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Hair loss transplant of Man Activated Pluripotent Base Cell-Derived Cardiomyocytes in a Mouse Myocardial Infarction Style.

Forkhead box M1 (FOXM1), a nuclear transcription factor which activates cell cycle regulatory genes, is highly expressed in a majority of human cancers. The function of FOXM1 independent of nuclear transcription is unknown. In the present study, we found the FOXM1 protein inside of the mitochondria. Using site-directed mutagenesis, we generated FOXM1 mutant proteins that localized to distinct cellular compartments, uncoupling the nuclear and mitochondrial functions of FOXM1. Directing FOXM1 into the mitochondria decreased mitochondrial mass, membrane potential, respiration and electron transport chain (ETC) activity. In mitochondria, the FOXM1 directly bound to and increased the pentatricopeptide repeat domain 1 (PTCD1) protein, a mitochondrial leucine-specific tRNA binding protein that inhibits leucine-rich ETC complexes. Mitochondrial FOXM1 did not change cellular proliferation. Thus, FOXM1 translocates into mitochondria and inhibits mitochondrial respiration by increasing PTCD1. We identify a new paradigm that FOXM1 regulates mitochondrial homeostasis in a process independent of nuclear transcription.Exposure modeling is a valuable tool for assessing chemical vapor exposures that occur during transient events such as small spills of volatile liquids. Models are available to estimate liquid evaporation rates and resulting air concentrations. However, liquid evaporation rate models require the surface area of the puddle in order to provide vapor generation rates in terms of mass per time. This study developed an approach to model the surface area of small spills of pure liquids. A theoretical equation exists relating puddle depth to a liquid's surface tension, density, and contact angle. A contact angle is a characteristic of liquid-solid interactions at the edge of a puddle. If the depth of a puddle can be calculated and the volume of the liquid spilled is known, the surface area of the puddle can be determined. Values for density and surface tension are published. see more Contact angles, however, are not readily available. Five hundred and eighty experimental spills were conducted using acetone, ethanol and water. The effective contact angle for each spill was determined. Spill volumes varied from 1.0-30.0 mL. see more The height of the liquid release varied from 0-15 cm onto a variety of surfaces. The effective contact angle of a puddle was most strongly associated with the liquid's polarity. The height of the liquid release and type of surface had significant, but smaller effects on the puddle size. The effective contact angle of a puddle from a spill can be estimated as ln(ϴeff) = 3.73 - 1.17 · 1χυ/f - 0.06 · h + S. In this equation, 1χυ/f is the polarity index of the liquid, h is the height of liquid release (cm), and S is a surface constant. ϴeff can be used with the liquid density, surface tension and volume to calculate the surface area of the puddle. The surface area of the puddle can then be used in evaporation rate models to determine a vapor generation rate for input to vapor concentration models.Lymphocyte recirculation within the human body is essential for efficient pathogen detection and immune responses. So far, immune cell migration has been investigated largely using ovine and murine models, with little evidence in humans. Here, we analyzed peripheral blood of healthy individuals following primary vaccination with the Yellow Fever vaccine YF-17D. We found that the number of leukocytes was transiently and sharply reduced in blood as detected on day 7 after vaccine administration. The T cell drop was restricted to cells expressing the lymph node-homing chemokine receptor CCR7. Interestingly, the vaccine-induced drop positively correlated with the expression of CD69 by the T cells before vaccination. This suggests that CCR7+ T cells are being trapped within the lymph nodes through CD69-induced suppression of egress. Strikingly, we further found that the T cell drop negatively correlated with CD8 T cell activation and with production of neutralizing antibodies. In conclusion, early and transient T cell depletion in blood negatively correlated with protective immune response events induced by YF-17D vaccination. Our data highlight baseline CD69 expression and early drop in T cells as potential biomarkers of the Yellow Fever vaccine response.Eye movement-based human-computer interactions are emerging in diverse scenarios. When selecting targets on a user interface, the method of combining fast gaze pointing with reliable manual action is becoming increasingly popular. However, this method suffers from noise in gaze pointing caused by eye jitters and users' habitual early move-away of gaze before manual actions. Here we propose a novel solution to mitigate these problems by locking the gaze cursor at the target for imminent manual selection. We compared this gaze-lock cursor with a conventional gaze cursor in a typing task with varying key sizes and key gaps. Results show that typing performance was significantly better with larger key size and gap. More importantly, the gaze-lock cursor significantly increased speed and decreased errors when compared to a conventional gaze cursor. Our findings demonstrate that the gaze-lock cursor is a promising tool for gaze interactions involving frequent target selections. Practitioner summary Target selection by gaze pointing and manual confirmation suffers from eye jitters and users' habitual early move-away of gaze before manual actions. The performance of this method can be improved by applying the gaze-lock cursor we proposed, increasing target size or increasing the target gap. Abbreviations WTC warping to target center; ALCM automatic lock of cursor movement; LCD liquid crystal display; EWMA exponential weighted moving average; ER error rate; ET execution time; ED edit distance; CV coefficients of variation; ANOVA analysis of variance; GUIs general user interfaces.Background The May 2017 publication of the updated Associated Press (AP) Stylebook offered guidance that advised against stigmatizing. The objective of this study was to assess the frequency of stigmatizing terms describing substance use and disorder in news articles before and after the update of the AP Stylebook. Methods We reviewed articles containing terms "opioid" or "addiction" from three major news outlets. We counted the number of AP Stylebook proscribed terms found in each article and compared the proportions of articles from each outlet with proscribed terms before and after AP Stylebook publication. Results In 2016, 56-94% of articles from each of the three news outlets contained a proscribed term. The use of proscribed terms in articles identified by searching "opioid" published in the New York Times decreased (72% vs. 94%, p = 0.01) after the AP Stylebook change. For other news outlets, there were no significant differences, though all proportions were lower after publication. Conclusions Evidence for a decrease in the use of stigmatizing terminology for substance use and disorders in news articles after publication of guidance was limited.