Postsmith2991

BACKGROUND Cataractogenesis in diabetes mellitus is mainly due to generation of free radicals causing oxidative stress. Antioxidants are known to delay cataractogenesis. Indigenous plants are potential promising sources of antioxidants. OBJECTIVES The present study was done in goat lenses for exploring local antioxidant and anticataract potential of Syzygium cumini (Jamun) and Aegle marmelos (Bael) and comparing their activities. MATERIAL AND METHODS "Lens organ culture technique" was employed using "tissue culture medium 199" (TC 199). Lenses were divided into four groups of 30 each. Group 1 was "Normal Control". In remaining 3 groups, experimental diabetic cataract was produced using dextrose (110 mM). Group 2 "Toxic Control" (untreated experimental diabetic cataract lenses). Group 3 S. cumini seed extract (0.25%) treated lenses. Group 4 A. marmelos leaf extract (0.25%) treated lenses. Biochemical parameters measured in lens homogenates included total soluble lens proteins (index of cataractogenesis), malondialdehyde (index of lipid peroxidation), and superoxide dismutase, glutathione reductase, and glutathione peroxidase (indices of antioxidant enzyme activity). Lens morphology was compared in all groups. RESULTS S. cumini and A. marmelos showed significantly increased activity of all three antioxidant enzymes, preserved total soluble proteins and decreased malondialdehyde (MDA). Lens morphology was well preserved with these extracts. S. cumini aqueous seed extract scored better over A. marmelos. CONCLUSION In goat lenses with dextrose-induced experimental diabetic cataract, S. cumini and A. marmelos showed antioxidant and anticataract properties and preservation of lens morphology (p  less then  0.0001 to 0.05). S. cumini showed better anticataract activity than A. marmelos. BACKGROUND miR-129-5p has been reported to be abnormally expressed and plays an important role in the progression of various malignancies. However, its role in gliomas and its exact molecular mechanism need further research. METHODS AND MATERIALS RT-qPCR was performed to evaluate miR-129-5p and HOXC10 mRNA expression levels in tissues and cell lines. Cell proliferation was detected via Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) and clone formation assays. Luciferase assays were used to validate the binding of seeds between miR-129-5p and HOXC10. A tumor xenograft model was developed to study the effect of miR-129-5p on glioma growth in vivo. RESULTS miR-129-5p was expressed at low levels in glioma tissues and cell lines. miR-129-5p overexpression inhibited glioma proliferation, migration and invasion. miR-129-5p negatively and directly targeted HOXC10. At the same time, HOXC10 was upregulated in glioma cancer, and HOXC10 knockdown inhibited cell proliferation, migration and invasion. CONCLUSION miR-129-5p inhibits glioma development by altering HOXC10 expression and may therefore serve as a new diagnostic marker and therapeutic target for glioma in the future. OBJECTIVE The associations between viruses and the cancer have been conducted in several studies while there has been no systematic review and meta-analysis about the association between viral infections and thyroid cancer (TC). Therefore, we investigated the association between viral infection and TC risk. METHODS Systematic search was done from 1994 to 2019 in Web of sciences (ISI), PubMed, and Scopus databases. Pooled logarithm of odds ratio (OR) and their corresponding 95 % confidence interval (CI) and pooled prevalence of viral infections were calculated to find the association between the viral infections and TC risk and overall prevalence of the viral infections in TC. RESULTS Twenty-three of 852 original articles were selected and included in the study. According to the results of the random effect meta-analysis, the pooled prevalence of viral infections in the TC patients was 37 % (95 % C. I = 22 %-55 %). In addition, there was a significant association between viral infections (log (OR) = 1.51, 95 % credible interval = 0.68-2.39) and TC risk. The highest associations were observed between TC risk and Simian Vacuolating Virus 40 (SV40) and B19 infections, respectively. The lowest non-significant association was found between TC risk and Poliovirus type 1 infection. The significantly heterogeneity was observed between included studies (Q test p-value less then 0.001; I2 = 73.82 %; τ2 = 1.08, 95 % Cr. I = 0.47-1.94). CONCLUSIONS Results clearly demonstrated the potential pathogenetic association between viral infections and increased risk of TC. Edmund Randerath must be counted among the pioneers of nephropathology. However, his early experimental proof of glomerular proteinuria is hardly known, even among experts. The same applies to his political role in the Third Reich and his denazification in the post-war period. Against this background, this study deals with the life and career of Randerath. It focuses on (1) Randerath's political stance and professional progress between 1933 and 1945, (2) his life and his position in German pathology after 1945, and (3) the (inter)national reception of Randerath's work. The paper is based on mostly unevaluated sources of various archives and on the analysis of the relevant research literature. It demonstrates that Edmund Randerath willingly served the Nazi regime. In return, he succeeded in expanding his career in the Third Reich. However, after 1945 he moved up to even higher positions He was successively appointed full professor (1947), dean (1950) and rector (1956) of Heidelberg University. Moreover, he played a decisive role in the reconstitution of the Deutsche Gesellschaft für Pathologie (German Society for Pathology) (1948) and was later elected president (1960). But despite his pioneering achievements in (nephro)pathology, he found only limited access to the international scientific community. BACKGROUND Long noncoding RNAs (lncRNAs) play crucial role in formation and progression of tumors. DNA methylation has become increasingly recognized as a frequent event of epigenetic alterations and one of the primary mechanisms of gene inactivation. The research aims to investigate the biofunction of a novel lncRNA in LSCC. METHODS qRT-PCR, BGS, and MSP methods were employed to measure the relative expression level and methylation status of LINC00886. Additionally, we examined the effects of LINC00886 on cells proliferation and invasion using LINC00886 over-expression. Nude mouse xenograft models were conducted to assess LINC00886 effects on LSCC growth in vivo. MLT-748 solubility dmso High-throughput sequencing technology and Western blot assay were carried out to have an in-depth study of the downstream target genes and signaling pathways in which LINC00886 may participate. RESULTS The remarkable downregulation of LINC00886 was observed in tumor tissues and laryngeal cancer cell lines. The significant decrease of LINC00886 was correlated with pathological grade in LSCC tissues.