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Larvae mortality post-stress was registered to 6, 12, and 18 mg L-1 of atrazine, and was more critical at 24 h. The increased doses of atrazine used in LC50-96h test depressed the development of Nile tilapia larvae. Fish submitted to stress (air exposition) and exposed to sub-lethal doses of atrazine showed significant mortality, indicating that stressors may increase the toxic effect of atrazine for Nile tilapia larvae. On the other hand, based on risk assessment atrazine can be classified as herbicide with low toxicity for Nile tilapia larvae and low toxicological risk.Phacidium lacerum (anamorph Ceuthospora pinastri) is a recently reported quarantine fungal pathogen responsible for postharvest rot in apples and pears. Very little is known about its pathogenicity, epidemiology, and best management practices. We screened pathogenicity of P. lacerum on twigs from seven and fruit from nine major commercial apple cultivars. Among the nine cultivars tested, detached fruit of Honeycrisp and Gala cultivars were the most susceptible, whereas WA38 (Cosmic Crisp) was the least susceptible (P less then 0.05). Effective concentrations to inhibit 50% growth (EC50) were determined in 41 baseline P. lacerum isolates. The mean EC50 values for four postharvest fungicides, i.e., fludioxonil (FDL), difenoconazole (DIF), thiabendazole (TBZ), and pyrimethanil (PYRI) were 0.16, 0.38, 0.54, and 0.72 µg/ml, respectively. The mean EC50 values for four preharvest fungicides, i.e., pyraclostrobin (PYRA), fluxapyroxad (FLUX), boscalid (BOSC), and fluopyram (FLUP) were 0.96, 12.64, 16.54, and 44.46 µg/ml, respectively. In situ efficacy trials were conducted on detached Gala apples treated preventively and curatively with the aforementioned fungicides. After 6 months of storage at 1°C, FDL and DIF provided full control followed by TBZ and PYRI, whereas the other preharvest fungicides provided fair or low efficacies. Findings of this study shed light on pathogenicity of this emerging pathogen and provide necessary knowledge for effective management of Phacidium rot.Stressful events are often vividly remembered. Although generally adaptive to survival, this emotional-memory enhancement may contribute to stress-related disorders. We tested here whether the enhanced memory for stressful events is due to the expectancy violation evoked by these events. Ninety-four men and women underwent a stressful or control episode. Critically, to manipulate the degree of expectancy violation, we gave participants either detailed or minimal information about the stressor. Although the subjective and hormonal stress responses were comparable in informed and uninformed participants, prior information about the stressor abolished the memory advantage for core features of the stressful event, tested 7 days later. Using functional near-infrared spectroscopy, we further linked the expectancy violation and memory formation under stress to the inferior temporal cortex. These data are the first to show that detailed information about an upcoming stressor and, by implication, a reduced expectancy violation attenuates the memory for stressful events.Cytokeratin (CK) 18 is an intermediate filament protein that plays a major functional role in the integrity and mechanical stability of cells. Since both CK8 and CK18 are major components of simple epithelia, in the context of tumors, they are expressed in most carcinomas, and have been studied as diagnostic and prognostic markers in tumor pathology. CK18 is also cleaved by some caspases during apoptosis. Three-dimensional (3D)-cultured cancer cells are useful for cancer research as an intermediate model between in vitro cancer cell line cultures and in vivo tumors. In this study, we produced rat monoclonal antibodies (mAbs) through immunization of the lysate from 3D-cultured DLD-1 cells to elucidate a characteristic feature of a tumor, and our results showed that mAb 2H7 recognized human CK18. Furthermore, we indicated that mAb 2H7 was useful for immunoblotting, immunoprecipitation, and immunofluorescence staining. Therefore, it may be useful as a diagnostic tool for evaluating malignancy.Fusarium temperatum (Scaufl. & Munaut) is one of the most important fungal pathogens that cause ear and stalk rots in maize. In this study, we sequenced genomes of two F. temperatum isolates (KFI615 and KFI660) isolated from corn ears in Poland. A total of 110.3 and 116.3 million 100-nucleotide paired-end clean reads were obtained for KFI615 and KFI660, which were assembled into 20 and 18 scaffolds with an estimated genome size of 45.21 and 45.00 Mb, respectively. These genome sequences provide important resources for understanding pathogenicity and biology of the pathogens within the Fusarium fujikuroi complex.[Formula see text] Copyright © 2021 The Author(s). learn more This is an open access article distributed under the CC BY-NC-ND 4.0 International license.AbstractSpite is the most surprising prediction of inclusive fitness theory because it suggests that a gene can be favored by natural selection despite causing harm to both the individuals that carry it and those around them. A gene for spite can only be favored because of negative relatedness, which means that the actor that carries the gene is less likely to share the gene for spite with the surrounding recipients than the random expectation. While positive relatedness can be simply reduced to the intuitive concept of kinship, negative relatedness is deeply counterintuitive. Here I clarify that negative relatedness is frequency dependent, and I identify a hidden assumption in its widely used formula. Accordingly, while the well-studied "lighter" side of inclusive fitness (with helping behaviors and positive relatedness) is dominated by traits that are favored under kin selection, I predict that the understudied "darker" side of inclusive fitness (with harming behaviors and negative relatedness) is dominated by traits that are favored under greenbeard/kind selection-and I discuss the existing evidence that tentatively supports this hypothesis.AbstractWhen populations evolve adaptive reaction norms in response to novel environments, it can occur through a process termed genetic accommodation. Under this model, the initial response to the environment is widely variable between genotypes as a result of cryptic genetic variation, which is then refined by selection to a single adaptive response. Here, I empirically test these predictions from genetic accommodation by measuring reaction norms in individual genotypes and across several time points. I compare two species of Drosophila that differ in their adaptation to ethanol (D. melanogaster and D. simulans). Both species are human commensals with a recent cosmopolitan expansion, but only D. melanogaster is adapted to ethanol exposure. Using gene expression as a phenotype and an approach that combines information about expression and alternative splicing, I find that D. simulans exhibits cryptic genetic variation in the response to ethanol, while D. melanogaster has almost no genotype-specific variation in reaction norm.