Riddlepearce4327

[This corrects the article DOI 10.1021/acsomega.0c02445.].[This corrects the article DOI 10.1021/acsomega.0c01443.].

The main objective of this study was to investigate the antithrombotic and antiplatelet effect of the extract from

(L.) Nees and understand the mechanisms by which it exerts its antithrombotic and antiplatelet mechanisms.

The antithrombotic effective parts (RPE) were isolated using D101 macroporous adsorption resin and potential active ingredients (JAC) were isolated using the preparative liquid-phase method. The lactate dehydrogenase kit was used to determine the toxicity of RPE and JAC to platelets. The antiadhesion effect of RPE and JAC on platelets was observed by fluorescence microscopy with rhodamine phalloidin. Antithrombotic efficacy of RPE and JAC in vivo was evaluated by establishing a rat tail thrombosis model. Contents of p-selectin, TXB

, and 6-keto-PGF

in rat serum were measured using an enzyme-linked immunosorbent (ELISA) assay, and the rat black tail rate was measured to prove the protective effect of RPE and JAC on the tail thrombus rat model. Western blot was used for detection of Its mechanism may be via preventing integrin αIIbβ3 activation, which in turn leads to the inhibition of the phosphorylation of the MAPK family and further suppresses TXA2, which leads to the antithrombotic and antiplatelet effects.To meet the technical requirements of deep fluid diversion in Bohai oilfield, the swelling property, plugging effect, transport characteristics of polymer microspheres, and fluid diversion effect in heterogeneous cores are studied in this paper. There are two kinds of polymer microspheres including core-shell microspheres and traditional microspheres. The instruments used in this study include a biomicroscope, a metallurgical microscope, a scanning electron microscope, and core displacement experimental devices. The results of microscopes indicated that the core-shell microspheres were successfully synthesized, and the microspheres had good hydration expansion effect. The expanded microspheres could attract each other through the electrostatic force of anions and cations to achieve the purpose of coalescence. Compared with traditional microspheres (initial particle size is 3.8 μm), the initial particle size of the synthesized core-shell microspheres is close to 3.3 μm, but the particle size distribution is more concentrated, so the injection performance is close to that of traditional microspheres (initial particle size is 3.8 μm). After 8 days of hydration expansion, although the expansion multiple is small, it can coalesce and enhance the plugging effect, which can adapt to a wider range of permeability, ranging from 200 × 10-3 to 3000 × 10-3 μm2 (200 × 10-3-1500 × 10-3 μm2 for traditional microspheres). Under the same conditions (heterogeneous core), compared with the traditional microspheres, the core-shell microspheres have the characteristics of coalescence. Therefore, its fluid diversion effect is better, and the oil recovery is increased by 5.5%. Namodenoson datasheet Nevertheless, there is the "end effect" during the injection process, which weakens the steering effect of deep liquid flow. The results show that the "end effect" can be effectively reduced by alternate injection of microspheres and water. Meanwhile, the effect of deep fluid diversion is improved, and the increase of oil recovery is increased by 2.06%.To suppress catalytic coking, TiO2 passivation films were deposited on the inner walls of SS316 stainless steel tubes by atomic layer deposition (ALD). Indentation test results showed a platform on the indentation curve of TiO2 films grown over 2000 ALD cycles due to internal stress-induced microcracks. In coking experiments, the TiO2-coated tubes exhibited a higher heat flux and lower pressure difference than bare ones. Analysis of the coking surface revealed that TiO2 thin film passivation can reduce the size and number of particulate deposits. At the same time, passivation treatment inhibits the formation of filamentous carbon and improves anti-coking performance by reducing the ability of the tube to adsorb amorphous deposition products. The coking surfaces of TiO2-coated tubes had less graphitization, indicating that the coking products had fewer defects and lower activated carbon contents.The use of lightweight pozzolanic aggregates as partial replacement of cement results in low-density cement systems. Such systems ensure effective zonal isolation in zones where low equivalent circulating densities are required. However, low pozzolanic materials, such as fly ash and ground granulated blast furnace slag (GGBFS), have poor early-age strength development and long set times, especially when used in high volume, that is, exceeding 50% by weight of cement. The objective of this study is to develop a lightweight oil- and gas-well cement recipe with enhanced properties employing the synergism that exist among fly ash, GGBFS, and silica fume. The experimental work was per laboratory procedure outlined by American Petroleum Institute. Portland class G cement and the aluminosilicate materials were admixed in water to form a 13.5 ppg slurry. Chemical admixtures were used to facilitate the dissolution of reactive components in the pozzolanic materials and the hydration process. The experimental investigations were done at 150 °F and an ambient pressure of 1500 psi. The newly developed lightweight recipe exhibited excellent rheological and mechanical properties, having a wait-on-cement time for about 4 h and a 24 h sonic strength of 3116 psi, at 150 °F and 1500 psi. The thickening time was approximately 4 h (70 Bc). This slurry will be ideal in zones that would require a low hydrostatic slurry column and rapid gel strength development.We prepared novel flame retardants with concurrent excellent smoke-suppression properties based on lignin biomass modified by functional groups containing N and P. Each lignin-based flame retardant (Lig) was quantitatively added to a fixed amount of epoxy resin (EP), to make a Lig/EP composite. The best flame retardancy was achieved by a Lig-F/EP composite with elevated P content, achieving a V-0 rating of the UL-94 test and exhibiting excellent smoke suppression, with substantial reduction of total heat release and smoke production (by 46.6 and 53%, respectively). In this work, we characterized the flame retardants and the retardant/EP composites, evaluated their performances, and proposed the mechanisms of flame retardancy and smoke suppression. The charring layer of the combustion residual was analyzed using SEM and Raman spectroscopy to support the proposed mechanisms. Our work provides a feasible method for lignin modification and applications of new lignin-based flame retardants.Grinding, an essential procedure for size reduction and fresh surface exposure of mineral particles, plays an important role in mineral flotation. The grinding media are the key factors for effective grinding and thus for successful flotation. In this study, ceramic ball (CB) and cast iron ball (CIB), two representative grinding media, were chosen to investigate the effects and mechanisms of grinding media on the flotation behavior of scheelite. The results of pure scheelite flotation show that scheelite ground by CB has a better floatability than that ground by CIB. Scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and X-ray photoelectron spectroscopy (XPS) analyses indicate that there are Fe species, namely, elemental iron (Fe), ferrous oxide (FeO), and iron oxyhydroxide (FeOOH), coated on the surfaces of scheelite ground by CIB but not in the case of scheelite ground by CB. The dissolved oxygen (DO) and inductively coupled plasma-atomic emission spectroscopy (ICP-AES) tests show that Fe ions exist in the CIB grinding slurry but not in the case of CB grinding slurry. Compared with the CB grinding slurry, the CIB grinding slurry has a lower DO content and higher Ca ion concentration. Zeta potential results reveal that the Fe species in the CIB grinding reduce the NaOl adsorption on the scheelite surfaces. Finally, the deleterious effect of CIB grinding on the flotation behavior of scheelite is verified by the actual scheelite ore flotation experiments.To support clinical use, a multigram-scale process has been developed to provide 5-MeO-DMT, a psychedelic natural product found in the parotid gland secretions of the toad, Incilius alvarius. Several synthetic routes were initially explored, and the selected process featured an optimized Fischer indole reaction to 5-MeO-DMT freebase in high-yield, from which the 11 succinate salt was produced to provide 136 g of crystalline active pharmaceutical ingredient (API) with 99.86% peak area by high-performance liquid chromatography (HPLC) and a net yield of 49%. The report provides in-process monitoring, validated analytical methods, impurity formation and removal, and solid-state characterization of the API essential for subsequent clinical development.The biocatalysts responsible for the enzymatic synthesis of hydroxygenisteins, derivatives of genistein with multiple activities, usually show regioselective promiscuity, hydroxylating genistein to form a mixture of multiple products, which, in turn, results in a cumbersome separation and purification. Hence, it is highly desired to explore the underlying mechanism regulating the regioselectivity of hydroxylases. M13 is a variant of cytochrome P450 BM3 with oxidant activity toward genistein. Herein, genistein was demonstrated to be hydroxylated by M13 to form a mixture of 3'-hydroxygenistein (3'-OHG) and 8-hydroxygenistein (8-OHG), each giving 4% conversion with a ratio of 11. Protein engineering toward M13 was thus performed to improve its regioselectivity. When isoleucine at position 86 was mutated into cysteine, the resultant variant M13I86C displayed improved regioselectivity toward 3'-OHG with an increased conversion of 8.5%. The double mutation M13I86CP18W further boosted the conversion of 3'-OHG to 9.6%, and the ratio of 3'-OHG to 8-OHG increased to 121. Conversely, both CoCl2 and glucose 6-phosphate (G6P) could lead to more 8-OHG. When Co2+ reached 37.5 mM, M13I86CP18W could give an 8-OHG conversion of 22.4%. The maximal ratio of 8-OHG to 3'-OHG reached 130 when 62.5 mM Co2+ was included in the reaction mixture. With the increase of G6P from 10 to 40 mM, the conversion of M13I86CP18W to 8-OHG gradually increased to 22.6%, while the conversion to 3'-OHG decreased to 6%. Thus, both intrinsic residues and external reaction conditions can affect the regiospecificity of M13, which laid the foundation for the selection of suitable biocatalysts for the hydroxylation of genistein.Nanotubes have been considered as promising candidates for protein delivery purposes due to distinct features such as their large enough volume of cavity to encapsulate the protein, providing the sustain and target release. Moreover, possessing the properties of suitable cell viabilities, and biocompatibility on the wide range of cell lines as a result of structural stability, chemical inertness, and noncovalent wrapping ability, boron nitride nanotubes (BNNTs) have caught further attention as protein nanocarriers. However, to assess the encapsulation process of the protein into the BNNT, it is vital to comprehend the protein-BNNT interaction. In the present work, the self-insertion process of the protein SmtA, metallothionein, into the BNNT has been verified by means of the molecular dynamics (MD) simulation under NPT ensemble. It was revealed that the protein was self-inserted into the BNNT through the protein-BNNT van der Waals (vdW) interaction, which descended and reached the average value of -189.63 kcal·mol-1 at 15 ns of the simulation time.