging from 2.41 eV for FeCo2O4 to 2.74 eV for Fe2CoO4, show a continuous variation with the composition.Two-dimensional (2D) materials exhibit exceptional physical and chemical properties owing to their atomically thin structures. However, it remains challenging to produce 2D materials consisting of pure monoelemental metallic atoms. Here free-standing 2D gold (Au) membranes were prepared via in situ transmission electron microscopy straining of Au films. The applied in-plane tensile strain induces an extensive amount of out-of-plane thinning deformation in a local region of an Au thin film, resulting in the nucleation and growth of a free-standing 2D Au membrane surrounded by its film matrix. This 2D membrane is shown to be one atom thick with a simple-hexagonal lattice, which forms an atomically sharp interface with the face-centered cubic lattice of the film matrix. Diffusive transport of surface atoms, in conjunction with the dynamic evolution of interface dislocations, plays important roles in the formation of 2D Au membranes during the mechanical thinning process. These results demonstrate a top-down approach to produce free-standing 2D membranes and provide a general understanding on extreme mechanical thinning of metallic films down to the single-atom-thick limit.Noncovalent interactions of aromatic surfaces play a key role in many biological processes and in determining the properties and utility of synthetic materials, sensors, and catalysts. However, the study of aromatic interactions has been challenging because these interactions are usually very weak and their trends are modulated by many factors such as structural, electronic, steric, and solvent effects. Recently, N-arylimide molecular balances have emerged as highly versatile and effective platforms for studying aromatic interactions in solution. These molecular balances can accurately measure weak noncovalent interactions in solution via their influence on the folded-unfolded conformational equilibrium. The structure (i.e., size, shape, π-conjugation, and substitution) and nature (i.e., element, charge, and polarity) of the π-surfaces and interacting groups can be readily varied, enabling the study of a wide range of aromatic interactions. These include aromatic stacking, heterocyclic aromatic stacking, and nvolving a polar group interacting with a π-surface such as halogen-π, chalcogen-π, and carbonyl-π. (3) Nonpolar groups form weak but measurable stabilizing interactions with aromatic surfaces in organic solvents due to favorable dispersion and/or solvophobic effects. A good predictor of the interaction strength is provided by the change in solvent-accessible surface area. (4) Solvent effects modulate the aromatic interactions in the forms of solvophobic effects and competitive solvation, which can be modeled using solvent cohesion density and specific solvent-solute interactions.

microRNAs (miRNAs) can play a role in a variety of physiological and pathological regulation, and its role is achieved by regulating the expression of target genes. Our previous high-throughput sequencing found that ssc-miR-185 plays an important regulatory role in piglet diarrhea, but its specific target genes and functions in intestinal porcine epithelial cell (IPEC-J2) are still unclear. We intended to verify the target relationship between porcine miR-185 and cell division cycle 42 (CDC42) gene in IPEC-J2, and explored the effect of miR-185 on the proliferation of IPEC-J2 cells.

The TargetScan, miRDB and miRanda softwares were used to predict the target genes of porcine miR-185, and CDC42 was selected as a candidate target gene. The CDC42-3'UTR-wild type (WT) and CDC42-3'UTR-mutant type (MUT) segments were successfully cloned into pmirGLO luciferase vector, and the luciferase activity was detected after co-transfection with miR-185 mimics and CDC42-3'UTR. The expression level of CDC42 was analyzed usi185 can directly target CDC42 and promote the proliferation of IPEC-J2 cells. However, the detailed regulatory mechanism of miR-185/CDC42 axis in piglets resistance to diarrhea is yet to be further investigation.

Follicle selection is an important process in chicken egg laying. find more Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear .

In this study, the whole transcriptome sequencing of SY follicles with different expression level of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by qPCR. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles.

Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosall yellow follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection.

Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that CTRP3 participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture and identify the goose SMSCs in vitro. Meanwhile, to explore the function of CTRP3 in goose SMSCs.

Goose SMSCs were isolated using 0.25% trypsin from leg muscle of 15-20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium (DM) with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 were used to identify goose SMSCs. Quantitative real-time polymerase chain reaction and western blot were appplied to explore developmental expression a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

To determine the effect of postpartum PGF2alpha administration on colostrum and milk yield, colostrum IgG and piglet growth performance.

In total, 36 sows were included in the experiment. The sows were classified into two groups i) control (n=11) and ii) PGF2alpha (n=25). Sows in the PGF2alpha group received 10 mg of PGF2alpha within an hour after farrowing. The body weight of piglets was measured at 0 and 24 h after birth to estimate colostrum consumption. Colostrum was collected at 1 and 24 h after farrowing to determine IgG concentrations. For milk yield study, the remaining sows in the PGF2alpha group (n=23) were divided into two subgroups i) single PGF2alpha (n=12) and ii) multiple PGF2alpha (n=11). In the multiple PGF2alpha, the sows received repeated doses of PGF2alpha at seven and 14 days postpartum. The piglets' body weight was measured at 0, 1, 5 and 20 days of age. The milk yield of the sows was calculated.

Colostrum yield of sows averaged 5.62±2.25 kg. Sows treated with PGF2alpha postpartum ld and increased litter weight of piglets in primiparous sows.

Tibetan pigs, predominantly originating from the Tibetan Plateau, have been subjected to long-term natural selection in an extreme environment. To characterize the metabolic adaptations to hypoxic conditions, transcriptomic and proteomic expression patterns in the livers of Tibetan and Yorkshire pigs were compared.

RNA and protein were extracted from liver tissue of Tibetan and Yorkshire pigs (n = 3, each). Differentially expressed genes and proteins were subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analyses.

In the RNA-Seq and iTRAQ (Isobaric tags for relative and absolute quantitation) analyses, a total of 18,791 genes and 3390 proteins were detected and compared. Of these, 273 and 257 differentially expressed genes and proteins were identified. Evidence from functional enrichment analysis showed that many genes were involved in metabolic processes. The combined transcriptomic and proteomic analyses revealed that small molecular biosynthesis, metabolic processes, and organic hydroxyl compound metabolic processes were the major processes operating differently in the two breeds. The important genes include RDH16, APRT, PCYOX1, SORBS2, ENSSSCG00000036224, PLIN2, LAD1, KYAT1, and DDAH1.

The findings of this study provide novel insight into the high-altitude metabolic adaptation of Tibetan pigs.

The findings of this study provide novel insight into the high-altitude metabolic adaptation of Tibetan pigs.

This study was focused on the estimation of parameters of Wood's model and description of the lactation curve using the cows which were lactated over 24 months on the first lactation.

The database included 1 333 pure-bred dairy Simmental primiparous cows which lactated for 24 months (732 days). The initial dataset entering the procedure of assessment of parameters of Wood's function included 35 826 milk yield records. Milk yield was recorded throughout lactation, with the earliest record taken on day 6 and the latest on day 1 348 of lactation. This dataset was used for the assessment of parameters a, b, c of Wood's model using the non-linear statistical procedure. These parameters were estimated for different length of lactation. The assessed parameters were used for calculation of some characteristics of lactation curves.

The lowest value of a parameter (15.2317) of Wood's model of lactation curve was found out in lactations up to 305 days long, contrary to b and c parameters which were highest in those lactations (0.