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01). For related intentions, sleep relative to wake benefitted young adults' performance (p<0.001) but not older adults (p = 0.30). For unrelated intentions, sleep did not improve PM for either age group. While post-encoding N3 was significantly associated with related intentions' execution in young adults (r=0.43, p=0.02), this relationship was not found for older adults (r=-0.07, p=0.763).

The age-related impairment of sleep-dependent memory consolidation extends to PM. Our findings add to an existing body of work suggesting that the link between sleep and memory is functionally weakened in older adulthood.

The age-related impairment of sleep-dependent memory consolidation extends to PM. Our findings add to an existing body of work suggesting that the link between sleep and memory is functionally weakened in older adulthood.Awareness of death has been shown to influence human cognition and behavior. Yet, how mortality threat (MT) impacts our daily social behavior remains elusive. To address this issue, we developed a dyadic experimental model and recruited 86 adults (43 dyads) to complete two computer-based tasks (i.e. competitive and cooperative button-pressing). We manipulated dyads' awareness of death [MT vs neutral control (NC)] and simultaneously measured their neurophysiological activity using electroencephalography during the task. Several fundamental observations were made. First, the MT group showed significantly attenuated competition and slightly promoted cooperation. Second, compared to NC, MT significantly decreased gamma-band inter-brain synchronization (IBS) in the competitive context, which was associated with increased subjective fear of death within dyads. Notably, those effects were context-specific we did not observe comparable results in the cooperative context. Finally, a machine-learning approach was successfully used to discriminate between the MT and NC groups based on accumulated IBS. Together, these findings indicate that MT to some extent mitigates interpersonal competition, and such mitigation might be associated with changes in gamma-band IBS.

Multiple system atrophy (MSA) is associated with disturbances in cardiovascular, sleep and respiratory control. The lateral paragigantocellular nucleus (LPGi) in the ventrolateral medulla (VLM) contains GABAergic neurons that participate in control of rapid eye movement (REM) sleep and cardiovagal responses. We sought to determine whether there was loss of putative GABAergic neurons in the LPGi and adjacent regions in MSA.

Sections of the medulla were processed for GAD65/67 immunoreactivity in eight subjects with clinical and neuropathological diagnosis of MSA and in six control subjects. These putative GABAergic LPGi neurons were mapped based on their relationship to adjacent monoaminergic VLM groups.

There were markedly decreased numbers of GAD-immunoreactive neurons in the LPGi and adjacent VLM regions in MSA.

There is loss of GABAergic neurons in the VLM, including the LPGi in patients with MSA. Whereas these findings provide a possible mechanistic substrate, given the few cases included, further studies are necessary to determine whether they contribute to REM sleep-related cardiovagal and possibly respiratory dysregulation in MSA.

There is loss of GABAergic neurons in the VLM, including the LPGi in patients with MSA. GSK2606414 concentration Whereas these findings provide a possible mechanistic substrate, given the few cases included, further studies are necessary to determine whether they contribute to REM sleep-related cardiovagal and possibly respiratory dysregulation in MSA.Bisphenol F (BPF) is increasingly substituting bisphenol A in manufacturing polycarbonates and consumer products. The cardiometabolic effects of BPF in either humans or model organisms are not clear, and no studies to date have investigated the role of genetic background on susceptibility to BPF-induced cardiometabolic traits. The primary goal of this project was to determine if BPF exposure influences growth and adiposity in male NNIH heterogeneous stock (HS) rats, a genetically heterogeneous population. Littermate pairs of male HS rats were randomly exposed to either vehicle (0.1% ethanol) or 1.125 µg/ml BPF in 0.1% ethanol for 5 weeks in drinking water starting at 3 weeks-of-age. Water consumption and body weight was measured weekly, body composition was determined using nuclear magnetic resonance, urine and feces were collected in metabolic cages, and blood and tissues were collected at the end of the study. BPF-exposed rats showed significantly increased body growth and abdominal adiposity, risk factors for cardiometabolic disease. Urine output was increased in BPF-exposed rats, driving a trend in increased creatinine clearance. We also report the first relationship between a bisphenol metabolizing enzyme and a bisphenol-induced phenotype. Preliminary heritability estimates of significant phenotypes suggest that BPF exposure may alter trait variation. These findings support BPF exposure as a cardiometabolic disease risk factor and indicate that the HS rat will be a useful model for dissecting gene by BPF interactions on metabolic health.Though mule deer (Odocoileus hemionus) persist in robust populations throughout most of their North American distribution, habitat loss, unregulated hunting, and other factors have reduced their historical range in México. Two of the 6 putative subspecies inhabiting México's deserts and Baja California peninsula are of conservation concern, occupying islands in the Pacific Ocean (Odocoileus hemionus cerrosensis on Cedros Island endangered) and Sea of Cortés (Odocoileus hemionus sheldoni on Tiburón Island threatened). Focusing on the desert southwest (n = 448), we sampled Tiburón (n = 22) and Cedros (n = 15) Island mule deer using contemporary samples and natural history museum specimens to complete a phylogeographic evaluation of the species complex, and assess the phylogeography of these insular subspecies. Both insular subspecies formed endemic haplotype lineages, consistent with island biogeographic theory. Bayesian skyline plots were consistent with Holocene demographic expansion. Cedros Island deer were genetically most similar to adjacent mainland Baja California deer, but exhibited a suite of unique haplotypes and reduced genetic variation. link2 Tiburón Island deer haplotypes unexpectedly nested within a mainland lineage found in distant New Mexico, rather than the adjacent mainland Sonoran lineage. Such findings suggest the importance of postglacial climate fluctuations and biotic community turnover in the phylogeographic history of mule deer in the desert southwest. Our genetic data corroborates cultural, archaeological, and phenotypic evidence supporting Cedros and Tiburón deer endemicity and subspecies status. Reduced genetic variation, divergence from mainland populations, and demographic trends on both islands indicate that conservation, monitoring, and management are critical to ensure persistence of these endemic insular subspecies.Little is known about factors that shape sodium consumption behaviors among hospital employees targeted by recent federally funded, sodium-related policy, systems, and environmental change interventions. To address this gap in health promotion practice, we assessed the influence of sodium-related knowledge and attitudes, and health status by self-report on the sodium consumption behaviors of hospital employees targeted by the Los Angeles County Sodium Reduction Initiative. Cross-sectional surveys were administered to 1,213 hospital employees at four Southern California hospitals between 2016 and 2018. Logistic regression models examined the associations between five key sodium consumption behaviors and independent variables of interest (i.e., knowledge and attitudes about sodium, self-reported general health status, and self-reported lifetime hypertension diagnosis), controlling for covariates (i.e., sociodemographic characteristics, body mass index, the type of hospital from which participants were recruited, and the median household income in the hospital's zip code). 1,213 hospital employees completed the survey (completion rate = 50%). The belief that salt intake is harmful was associated with four of five sodium consumption behaviors, while adequate sodium intake knowledge and self-reported "good health" were associated with three of the behaviors. Hypertension diagnosis was associated with only one. Understanding sodium consumption behaviors among healthcare workers can help hospitals improve their employees' health through the promotion of healthier food environments in the workplace and changes in institutional practices.

Detailed CMV kinetics in donor CMV-seropositive, recipient CMV-seronegative (D+R-) transplant recipients receiving preemptive therapy (PET) have not been fully defined.

The study population consisted of PET arm of a randomized CMV prevention trial in D+R- liver transplant recipients. CMV-DNA PCR assays were performed weekly for 100 days using a sensitive assay. Viral load and clinical parameters were compared for patients with or without high-level increase (defined as higher than the group median log10 increase in viral load from baseline after PET initiation).

Among 79 patients, 93.6% (74/79) developed an increase from baseline viral loads of median 120 IU/mL to 3,350 IU/mL; 25.7% (19/74) of the patients had peak levels > 10,000 IU/mL. None of the patients with rise in viral load underwent testing for CMV resistance and viremia resolved with PET with valganciclovir. Patients with high-level increase in viral load had significantly lower rate of recurrent viremia than those without such increase (40%,16/40 vs. 71.8%, 28/39, p=0.004), respectively.

A majority of D+R- recipients had a marked increase in viral load after initiation of PET before resolution of viremia. This phenomenon is associated with lower rates of subsequent recurrent viremia and does not necessarily imply antiviral resistance.

A majority of D+R- recipients had a marked increase in viral load after initiation of PET before resolution of viremia. link3 This phenomenon is associated with lower rates of subsequent recurrent viremia and does not necessarily imply antiviral resistance.Two experiments were conducted to determine the effects of crude protein (CP) level in diets containing coarse wheat bran (CWB) with or without pharmacological levels of Zn (provided by zinc oxide ZnO) on growth performance and fecal DM of nursery pigs. In experiment 1, 360 barrows (Line 200 × 400, DNA, Columbus, NE, initially 5.6 kg) were allotted to 1 of 6 dietary treatments from d 0 to 21 after weaning with 5 pigs per pen and 12 pens per treatment. Treatments included a positive control diet (21% CP) with 3,000 mg/kg Zn in phase 1 and 2,000 mg/kg in phase 2; negative control (21% CP) with 110 mg/kg added Zn, and 4 diets containing 4% CWB and 110 mg/kg added Zn formulated to contain 21%, 19.5%, 18%, or 16.5% CP. The 2 control diets and 21% CP CWB diet contained 1.40% standardized ileal digestible (SID) Lys in phase 1 and 1.35% SID Lys in phase 2, while the 19.5%, 18%, and 16.5% CP diets contained 1.33, 1.25 and 1.20% Lys, respectively, in both phases. Pigs fed the positive control diet containing pharmacological ZnO had increased (P less then 0.