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Shale matrix permeability is one of the most important parameters for characterizing a source rock reservoir and for predicting hydrocarbon production. The low permeability value and the presence of induced fractures during core retrieval and transportation make the accurate measurement of the true permeability values for source rocks a significant challenge for the industry. The steady state flow method and the transient pressure pulse decay method on core plug samples mainly measure the permeability of fractures when fractures are present. While the Gas Research Institute (GRI) method that uses pressure decay on crushed rock samples was designed to overcome this difficulty associated with the induced fractures, its measurement results are reported to be sensitive to the particle size of crushed rock samples and also need correction of Knudsen diffusion effect. Moreover, the GRI method is limited to the unconfined stress condition. This work develops a practical method to measure the matrix permeability values from fractured source rock samples by extending the commonly used pressure pulse decay method. A source rock sample with fractures can be more accurately described by a dual-continuum system consisting of a fracture continuum and a matrix continuum. During the pulse decay test, the initial flow across the source rock sample is dominated by the fracture continuum because it has much higher permeability values than those for the rock matrix. Thus, the initial gas pressure signals from the test are used to estimate the fracture permeability. During the late-stage of a pulse decay test, the flow process within the rock sample is controlled by the rock matrix. The observed pressure signals at this stage are used for estimating matrix permeability. The method is based on the analytical solution to gas flow in the fractured rock sample and relatively simple to apply in practice. Both fracture and matrix permeability's dependence on the effective stress can be assessed with this method.European freshwater pearl mussel (Margaritifera margaritifera) populations are declining despite a growing effort to conserve them. Here we used a combination of local ecological knowledge (LEK) and field sampling to assess past and current distribution and conservation status of this endangered species in Cávado and Neiva Rivers (Portugal). We performed face-to-face interviews in both rivers and sampled the entire area where the respondents confirmed the historical presence of this species. Abiotic characterization, water quality and fish diversity were also assessed in both rivers. We found that freshwater pearl mussels are now possibly extinct in Cávado River but almost 50% of the respondents confirm its presence in the past, especially elders that lived in villages near its historical distribution. To the contrary, and although the species is still present in Neiva River, only 3.8% of the respondents remembered its presence in the past. In both rivers, respondents suggested pollution as the most important explanation for the freshwater pearl mussels decline. However, nowadays both rivers present excellent water quality and trout Salmo trutta (the freshwater pearl mussel fish host) is still abundant. Since we identified the areas where the species was present in a recent past, this information is vital for possible management actions with the aim of re-introduce or increase the abundance of M. margaritifera populations and/or for the rehabilitation of habitats in both rivers. We also highlight the vital importance of getting LEK, mainly from elders, in order to avoid shifting baseline syndromes and to get qualitative accurate information of past references and/or experience with historical conditions. Results reported here reinforce concern about the conservation status of freshwater pearl mussel populations in Portugal and can be used to guide future research and management initiatives to better conserve this species.Measures to improve the use of phosphorus (P), either by improved efficiency or reuse, are needed worldwide in order to preserve a finite resource and ensure that farmers have access to it. Currently, the rapidly growing global population has generated an increased demand for this mineral. Sustainably disposing for the massive amount of globally produced biosolids and alternative sources of P for agriculture are two major challenges to address. In this scenario, biosolids-derived biochar (BBC) has been presented as a win-win opportunity. However, the BBC-P dynamics in soil over consecutive cropping seasons remain unclear. Direct (first and second cropping season) and residual (third and fourth cropping season) effects of BBC on soil P fractions, P uptake and corn grain yield were assessed. Additionally, the relationships between soil P pools and grain yield were investigated by multivariate and multiple linear regression analysis. In a field experiment, BBC produced at 300 °C (BC 300) and 500 °C (BC 500) were applied to an Oxisol at a rate of 15 t ha-1. Soil total P and its fractions (organic P, inorganic P, and available P) were determined. Phosphorus uptake and corn grain yield were also evaluated. BBC, regardless of pyrolysis temperature, increased soil total P and all P fractions. Moreover, BBC maintained high soil P contents for at least two years after stopping its application. These results suggest that BBC may act as a slow-release P fertilizer. Surprisingly, soil P fractions were unaffected by different pyrolysis temperatures, but BC 300 promoted higher grain yield than BC 500 in the third and fourth cropping seasons. Overall, the results confirmed that under direct application both biochars can replace mineral fertilization for corn production; and when considering the residual effect, BC 300 showed a higher potential to be utilized as a soil amendment for P supply.Wuhan was the first city to adopt the lockdown measures to prevent COVID-19 spreading, which improved the air quality accordingly. This study investigated the variations in chemical compositions, source contributions, and regional transport of fine particles (PM2.5) during January 23-February 22 of 2020, compared with the same period in 2019. The average mass concentration of PM2.5 decreased from 72.9 μg m-3 (2019) to 45.9 μg m-3 (2020), by 27.0 μg m-3. It was predominantly contributed by the emission reduction (92.0%), retrieved from a random forest tree approach. The main chemical species of PM2.5 all decreased with the reductions ranging from 0.85 μg m-3 (chloride) to 9.86 μg m-3 (nitrate) (p less then 0.01). Positive matrix factorization model indicated that the mass contributions of seven PM2.5 sources all decreased. However, their contribution percentages varied from -11.0% (industrial processes) to 8.70% (secondary inorganic aerosol). Source contributions of PM2.5 transported from potential geographical regions showed reductions with mean values ranging from 0.22 to 4.36 μg m-3. However, increased contributions of firework burning, secondary inorganic aerosol, road dust, and vehicle emissions from transboundary transport were observed. This study highlighted the complex and nonlinear response of chemical compositions and sources of PM2.5 to air pollution control measures, suggesting the importance of regional-joint control.The Kinzl Glacier is a unique dendritic-type glacier of the Peruvian Cordillera Blanca and is surrounded by well-developed Little Ice Age (LIA) moraines. Based on field observations and analysis of historical photographs (since 1932) and remotely sensed images (since 1948), we interpret distinct mechanisms of landslides in these moraines and link them to glacier retreat and thinning. Three types of landslides are distinguished according to the cross-profile morphology (i) type "N", (ii) type "M" and (iii) type "A". Our data show that sliding of type "N" is an ice-contact slope failure that occurs as a gradual process simultaneously to glacier downwasting. Epigenetics inhibitor In contrast, type "A" can occur at any time once the glacier has downwasted below the sliding plane and cannot buttress the nearly vertical inner slopes of the moraine anymore. We further argue that the type "M" can gradually evolve from type "N" or can occur as an episodic event. Probably due to overconsolidation of moraine material, landslides of types "N" and "M" keep their shape during sliding and move in form of several hundred meters long unbroken blocks. In contrast type "A" is internally disintegrated during landsilding. All investigated landslide types are characterized by increased width-length ratio and movement perpendicular to the direction of the flow of the glacier. We opine that the occurrence of these landslide types is directly or indirectly associated with glacial ice loss occurring since the end of the LIA. The observed landslides in the LIA moraines of the Kinzl Glacier are unique in the regional context considering their estimated size on the order of 106 m3 and contribute significantly to the paraglacial adjustment of moraine slopes and landform evolution in the post-LIA context. Apart from their role in moraine evolution, these landslides can trigger hazardous cascading process-chains in high-alpine environments.Tree cores and bark were sampled from jack pine trees at 18 sites in the Athabasca Oil Sands Region (AOSR) of Alberta, Canada, to investigate spatial and temporal trends of polycyclic aromatic compounds (PACs). Spatial trends were investigated in the bark samples, where ΣPAC concentrations ranged from 75 to 3615 ng/g. Highest concentrations were observed from trees within 40 km of the nearest mining or upgrading facility perimeter fence, in line with previous deposition studies in the AOSR. The sampled tree cores were separated into segments representing 5 years of growth/atmospheric collection by counting tree rings. A significant increase in PAC concentrations over the lifetime of the tree was observed at sites with the highest PAC concentrations, and the average % increase in concentration from 1970 to 2015 was in line with average % growth in bitumen extraction in the AOSR. Finally, the concentrations in the tree core segments representing collection from 2010 to 2015 were converted into an atmospheric PAC concentration using previously published wood-air partition coefficients. The calculated atmospheric concentrations were within the same range as concentrations reported from the passive atmospheric sampling network in this region. The importance of site location is highlighted, with forest edge sites providing an improved comparison for atmospheric exposure and deposition. This is the first study to use tree cores to calculate an atmospheric concentration of PACs, demonstrating the applicability of this methodology for providing historic atmospheric data.Deciphering the succession dynamics of dominant and rare taxa is crucial to understand the stability and ecosystem functions of biofilm communities. However, the essential laws of the succession dynamics based on dominant and rare taxa were still unenlightened. Herein, we investigated the succession dynamics of dominant and rare genera in multi-species biofilms developed in flow cells fed with 10 and 40 mg-TOC/L LB broth. The relative abundance of dominant genera (Enterobacteria and Acinetobacter) decreased remarkably (from 94.63% to 73.22%) in 10 mg-TOC/L LB broth, whereas they kept relatively steady (93.75 ± 4.23%) along with the cultivation time in 40 mg-TOC/L LB broth. Fluorescence in situ hybridization showed that rare genera tended to form clusters at both concentrations, while weaker dispersal of dominant genera caused patchier biofilm structures in 10 mg-TOC/L LB broth compared to that in 40 mg-TOC/L LB broth. Null model analyses further demonstrated that the stochastic ecological drift was more pronounced in the community assembly of biofilms in 10 mg-TOC/L LB broth (73.