Kragelundspears3218

© 2020 Published by Elsevier Ltd.This paper determines the effect of steel, glass, and nylon fibers on the compressive strength and ultrasonic pulse velocity (UPV) of fiber reinforced concrete. The influence of different fiber types, fiber volume fraction, and water to cement ratios on the compressive strength of fiber reinforced concrete was tested using the compression test machine (CTM) and ultrasonic pulse velocity tester. Experiments were carried out at different ages on more than 100 cylindrical specimens. A comparison between the experimental results and equations available in the literature for prediction of compressive strength in terms of UPV was conducted to better evaluate the accuracy of available methods, when the type and volume fraction of fibers change. A new empirical equation that accounts for the presence of different types of fibers and fiber volume fraction is proposed to better estimate the compressive strength of steel, glass, and nylon fiber reinforced concrete. © 2020 The Authors.In the tick life cycle, embryogenesis is the only stage of development wherein no blood meal is required. Nevertheless, even in the absence of a blood meal, which is the source of nutrients as well as the ferrous iron and heme that could cause oxidative stress in ticks, malondialdehyde (MDA) has been reported to increase during this period. Additionally, the knockdown of some oxidative stress-related molecules such as ferritin has resulted in abnormal eggs and embryonic death. Here, we investigate the gene and protein expression profiles of the identified glutathione S-transferases (GSTs) and ferritins (Fers) of the tick H. longicornis during embryogenesis through quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blotting, respectively. We also confirm the lipid peroxidation and ferrous iron concentration level using a thiobarbituric acid reactive substances (TBARS) assay. Finally, we attempt to correlate these findings with the events occurring by establishing a staging process in H. longicornis embryos. Lipid peroxidation increased during the course of embryogenesis, as does the amount of GST proteins. On the other hand, the GST genes have high expression at the 1st day post-oviposition, during the early stage of embryogenesis and at day 10 during the period wherein the germ band is observable. Fer gene expression also starts to increase at day 10 and peaks at day 15. In the ferritin proteins, only the secretory ferritin (Fer2) is detected and constitutively expressed during embryogenesis. Events occurring during embryogenesis, such as energy production and iron metabolism for cellular proliferation and differentiation cause oxidative stress in the embryo. To counteract oxidative stress, it is possible that the embryo may utilize oxidative stress-related molecules such as GSTs and Fer2, which could be either maternally or embryo-derived. © 2020 The Author(s).This paper summarizes experimental findings of the regenerative capability of nitrogen on Fuller's earth. Regenerating new oil from aged oil involves several cycles requiring a large volume of Fuller's earth. The use of large amounts of Fuller's earth is not economically profitable for companies that carry out regenerative operations to service transformer oils and can have environmental implications, since Fuller's earth is usually used to reclaim transformer oil in a one-time batch basis then disposed of. To improve the situation, a new technique to reduce the number of regeneration cycles is proposed. It is shown that Fuller's earth can be improved by purging it with continuous flowing of a fluid, preferably dry nitrogen. © 2020 The Author(s).A dataset of seventy-two (72) cytotoxic compounds of the National Cancer Institute (NCI) was studied by QSAR and docking approaches to gain deeper insights into ligands selectivity on SK-MEL-2 cell line. The QSAR model was built using fifty (50) molecules and the best-generated model based on multiple linear regression showed, respectively good quality of fits ( R 2 (0.864), R a d j u s t e d 2 (0.845), Q2 cv (0.799) and R p r e d 2 (0.706)). The model's predictive ability was determined by a test set of twenty-two (22) compounds. Compounds 30 and 41 were selected as templates for in silico design because they had high pGI50 activity and are in the model's applicability domain. The obtained information from the model was explored to design novel molecules by introducing various modifications. Moreover, the designed compounds with better-predicted activity (pGI50) values were selected and docked on the active site of the protein (PDB-CODE 3OG7) which is responsible for melanoma cancer to elucidate their binding mode. AN2 (-12.1kcalmol-1) and AC4 (-12.4kcalmol-1) showed a better binding score for the target when compared with (vemurafenib, -11.3kcalmol-1) the known inhibitor of the target (V600E-BRAF). selleck compound These findings may be very helpful in early anti-cancer drug development. © 2020 The Author(s).Mycobacterium tuberculosis has instigated a serious challenge toward the effective treatment of tuberculosis. The reoccurrence of the resistant strains of the disease to accessible drugs/medications has mandate for the development of more effective anti-tubercular agents with efficient activities. Time expended and costs in discovering and synthesizing new hypothetical drugs with improved biological activity have been a major challenge toward the treatment of multi-drug resistance strain M. tuberculosis (TB). Meanwhile, to solve the problem stated, a new approach i.e. QSAR which establish connection between novel drugs with a better biological against M. tuberculosis is adopted. The anti-tubercular model established in this study to forecast the biological activities of some anti-tubercular compounds selected and to design new hypothetical drugs is subjective to the molecular descriptors; AATS7s, VE2_Dzi, SpMin7-Bhe and RDF110i. The significant of the model were observed with R2 of 0.8738, R2 adj of 0.8351 Q_sis a novel drug candidate against the tuberculosis. Moreover, in-vitro and in-vivo test could be carried out to validate the computational results. link2 © 2020 The Author(s).Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has been traditionally used for curing different ailments. The purpose of present study was to investigate the antioxidant activities of fruits of L. cubeba using different solvent extracts, quantification of phenolics, toxicity studies and DNA damage protective activities. The antioxidant activities of fruits using five different solvent extracts completed utilizing different in vitro examines. The quantitation of phenolic and polyphenolic compounds in the methanol extract of the fruits was carried out by HPLC. The in vitro haemolytic examination of plant concentrates were completed on rat erythrocytes. Appraisal of cytotoxicity of eatable fruits was assessed by MTT measure. The genotoxicity of the contemplated plant was tried by the single-cell gel electrophoresis comet measure. The DNA defensive impacts of the aqueous extracts of fruits on rodent lymphocyte DNA lesions were likewise assessed with the comet test. The extract obtained by methanol exhibited the highest antioxidant activity. The HPLC examination of the methanol concentrate of the plant demonstrated the occurrence of different phenolic acids and flavonoids like caffeic acid (145.96μg/100mg DE), syringic acid (125.85 μg/100mg DE), ferulic acid (155.89 μg/100mg DE), apigenin (28.43 μg/100mg DE), kaempferol (53.41 μg/100mg DE) etc. in various amounts. The consequences of haemolytic lethality, cytotoxicity and genotoxicity of fluid concentrates of the edible plant ensure the security at cell and genomic level. The fluid concentrate of the plant fundamentally repressed DNA harm and these information recommend that the watery concentrate of L. cubeba can forestall oxidative DNA harm to rodent lymphocytes, which is likely because of antioxidant constituents in the concentrate. These outcomes demonstrate that L. cubeba can be utilized in dietary applications with a possibility to diminish oxidative pressure. © 2020 The Authors.This research aims to define the depth of Moho in Iran by collocation method using gravimetric data with seismic information. The definition of the Moho in the Iranian region is of considerable importance due to the geological complexity of the area also characterized by tectonic and orogenic events of particular uniqueness. We applied the collocation method to Moho recovery using the gravity data generated by GOCO03S model reduced by topography/bathymetry, sediment and consolidated crust effects from CRUST1.0. These data have been complemented with seismic Moho depth estimates. A compilation of 213-points seismic depth has been collected over Iran and used in the integrated gravimetric-seismic inversion. link3 Among them, 140 seismic points have been selected completely random and included as data in the integrated collocation approach for Moho depth estimation. The 73 remaining seismic points have been used as checking points for validating the estimated Moho. In the first run, gravity data only have been considered to collocation Moho recovery. When comparing this gravimetric solution with the 73 seismic checking points, a standard deviation of 6.2 km was found. In case of considering the regional seismic depths into the collocation approach, the standard deviation of the residuals between our results and seismic checking Moho depths improved to 4.9 km. It must be stated that, even in the integrated inversion, a significant discrepancy between the seismic and the integrated gravimetric-seismic Moho is present in the South Caspian Basin. Low quality of CRUST1.0 could explain this inconsistency in this area. © 2020 The Author(s).Lysosomal storage diseases (LSDs) are a group of about 50 inborn errors of metabolism characterized by the lysosomal accumulation of partially or non-degraded molecules due to mutations in proteins involved in the degradation of macromolecules, transport, lysosomal biogenesis or modulators of lysosomal environment. Significant advances have been achieved in the diagnosis, management, and treatment of LSDs patients. In terms of approved therapies, these include enzyme replacement therapy (ERT), substrate reduction therapy, hematopoietic stem cell transplantation, and pharmacological chaperone therapy. In this review, we summarize the Colombian experience in LSDs thorough the evidence published. We identified 113 articles published between 1995 and 2019 that included Colombian researchers or physicians, and which were mainly focused in Mucopolysaccharidoses, Pompe disease, Gaucher disease, Fabry disease, and Tay-Sachs and Sandhoff diseases. Most of these articles focused on basic research, clinical cases, and mutation reports. Noteworthy, implementation of the enzyme assay in dried blood samples, led to a 5-fold increase in the identification of LSD patients, suggesting that these disorders still remain undiagnosed in the country. We consider that the information presented in this review will contribute to the knowledge of a broad spectrum of LSDs in Colombia and will also contribute to the development of public policies and the identification of research opportunities. © 2020 The Author(s).