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001), with an increasing difference as work rate increased (P < .001). Neither cycle rate nor work per cycle differed between inclines when compared at identical submaximal work rate (P > .16).
The longer poling times utilized in uphill XC sit-skiing enable more work per cycle and better gross efficiency, thereby allowing skiers to achieve a higher WRpeak compared to flat XC sit-skiing. However, the similar values of peak oxygen uptake between inclines indicate that XC sit-skiers can tax their cardiorespiratory capacity similarly in both conditions.
The longer poling times utilized in uphill XC sit-skiing enable more work per cycle and better gross efficiency, thereby allowing skiers to achieve a higher WRpeak compared to flat XC sit-skiing. However, the similar values of peak oxygen uptake between inclines indicate that XC sit-skiers can tax their cardiorespiratory capacity similarly in both conditions.
Rating of perceived exertion (RPE) is a widely used tool to assess subjective perception of effort during exercise. The authors investigated between-subject variation and effect of exercise mode and sex on Borg RPE (6-20) in relation to heart rate (HR), oxygen uptake (VO2), and capillary blood lactate concentrations.
A total of 160 elite endurance athletes performed a submaximal and maximal test protocol either during cycling (n = 84, 37 women) or running (n = 76, 32 women). The submaximal test consisted of 4 to 7 progressive 5-minute steps within ∼50% to 85% of maximal VO2. For each step, steady-state HR, VO2, and capillary blood lactate concentrations were assessed and RPE reported. An incremental protocol to exhaustion was used to determine maximal VO2 and peak HR to provide relative (%) HR and VO2 values at submaximal work rates.
A strong relationship was found between RPE and %HR, %VO2, and capillary blood lactate concentrations (r = .80-.82, all Ps < .05). The between-subject coefficient of variation (SD/mean) for %HR and %VO2 decreased linearly with increased RPE, from ∼10% to 15% at RPE 8 to ∼5% at RPE 17. Compared with cycling, running induced a systematically higher %HR and %VO2 (∼2% and 5%, respectively, P < .05) with these differences being greater at lower intensities (RPE < 13). At the same RPE, women showed a trivial, but significantly higher %HR and %VO2 than men (<1%, P < .05).
Among elite endurance athletes, exercise mode influenced RPE at a given %HR and %VO2, with greater differences at lower exercise intensities. Athletes should manage different tools to evaluate training based on intensity and duration of workouts.
Among elite endurance athletes, exercise mode influenced RPE at a given %HR and %VO2, with greater differences at lower exercise intensities. Athletes should manage different tools to evaluate training based on intensity and duration of workouts.The aim of this study was to examine the construct and known-groups validity of the total score of five items adapted from the Community Healthy Activities Model Program for Seniors (CHAMPS) questionnaire to measure outdoor walking (CHAMPS-OUTDOORS) in older adults. Tanzisertib Data from the baseline assessment of the Getting Older Adult OUTdoors (GO-OUT) trial were used. Construct validity of the CHAMPS-OUTDOORS used objective measures of outdoor walking (accelerometry-GPS), Ambulatory Self-Confidence Questionnaire, RAND-36, 6-min walk test, 10-m walk test, and Mini-Balance Evaluation System Test. For known-groups validity, we compared the CHAMPS-OUTDOORS of those who walked less then or ≥1.2 m/s. Sixty-five participants had an average age of 76.5 ± 7.8 years. The CHAMPS-OUTDOORS was moderately correlated with total outdoor walking time (r = .33) and outdoor steps (r = .33) per week measured by accelerometry-GPS, and weakly correlated with Mini-Balance Evaluation System Test score (r = .27). The CHAMPS-OUTDOORS did not distinguish known groups based on crosswalk speed (p = .33). The CHAMPS-OUTDOORS may be used to assess outdoor walking in the absence of accelerometry GPS. Further research examining reliability is needed.
Movement behaviors (physical activity, sedentary time, and sleep) established in early childhood track into adulthood and interact to influence health outcomes. This study examined the associations between neighborhood characteristics and weather with movement behaviors in preschoolers.
A subset of Canadian Healthy Infant Longitudinal Development birth cohort (n = 385, 50.6% boys) with valid movement behaviors data were enrolled at age 3years and followed through to age 5years. Objective measures of neighborhood characteristics were derived by ArcGIS software, and weather variables were derived from the Government of Canada weather website. Random forest and linear mixed models were used to examine predictors of movement behaviors. Cross-sectional analyses were stratified by age and season (winter and nonwinter).
Neighborhood safety, temperature, green space, and roads were important neighborhood characteristics for movement behaviors in 3- and 5-year-olds. An increase in temperature was associated with greater light physical activity longitudinally from age 3 to 5years and also in the winter at age 5years in stratified analysis. A higher percentage of expressways was associated with less nonwinter moderate to vigorous physical activity at age 3years.
Future initiatives to promote healthy movement behaviors in the early years should consider age differences, neighborhood characteristics, and season.
Future initiatives to promote healthy movement behaviors in the early years should consider age differences, neighborhood characteristics, and season.A rapid, sensitive, and reliable ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed to simultaneously determine the major bioactive components of Xiaoyan Lidan Formula (XYLDF) in rat plasma, using sulfamethoxazole as the internal standard (IS). The seven major bioactive components are andrographolide, dehydroandrographolide, enmein, 1-methoxicabony-β-carboline, 4,5-dimethoxy-canthin-6-one, 4-methoxy-5-hydroxy-canthin-6-one, and 1-hydroxymethyl-β-carboline. After pretreating by protein precipitation with methanol, separation was performed on a UPLC C18 column using gradient elution with a mobile phase consisting of acetonitrile and 0.1% formic acid at a flowing rate of 0.7 mL/min. Detection was performed on TSQ Quantum mass spectrometry set at the positive/negative ionization and multiple reaction monitoring (MRM) mode. The intra- and inter-day precision were less than 9.8%, whereas the intra- and inter-day accuracy were within ± 13.4%. The method was validated and applied to compare the pharmacokinetic profiles of the analytes in serum of Alpha-naphthylisothiocyanate (ANIT)-induced cholestasis and control rats after oral administration of XYLDF. The results showed remarkable differences in pharmacokinetic properties of the analytes between cholestatic (model) and control groups, thereby providing essential scientific information for better understanding of mechanism of XYLDF and a reference for its clinical applications.Ionizing radiation leads to release of free radicals into the systemic circulation from irradiated tissues. These free radicals cause oxidative stress in distant organs. Oxidative status may be reversed by naturally occurring antioxidant agents. The aim of this study was to investigate protective and antioxidant effects of Nigella sativa oil (NSO) and thymoquinone (TQ) in kidney tissue of rats exposed to cranial irradiation. Forty-eight Sprague-Dawley rats were divided into six groups IR group received irradiation (IR) to total cranium plus saline; IR plus NSO group received IR and NSO; IR plus TQ group received IR and TQ; sham group did not receive NSO, TQ or IR; control group of TQ received dimethyl sulfoxide; control group of NSO received saline. Total oxidant status (TOS), oxidative stress index (OSI) and lipid hydroperoxide (LOOH) levels were studied as oxidative parameters, and total antioxidant status (TAS), total sulfhydryl levels, paraoxonase (PON), ceruloplasmin (Cp) and arylesterase activities were determined as antioxidative parameters in the kidney tissue of rats. Kidney TOS, OSI and LOOH levels were significantly lower in IR plus TQ, IR plus NSO and sham groups compared to IR group (p less then 0.001). TAS, PON and Cp activities in IR group were significantly lower compared to the control group (p less then 0.001). PON and Cp activities were significantly higher in IR plus NSO and IR plus TQ groups compared to IR group (p less then 0.001). In conclusion, free radicals generated by cranial ionizing radiation exposure cause oxidative stress in kidney. NSO and TQ exhibit protective and antioxidant effects against oxidative damage in rats.Erythropoietin (Epo) is a pleiotropic cytokine, essential for erythropoiesis. Epo and its receptor (Epo-R) are produced by several tissues and it is now admitted that Epo displays other physiological functions than red blood cell synthesis. Indeed, Epo provides cytoprotective effects, which consist in prevention or fight against pathological processes. This perspective article reviews the various protective effects of Epo in several organs and tries to give a proof of concept about its effects in the lung. The tissue-protective effects of Epo could be a promising approach to limit the symptoms of acute and chronic lung diseases.G-quadruplexes (G4) are secondary four-stranded DNA helical structures consisting of guanine-rich nucleic acids, which can be formed in the promoter regions of several genes under proper conditions. Several cancer cells have been shown to emerge from genomic changes in the expression of crucial growth-regulating genes that allow cells to develop and begin to propagate in an undifferentiated state. Recent attempts have focused on producing treatments targeted at particular protein products of genes that are abnormally expressed. Many of the proteins found are hard to target and considered undruggable due to structural challenges, protein overexpression, or mutations that affect treatment resistance. The utilization of small molecules that stabilize secondary DNA structures existing in several possible oncogenes' promoters and modulate their transcription is a new strategy that avoids some of these problems. In this review, we outline the function of G-quadruplex stabilization in cancer by small-molecules with the aim to improve cancer therapy.The residue of doxycycline in food can cause harm to human. Therefore, the detection of doxycycline residue is necessary. Herein, a ratiometric fluorescent probe was designed based on sulfur quantum dots (S dots) and Ca2+. Due to static quenching and inter filter effect between doxycycline and S dots, doxycycline quenched fluorescence of S dots at 450 nm. Meanwhile, doxycycline and Ca2+ formed fluorescent complex through coordination to produce new peak at 520 nm. The ratio of fluorescence intensity (F520/F450) and doxycycline concentration showed good linear relationship with detection limit of 0.19 μM. The fluorescence color of S dots/Ca2+ changed from blue to light green with increasing doxycycline concentration, which was applied for visual semi-quantitative detection of doxycycline. Moreover, the method was used for detecting doxycycline in milk and fish samples with recoveries in the range of 91%-110%. The method showed good application potential in detection of doxycycline in food samples.
