Puckettquinn2175

Using grazing-incidence Rutherford backscattering spectrometry (GI-RBS), the PMMA-NP surface excess (Z*) initially increases rapidly with time and then approaches a constant value at longer times. Consistent with the areal density, Z* is slightly greater at deeper quench depths, which is attributed to the more unfavorable interactions between the PMMA brush and SAN segments. The Z* values at early times are used to determine the PMMA-NP diffusion coefficients, which are significantly larger than theoretical predictions. These studies provide insights into the interplay between wetting and phase separation in PNCs and can be utilized in nanotechnology applications where surface-dependent properties, such as wettability, durability, and friction, are important.The amyloid-β precursor protein (APP) undergoes proteolytic cleavage by α-, β-, and γ-secretases, to determine its fate in Alzheimer's disease (AD) pathogenesis. Recent findings suggest a possible role of O-glycosylation in APP's proteolytic processing. Therefore, we synthesized native and Swedish-double-mutated APP (glyco)peptides with Tyr681-O-GalNAc. We studied conformational changes and proteolytic processing using circular dichroism (CD) spectroscopy and enzyme cleavage assay, respectively. CD analysis was carried out in four solvent systems to evaluate peptide environment and O-glycosylation induced conformational changes. The Swedish mutation and Tyr681-O-GalNAc were the key factors driving conformational changes. Furthermore, the level of α- and β-secretase activity was increased by the presence of mutation and this effect was more pronounced for its glycosylated analogues. Our results suggest that O-glycosylation of Tyr681 can induce a conformational change in APP and affect its proteolytic processing fate toward the amyloidogenic pathway.To match the high capacity of metallic anodes, all-solid-state batteries require high energy density, long-lasting composite cathodes such as Ni-Mn-Co (NMC)-based lithium oxides mixed with a solid-state electrolyte (SSE). However in practice, cathode capacity typically fades due to NMC cracking and increasing NMC/SSE interface debonding because of NMC pulverization, which is only partially mitigated by the application of a high cell pressure during cycling. Using smart processing protocols, we report a single-crystal particulate LiNi0.83Mn0.06Co0.11O2 and Li6PS5Cl SSE composite cathode with outstanding discharge capacity of 210 mA h g-1 at 30 °C. A first cycle coulombic efficiency of >85, and >99% thereafter, was achieved despite a 5.5% volume change during cycling. A near-practical discharge capacity at a high areal capacity of 8.7 mA h cm-2 was obtained using an asymmetric anode/cathode cycling pressure of only 2.5 MPa/0.2 MPa.Metal-organic frameworks (MOFs) provided a versatile platform for the development of new solid protonic electrolytes but faced great challenges regarding their low chemical stability and poor moisture retention capacity. Herein, we presented the proton-conducting study for zirconium-based MOF-802, revealing that MOF-802 possessed excellent features of extra aqueous and acidic stabilities and room-temperature superprotonic conduction with a proton conductivity of 1.05 × 10-2 S cm-1 at 288 K under 98% relative humidity (RH). JHU395 Unfortunately, due to the liberation of water molecules from pores/channels, the proton conductivity of MOF-802 dropped significantly at the temperature above 318 K. To solve this issue, for the first time, MOF-802 was hybridized with poly(vinyl alcohol) (PVA) to form MOF-802@PVA hydrogel composites, where the moisture retention capacity of MOF-802 was greatly improved, giving the high room-temperature proton conductivity over 10-3 S cm-1 under ambient humidity. link2 This work paves a new way to improve the moisture retention capacity and proton-conducting performances of porous proton conductors.Existing local drug delivery systems for periodontitis suffer from poor antibacterial effect and unsatisfied periodontal regeneration. In this study, a smart gingipain-responsive hydrogel (PEGPD@SDF-1) was synthesized as an environmentally sensitive carrier for on-demand drug delivery. The PEGPD@SDF-1 hydrogel was synthesized from polyethylene glycol diacrylate (PEG-DA) based scaffolds, dithiothreitol (DTT), and a novel designed functional peptide module (FPM) via Michael-type addition reaction, and the hydrogel was further loaded with stromal cell derived factor-1 (SDF-1). The FPM exhibiting a structure of anchor peptide-short antimicrobial peptide (SAMP)-anchor peptide could be cleaved by gingipain specifically, and the SAMP was released out of the hydrogel for antibacterial effect in response to gingipain. The hydrogel properties were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), swelling ratio analysis, degradation evaluation, and release curve description of the SAMP and SDF-1. Results in vitro indicated the PEGPD@SDF-1 hydrogel exhibited preferable biocompatibility and could promote the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Antibacterial testing demonstrated that the PEGPD@SDF-1 hydrogel released the SAMP stressfully in response to gingipain stimulation, thereby strongly inhibiting the growth of Porphyromonas gingivalis. Furthermore, the study in vivo indicated that the PEGPD@SDF-1 hydrogel inhibited P. gingivalis reproduction, created a low-inflammatory environment, facilitated the recruitment of CD90+/CD34- stromal cells, and induced osteogenesis. Taken together, these results suggest that the gingipain-responsive PEGPD@SDF-1 hydrogel could facilitate in situ periodontal tissue regeneration and is a promising candidate for the on-demand local drug delivery system for periodontitis.

Patients with chronic underlying diseases are more susceptible to coronavirus disease 2019 (COVID-19) complications. Recent studies showed people living with HIV (PLWH) are not at greater risk than the general population. Few studies have reviewed the impacts of COVID-19 on PLWH. The purpose of this systematic review was to investigate the impact of COVID-19 on patients infected with HIV.

We executed a systematic search using four databases of PubMed, Scopus, Science Direct, and Web of Scienceand screened the records in two steps based on their title/abstract and full text. This study follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist to elevate the validity and reliability of its results.

We reviewed 36 studies. The patients' age was above 20 years in all studies. In almost all studies, the inflammatory parameters were reported high. In most of the studies, all HIV patients completely recovered from the COVID 19 infection. Although CD4 count was not recorded in all studies, the minimum level was reported as 12 cells/µl.

Based on the current review, we concluded that HIV patients at advanced stages (3 or 4) of the disease, whose CD4 counts are low, may show less severe COVID-19 infection symptoms. Similarly, Interference can reduce the severity of immune reactions and subsequent cytokine storms and consequently mitigate the symptoms. Therefore, in most of the studies, the majority of HIV patients showed no severe symptoms and completely recovered from COVID 19 infection.

Based on the current review, we concluded that HIV patients at advanced stages (3 or 4) of the disease, whose CD4 counts are low, may show less severe COVID-19 infection symptoms. Similarly, Interference can reduce the severity of immune reactions and subsequent cytokine storms and consequently mitigate the symptoms. Therefore, in most of the studies, the majority of HIV patients showed no severe symptoms and completely recovered from COVID 19 infection.

MRI is generally considered the modality of choice for the assessment of rotator cuff pathology including tendinosis. Assessment of severity is currently subjective primarily based on tendon morphology and signal intensity. Limited data are available evaluating the inter-observer agreement regarding the assessment of tendinosis severity.

One hundred and twenty-four patients were prospectively enrolled in the study. Patients included were referred to two private radiology practices for shoulder MRI for any reason. Two musculoskeletal radiologists assessed the severity of tendinosis independently. Supraspinatus tendon signal was evaluated with a ROI and compared to the adjacent deltoid muscle signal, generating a ratio of tendon muscle signal, termed the 'objective TM ratio'. The relationship between the subjective assessment of tendinosis severity and objective ratio was examined. Inter-observer agreement was also calculated.

Tendinosis severity was graded as normal in 36 and 11 patients by readers 1 and 2, respectively, mild in 45 and 48 patients, moderate in 26 and 48 patients and severe in 10 and 10 patients. Inter-observer agreement is classified as fair (0.31 kappa co-efficient). Mean objective TM ratio increased on both the PD and TS F2 sequences as the subjective grade of tendinosis severity increased.

A novel means of evaluating rotator cuff tendinosis severity is presented, demonstrated to correlate with the subjective grade of tendinosis severity. Further research is required to assess the utility of such objective measurements across varying imaging protocols whilst understanding the inherent limitations with quantifying signal intensity on MRI.

A novel means of evaluating rotator cuff tendinosis severity is presented, demonstrated to correlate with the subjective grade of tendinosis severity. Further research is required to assess the utility of such objective measurements across varying imaging protocols whilst understanding the inherent limitations with quantifying signal intensity on MRI.

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer with a high incidence of local recurrence and metastasis. Circular RNAs (circRNAs) are implicated in the pathomechanism of TNBC. Here, we investigated the function of circ_0000520 in TNBC and its associated mechanism.

Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot assay were used to measure RNA and protein expression. Cell proliferation was analyzed by cell counting kit-8 (CCK8) assay, flow cytometry and colony formation assay. Cell apoptosis was assessed by flow cytometry. Cell migration ability was analyzed by transwell migration and wound healing assays. Transwell invasion assay was conducted to analyze the invasion ability. link3 Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, and RNA-pulldown assay were performed to verify the interaction between microRNA-1296 (miR-1296) and circ_0000520 or zinc finger protein X-linked (ZFX). Xenograft mice model was established to analyze the role of circ_0000520 in xenograft tumor growth in vivo.

Circ_0000520 expression was upregulated in TNBC tissues and cell lines. Circ_0000520 knockdown suppressed the proliferation, migration, and invasion whereas induced the apoptosis of TNBC cells. miR-1296 was verified as a target of circ_0000520, and circ_0000520 silencing-mediated suppressive effects on the malignant potential of TNBC cells were partly overturned by miR-1296 knockdown. miR-1296 interacted with the 3' untranslated region (3'UTR) of ZFX, and ZFX overexpression partly reversed miR-1296 overexpression-mediated effects in TNBC cells. Circ_0000520 absence reduced ZFX expression by upregulating miR-1296 in TNBC cells. Circ_0000520 silencing suppressed xenograft tumor growth in vivo.

Circ_0000520 contributed to TNBC development by binding to miR-1296 to induce ZFX expression.

Circ_0000520 contributed to TNBC development by binding to miR-1296 to induce ZFX expression.