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Cruciferous and Allium vegetables are rich in organic sulphides that can act on the metabolism of carcinogens and therefore used as anti-cancer and suppressing agents while dietary fibres and plant sterols may improve intestinal health and prevent intestinal diseases. Thus, we recommend a diet rich in fruits, vegetables, and grains as its content of phytochemicals may have the potential to prevent or improve a broad sweep of various diseases.The aim of this work was to study the characteristics, kinetics and electronic distribution of photo-fermentation hydrogen production (PFHP) with Zn2+ addition then gave the main results that the addition of Zn2+ can effectively improve hydrogen production with an increasing of 1-5 mg/L Zn2+ concentration. The maximum hydrogen yield of 592 ± 13 mL and shortest lag time of 4.67 h were obtained at 2 mg/L Zn2+. 26.42% of the substrate energy was diverted to H2. Modified Gompertz and Hane-Levenspiel models were applied to evaluate the effect of Zn2+ on PFHP by mixed bacteria HAU-M1, the constants n and m obtained by fitting models were 14.97 and 58.79, respectively, indicating the fermentation system was noncompetitive inhibition, the predicted critical Zn2+ concentration was 40.83 mg/L.In the present study, an exopolysaccharide (EPS)-producing bacterial strain was isolated from the Eastern Sea (Sokcho Beach) of South Korea and identified as Sphingobium yanoikuyae BBL01. Media optimization was performed using response surface design, and a yield of 2.63 ± 0.02 g/L EPS was achieved. Purified EPS produced using lactose as the main carbon source was analyzed by GC-MS and found to be composed of α-D-xylopyranose (28.6 ± 2.0%), β-D-glucopyranose (21.0 ± 1.6%), α-D-mannopyranose (18.5 ± 1.2%), β-d-mannopyranose (13.1 ± 1.4%), β-D-xylopyranose (10.2 ± 2.1%), α-d-talopyranose (5.9 ± 1.1%), and β-d-galacturonic acid (2.43 ± 0.8%). Interestingly, different carbon sources (glucose, galactose, glycerol, lactose, sucrose, and xylose) showed no effect on EPS monomer composition, with a slight change in the mass percentage of various monosaccharides. Purified EPS was stable up to 233 °C, indicating its possible suitability as a thickening and gelling agent for food-related applications. EPS also showed considerable emulsifying, flocculating, free-radical scavenging, and metal-complexion activity, suggesting various biotechnological applications.In this study, polyvinyl alcohol (PVA) gel beads were used to aid the partial nitrification and anammox process (PN/A) for treating low-strength ammonia wastewater. When treating synthetic and municipal wastewater, the reactor amended with PVA gel beads achieved a total nitrogen removal efficiency of 75.1 ± 8.4% and 66.6 ± 7.0% respectively, while the control reactor without PVA gel beads achieved 63.2 ± 7.8% and 28.2 ± 11.5% respectively. Dissolved oxygen ( less then 0.5 mg O2 L-1) and substrate diffusion in porous PVA gel beads facilitated the formation of microbial stratification in the gel beads. Unclassified Candidatus Brocadiaceae (major anammox bacteria) and Ignavibacterium (major ammonia oxidizing bacteria) enriched in the inside and outside layers of PVA gel beads, which benefited the synergetic cooperation of these bacteria and protecting them from environmental fluctuations. This study provides a promising solution for achieving a reliable PN/A process in mainstream wastewater treatment.In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 °C, pH 7; inoculums concentration (2.5% v/v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. MEK inhibitor side effects The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate.This work aimed to study the effects of biochar on methanogenesis and antibiotic resistance genes (ARGs) fate during anaerobic digestion (AD) of swine manure (SM) was investigated. Under the optimal biochar dosage of 5-10%, methane yield was significantly improved by 25%, ascribing to the enhancement of direct interspecies electron transfer (DIET). Biochar addition alleviated the need of cytochrome-c as interspecies electron connection components and enriched the microbes involved in DIET. Defluviitoga, Thermovirga and Cloacibacillus were dominant with biochar addition and might participate in DIET together with Methanothrix. The abundance of parC, tetX, blaCTX-M, blaTEM, aac(6')-Ib-cr, ermB and tetW were significantly reduced in biochar treatments and intI1 removal increased by 15% in B-5% than the control. Structural equation models demonstrated that intI1 (λ = 0.59, P less then 0.001) had the most standardized direct effects on ARGs, while biochar indirectly affected ARGs by changing intI1 (λ = 0.41, P less then 0.01) and microbial structure (λ = -0.24, P less then 0.05).In this study, the mixture of floral waste and cattle dung in different proportions was utilised to convert into vermicompost using earthworm Eisenia fetida. In the design of the experiment, the optimum amount of floral waste (1325 g) and cattle dung (500 g) was obtained for vermicompost using central composite design (CCD) and compared with the output of artificial neural network (ANN). The optimum proportions of vermicompost showed pH of 7.10, electrical conductivity of 3.39 mS/cm, total organic carbon of 34.01%, C N ratio of 13, phosphorous of 5.31 g/kg and potassium of 14.45 g/kg. This vermicompost was enriched with sufficient concentration of nutrients like potassium, sodium, phosphorous, and calcium, which are beneficial for the growth of the plants. The current study was based on comparing response surface methodology (RSM) and ANN for maturity parameters and the value of R2 in both the cases was near 1.

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