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Researchers now have a new avenue to investigate when it comes to miRNA-based therapeutics. miRNAs have the potential to be valuable biomarkers for disease detection. Variations in miRNA levels may be able to predict changes in normal physiological processes. At the epigenetic level, miRNA has been identified as a promising candidate for distinguishing and treating various diseases and defects.
In recent pharmacology,plantsmiRNA-based drugs have demonstrated a potential role in drug therapeutics. The purpose of this review paper is to discuss miRNA-based therapeutics, the role of miRNA in pharmacoepigenetics modulations, plant miRNA inter-kingdom regulation, and the therapeutic value and application of plant miRNA for cross-kingdom approaches. Target prediction and complementarity with host genes, as well as cross-kingdom gene interactions with plant miRNAs, are also revealed by bioinformatics research. We also show how plant miRNA can be transmitted from one species to another by crossing kingdom boundaries in this review. Despite several unidentified barriers to plant miRNA cross-transfer, plant miRNA-based gene regulation in trans-kingdom gene regulation may soon be valued as a possible approach in plant-based drug therapeutics.
This review summarised the biochemical synthesis of miRNAs, pharmacoepigenetics, drug therapeutics and miRNA transkingdom transfer.
This review summarised the biochemical synthesis of miRNAs, pharmacoepigenetics, drug therapeutics and miRNA transkingdom transfer.
This study aimed to evaluate the effects of root canal irrigation on maximum operative torque and vertical force of Wave One Gold Primary (WOG), Reciproc Blue R25 (RB) and Protaper Next X2 (PTN) endodontic rotary instruments during simulated root canal preparation in the presence and absence of a surfactant "benzalkonium chloride".
A custom-made automated irrigation and torque/force analyzing device connected with endomotor was used for the study. Acrylic resin blocks with simulated curved root canals of 35° were randomly assigned to 6 groups for each file. The following irrigants were used during simulated root canal preparation with continuous rotation or reciprocation motion with pecking mode 5% NaOCl and 5% NaOCl with 0.84% benzalkonium chloride. Maximum torque and vertical force values of files were recorded during preparation.
BAC addition to NaOCl did not significantly affect maximum torque values of instruments (p>0.05). WOG generated lowest torque value compared with PTN and RCB in both groups (p<0. 05). RB generated higher vertical force compared with WOG and PTN instruments in NaOCl+BAC group. The surface modifier decreased the maximum force value of WOG instrument (p<0.05).
Surfactan addition to NaOCl had no negative effects on maximum torque and force of the instruments.
Surfactan addition to NaOCl had no negative effects on maximum torque and force of the instruments.
Giant cell lesions of the jaws (GCLJ) may rarely occur in the setting of RASopathy syndromes such as Noonan syndrome or neurofibromatosis I. Recently, central giant cell granulomas (CGCG), the most common of the GCLJ, have been recognized as benign neoplasms characterized by Ras/MAPK signaling pathway mutations. This provides a rational basis for understanding GCLJ in RASopathy syndromes as syndromically occurring CGCG. This review aims to summarize the clinicopathologic features of syndromic CGCG and to review the salient clinical and craniofacial features of the syndromes in which they may rarely occur.
An electronic search in 3 databases was performed, looking for GCLJ/CGCG in RASopathy syndromes.
124 CGCG in 56 patients were identified across 6 RASopathy syndromes. Median age at syndromic CGCG diagnosis is 11 years; 69.6% (39/56) patients developed two or more CGCG; 58.9% (33/56) presented with bilateral posterior mandibular CGCGs, mimicking cherubism. Of 88 CGCG with follow-up, 22.4% (13/58) of excised/resected CGCG recurred while 46.7% (14/30) of monitored CGCG showed continued growth.
Syndromic CGCG involves multiple RASopathy syndromes and may mimic cherubism or, when solitary, sporadically occurring CGCG. Familiarity with other clinical findings of RASopathy syndromes is critical for appropriate diagnosis and patient management.
Syndromic CGCG involves multiple RASopathy syndromes and may mimic cherubism or, when solitary, sporadically occurring CGCG. Familiarity with other clinical findings of RASopathy syndromes is critical for appropriate diagnosis and patient management.Correct implant positioning poses a major challenge in modern dentistry and mismatch between the planned and final implant position is one of the most common treatment complications. A surgical guide or a template is a transmission device which enables the implant placement in the intended position as accurate as possible. Case study A woman aged 60 came into dental office to resolve a single tooth loss in regions 15 and 24. Radiographic analysis and clinical examination showed a lack of transversal space in the regions of implantation. Considering the fact of having a narrow area available for implantation, a computer-guided implant therapy using the M-Guide system was selected. The treatment plan was a prosthetic restoration involving the placement of two implants in areas 15 and 24 and the fabrication of zirconium oxide crowns. After digital planning, a fully guided surgical protocol was performed. Immediately after implantation, a temporary suprastructure and temporary crowns were placed. After a period of osseointegration, a definitive prosthetic restoration was made.
The aim of this retrospective study was to determine the frequency of hypodontia, hyperdontia, invagination, impaction, dilacerations, peg-shaped lateral incisors, taurodontism and short or blunt and narrow or pipette-shaped roots in Croatian orthodontic patients.
506 orthopantomographs and study casts from 12-16 year-old orthodontic patients treated at the Department of Orthodontics, School of Dental Medicine University of Zagreb were analyzed.
At least one dental anomaly was present in 24.1% of patients, and more than anomaly in 1.2% of them. Inflammation inhibitor The frequency was not significantly different between genders. Hypodontia was the most frequent anomaly with the incidence of 7.5%, followed by teeth impaction with the incidence of 6.3%.
The distribution and the prevalence of anomalies were similar to those described in the general Croatian population.
The distribution and the prevalence of anomalies were similar to those described in the general Croatian population.
Dentin thickness in concave areas of the root creates risk for complications such as strip perforation during endodontic treatment. The study aims to examine dentin thickness of the danger (DZ) and safety zone (SZ), canal configuration, and the presence of isthmus in the mesiobuccal root of maxillary molars.
Cone-beam Computed Tomography (CBCT) images of 1251 teeth belonging to 642 patients were retrospectively reviewed. The dentin thicknesses at DZ and SZ in maxillary molars with one (MB) or two mesiobuccal canals (MB1, MB2) were measured at the 3 mm apical to the furcation level. Vertucci's canal configurations and the isthmus rate were recorded. The Chi-square test andThe Student's t-test were performed.
MB2 rate was higher in maxillary first molars (61.68%) than second molars (39.36%). Isthmus rates were 27.3% and 44.11% in first and second molars. DZ thickness was thinner than the dentin thickness in the SZ in both first and second molars with one or two mesial canals (p< 0.05). In teeth with single canal, the mean DZ thickness was 0.88mm. In teeth with two canals, the mean DZ thicknesses were 0.83mm and 0.80mm for MB1 and MB2 canals, respectively.
MB2 rate was higher in the first molar (61.68%), and the isthmus rate was higher in the second molar (44.11%). DZ and SZ were thinner in MB2 than in MB1 at the maxillary molars with two mesial canals. The results indicated that more conservative preparation must be applied to the MB2 canal in the maxillary molars.
MB2 rate was higher in the first molar (61.68%), and the isthmus rate was higher in the second molar (44.11%). DZ and SZ were thinner in MB2 than in MB1 at the maxillary molars with two mesial canals. The results indicated that more conservative preparation must be applied to the MB2 canal in the maxillary molars.
The study aimed to compare the color stability of two different light-cured composites after immersion in three liquids and the effectiveness of 16% carbamide peroxide (CP) in removing the discoloration.
Color stability of a microhybrid (Z250, 3M ESPE) and nanocomposite (Z550, 3M ESPE) was evaluated after immersion in instant coffee, tea, Coca-Cola, and deionized water as a control group (n=5). Samples were kept in liquids for four hours daily at 37°C for 30 days. Furthermore, 16% CP was applied for the following 14 days, simulating night whitening. A digital spectrophotometer was used for color measurement based on the CIEL*a*b* color coordinates. The color changes (∆E) were measured at baseline, after immersion in the beverages, and also after the teeth whitening procedure. Mixed and factorial ANOVA followed by Bonferroni's post-hoc test were used for statistical evaluation (p≤0.05).
Tested resin composites showed a color change over the acceptability threshold (ΔE*> 3.48) after immersion in coffee and tea. Nanocomposite reported a significant increase in discoloration in coffee after 30 days (p <0.05). The color of both materials significantly changed (p<0.05) along all three L*a*b* axes in coffee and tea to darker, yellow, and red. Whitening with 16% CP was effective in removing external discoloration in both examined composite materials.
Coffee and tea induced clinically detectable color changes in dental composites tested, with cumulative effects. Whitening represents an efficient method for the removal of surface discoloration in composite restorations.
Coffee and tea induced clinically detectable color changes in dental composites tested, with cumulative effects. Whitening represents an efficient method for the removal of surface discoloration in composite restorations.
Caries has a harmful impact on oral and general health and is a major public health problem among children and adolescents. The objective of present study was to investigate into dental caries, oral hygiene, the frequency of brushing habits and dental visits among 15- year- old adolescents.
This cross-sectional study was carried out on a random sample of 323 15- year- old adolescents in different schools and municipalities of Kosovo. Oral clinical examination and self-administered questionnaire were used to obtain information about dental caries and oral health practices. Oral hygiene and caries status in permanent dentition was assessed through the DMFT index and Oral Hygiene Index - Simplified (OHI-S). The level of statistical significance was set at p<0.05.
The total mean of the DMFT index was 3.21 ± 2.193, while component D of the DMFT index dominated in both genders, with slightly higher values in boys compared to girls (2.15±2.092, and 1.91±1.919). The mean OHI-S index of adolescents aged 15 was found to be 1.
