Beyerrusso0658

This study analysed the mycobiota on exoskeleton debris of the crab Neohelice granulata collected from an alkaline salt marsh and assessed the in vitro enzyme ability of selected isolates at different temperatures and pH. Exoskeleton fragments were incubated in moist chambers on paper and on agar medium. Growth and enzyme ability of selected fungi were also evaluated in agar media with 0.5 % casein, 1% Tween®20, and Chitin-Azure® by the production of a halo/growth ratio. We identified 22 fungal species using both methods. Since the two isolation methods added information to one another, both ones are necessary to recover the cultivable mycobiota associated with the exoskeleton debris. All fungi showed greater levels of enzyme activity in alkaline than acid medium with Tween®20. The halo diameter on casein and chitin varied according to the fungal isolate and pH. Most fungi had a larger halo at 4°C than at the other temperatures tested. Clonostachys rosea showed the greatest activity in all media at 4ºC. We conclude that exoskeletons of the N. granulata are a source of fungi able to produce enzyme activities that show differences upon incubation conditions to which they are cultivated such as ones including specific temperatures and pH values.The lowlands of mid-latitude South America comprise complex temperate ecoregions characterized by a unique biodiversity. However, the processes responsible for shaping its species diversity are still largely unknown. Turnera sidoides subsp. carnea is a variable subspecies occurring in the lowlands of northeastern Argentina and Uruguay, extending to southern Paraguay and Brazil. It constitutes a good model to perform evolutionary studies. Here we used an integrative approach to understand the process of diversification within this subspecies and to increase the knowledge concerning patterns and processes responsible for shaping the species diversity in the temperate lowlands of South America. The results provided strong evidences that this subspecies is an autopolyploid complex per se, being in an active process of intrasubspecific diversification. Morphological and genetic data show that the diversity of T. sidoides subsp. carnea is in congruence with the great past and present abiotic and biotic variability of the mid-latitude South American lowlands. The evolutionary history of this subspecies is consistent with past fragmentation and allopatric differentiation at diploid level. Geographic isolation and local adaptation would have promoted strong morphological, ecological, and genetic differentiation, resulting in two morphotypes and different genetic groups indicative of incipient speciation.In this study, it was determinated the essential oil of cultivated apple mint, Mentha suaveolens Ehrh. composition and in vitro antibacterial activity of against 11 fish pathogen bacteria including Gram-positive (Staphylococcus warneri, Staphylococcus sp., Lactococcus garvieae, Vagococus salmoninarum) and Gram-negative (Aeromonas hydrophila, Aeromonas sobria Aeromonas cavieae, Vibrio anguillarum, Pseudomonas aeroginosa, Yersinia ruckeri, Edwardsiella tarda) by using agar diffusion assay. The main component of M. suaveolence oil was obtained as piperitenone oxide. The essential oil exhibited strong inhibitory activity such as inhibition zone sizes 30-50mm at 250-1000 µL mL-1 concentrations against V. anguillarum; 16-20mm at 31.25-125 µL mL-1 concentrations against P. aeroginosa; 15-18mm at 500-1000 µL mL-1 concentrations against A. sobria. However, it was found to be moderately effective against E. tarda (8-15 mm), Y. ruckeri (9-12mm), S. warneri (9-10mm), V. salmoninarum (9mm) and Staphylococcus sp. (8-9mm). The essential oil showed weak inhibitory activity against A. cavieae (5-8), A. hydrophila (6-7mm), L. garvieae (5-7mm). Thus, effect of essential oil of M. suaveolens on immune response and disease resistance against Vibrio anguillarum, A. sobria and P. aeroginosa should be investigated in vivo in cultured fish species in future studies.% We show that codimension one distributions with at most isolated singularities on certain smooth projective threefolds with Picard rank one have stable tangent sheaves. The ideas in the proof of this fact are then applied to the characterization of certain irreducible components of the moduli space of stable rank 2 reflexive sheaves on $\p3$, and to the construction of stable rank 2 reflexive sheaves with prescribed Chern classes on general threefolds. We also prove that if $\sG$ is a subfoliation of a codimension one distribution $\sF$ with isolated singularities, then $\sing(\sG)$ is a curve. As a consequence, we give a criterion to decide whether $\sG$ is globally given as the intersection of $\sF$ with another codimension one distribution. Turning our attention to codimension one distributions with non isolated singularities, we determine the number of connected components of the pure 1-dimensional component of the singular scheme.Aetosauria represents a remarkable clade of armored pseudosuchians in which some of its oldest members are recovered from late Carnian units of Brazil. Three species are known the mid-sized aetosaur Aetosauroides scagliai, which also occurs in Argentina, and two small-sized species, Aetobarbakinoides brasiliensis and Polesinesuchus aurelioi. We provide a detailed description and comparative analysis of the axial skeleton of Aetosauroides, identifying some diagnostic features as variable. These include the deep pocket pit lateral to the base of the neural spine, the presence of the infradiapophyseal laminae and the lateral fossa ventral to the neurocentral suture. These features are not found in smaller and immature Aetosauroides specimens, resembling the condition found in Polesinesuchus, which is based solely on a juvenile individual, as revealed by osteoderm microstructure analysis. As Polesinesuchus cannot be anatomically differentiated from other small individuals of Aetosauroides, we propose it as a junior synonym of Aetosauroides scagliai. Our results shrink the number of putative 'dwarf' aetosaurs, indicating that morphological variation related to ontogeny affects aetosaur taxonomy and phylogeny.A 62-year-old woman presented to our clinic in April 2021 with a painful right eye and increased light sensitivity for 1 week, 30 years after laser in situ keratomileusis (LASIK) in both eyes for myopia of -2.5 diopters. There were no complaints of the left eye. There were no abnormalities in her medical history. The uncorrected distance visual acuity (UDVA) of the right eye at presentation was 20/20. Slitlamp biomicroscopy revealed epithelial ingrowth on the right eye with stromal edema and inflammation and fine keratic precipitates on the endothelium (Figures 1 and 2JOURNAL/jcrs/04.03/02158034-202109000-00024/figure1/v/2021-08-31T192739Z/r/image-tiffJOURNAL/jcrs/04.03/02158034-202109000-00024/figure2/v/2021-08-31T192739Z/r/image-tiff). The cornea of the left eye was unremarkable. The anterior chamber of the right eye showed 1 + cells (SUN grading). There were no abnormalities in the posterior segment. Figure 3JOURNAL/jcrs/04.03/02158034-202109000-00024/figure3/v/2021-08-31T192739Z/r/image-tiff shows the corneal epithelial ingrowth with a swept-source optical coherence tomography (OCT) and Figure 4JOURNAL/jcrs/04.03/02158034-202109000-00024/figure4/v/2021-08-31T192739Z/r/image-tiff an axial corneal Scheimpflug tomography map. What is your treatment advice for this patient from a short- and long-term perspective?A modification of intraocular lens (IOL) implantation, using the 4-flanged IOL fixation technique, through a 2.4 mm corneal incision using a standard cartridge and injector, is presented. An IOL with 4 eyelets is used, through which a 6-0 polypropylene suture is threaded on one side and then loaded into a provided cartridge and inserted to the anterior chamber through a 2.4 mm corneal incision. see more Using the handshake technique, the suture ends are eventually threaded through the eyelets and secured outside the eye with the creation of 4 flanges. This technique was used in 6 eyes of 5 consecutive patients with the absence of capsular support. During all follow-up visits, the IOLs were well centered and stable, and the flanges were buried in the sclera and covered with the conjunctiva. No complications were recorded. This modification simplifies the technique and reduces the well-known complications of large corneal incisions.The endothelial cell is a critical structure within the cornea and is responsible for maintaining corneal clarity through its pump function. Endothelial cells are lost over time naturally but can be injured medically, surgically, or as a part of various dystrophies. Monitoring of endothelial cells can be performed clinically or more formally with specular microscopy. In cases of significant compromise, endothelial cells can be transplanted by various endothelial keratoplasty techniques. The future pipeline is bright for possible endothelial cell regeneration and rehabilitation. This article reviews these topics in depth to provide a comprehensive look at the structure and function of the endothelial cell, etiologies of endothelial cell damage, detailed review of iatrogenic causes of endothelial cell loss, and management strategies.

To compare myopia and astigmatic correction after small-incision lenticule extraction (SMILE) with or without prophylactic crosslinking (SMILE Xtra).

Shenyang Aier Eye Hospital, Central South University, China.

Retrospective study.

Patients with comparable manifest sphere and cylinder undergoing SMILE Xtra or SMILE were enrolled. The crosslinking (CXL) energy was 2.7 J/cm2. Only right eyes were selected. Visual and refractive changes were evaluated for 1 year. Astigmatic correction was analyzed using Alpins method.

Thirty-six eyes undergoing SMILE Xtra and 40 eyes undergoing SMILE were enrolled. The uncorrected distance visual acuity at 1-day visit was lower after SMILE Xtra than that after SMILE (P = .01). At 12 months, the mean manifest refraction spherical equivalent (MRSE) and manifest cylinder were 0.08 ± 0.32 diopters (D) and -0.29 ± 0.23 D in SMILE Xtra group, whereas -0.25 ± 0.29 D and -0.22 ± 0.19 D in SMILE group (P < .01 and P = .135), respectively. Thirty-four eyes (94%) and 32 eyes (89%) in SMILE Xtra group and 36 eyes (91%) and 39 eyes (98%) in SMILE group exhibited target MRSE and manifest cylinder within ±0.50 D (P = .771 and P = .294), respectively. Compared with SMILE group, spherical correction index (SCI), correction index (CI), and difference vector were higher in SMILE Xtra group since 1-week follow-up (all P < .05). SCI and CI were slightly more than 1.0 after SMILE Xtra even at postoperative 12-month follow-up.

With CXL protocol of 30 mW/cm2 for 90 seconds, SMILE Xtra exhibited comparable astigmatic correction with SMILE up to 1-year follow-up, although slight spherical equivalent and astigmatic overcorrection were evident after SMILE Xtra.

With CXL protocol of 30 mW/cm2 for 90 seconds, SMILE Xtra exhibited comparable astigmatic correction with SMILE up to 1-year follow-up, although slight spherical equivalent and astigmatic overcorrection were evident after SMILE Xtra.