Carrvad9980

BACKGROUND Triple progressive thermopreconditioning (3PTP) may induce high Hsp-70 expression to maintain cardiac function. We suggest that 3PTP may reduce myocardial ischemia/reperfusion (I/R) injury during organ transplantation through Bag3/Hsp-70 mediated defense mechanisms. METHODS Male Wistar rats were divided into sham control group and 72 hours after 3PTP in a 42°C water bath (3PTP) group. Rats underwent 60 minutes of ischemia by occlusion of the left anterior descending coronary artery followed by 240 minutes reperfusion. CC220 Hemodynamic parameters, including the electrocardiogram, microcirculation, heart rate, left ventricular end-diastolic pressure (LVEDP), maximal rate of rise (+dp/dt) and fall (-dp/dt) in the left ventricular pressure for index of contraction and relaxation were determined. Myocardial infarct size was evaluated by the Evans blue-2,3,5-triphenyltetrazolium chloride method. 3PTP-induced protective mechanisms were determined by western blot and immunohistochemistry. RESULTS Cardiac I/R depressed cardiac microcirculation, induced S-T segment elevation and R-R and P-R interval elongation, increased infarct size associated with erythrocyte extravasation, leukocytes and macrophage/monocyte infiltration, granulocyte colony-stimulating factor (G-CSF), poly(ADP-ribose) polymerase 1 stain and TUNEL positive cells. However, 3PTP evoked significant cardio-protections against I/R injury, characterized by the increased +dp/dt value and the decreased elevated LVEDP, erythrocyte extravasation, leukocyte and macrophage/monocyte infiltration, G-CSF expression, poly(ADP-ribose) polymerase 1 expression, TUNEL-positive cells, fragmentation and infarct area. In addition, 3PTP increased Hsp-70 and Bag3 expression and decreased Bax/Bcl-2 ratio, but did not affect Beclin-1 and LC3-II/LC3-I ratio in the heart with I/R injury. CONCLUSIONS 3PTP therapies may through Bag3 upregulation alleviate I/R injury-induced left ventricular structural deterioration and dysfunction.BACKGROUND Fractures are a common and burdensome problem among kidney transplant recipients (KTRs). Proton pump inhibitors (PPIs) are frequently used after kidney transplantation and have been associated with increased fracture risk in the general population. This study aimed to determine whether PPI use is associated with incidence of major fractures in KTRs. METHOD Using the Wisconsin Allograft Recipient Database (WisARD), we identified 155 KTR with a major fracture that occurred at least 12 months after transplantation. Controls were selected using incidence density sampling. Use of PPIs and histamine 2-receptor antagonists (H2RA) during the year prior to the index date were identified. RESULTS A total of 155 cases were matched to 685 controls. Within one year prior to the index date, 68% of cases and 52% of controls used a PPI, and 16% of cases and 11% of controls used a H2RA. PPI use was associated with higher incidence of major fractures in unadjusted analysis (OR 2.4, 95% CI 1.6-3.5) and in adjusted analyses controlling for demographic and transplant-related covariates and use of corticosteroids, bisphosphonates, vitamin D and calcium supplements (OR 1.9, 95% CI 1.2-3.1). H2RA use was not associated with incidence of major fractures in adjusted analyses (OR 1.0, 95% CI 0.5-1.8). The associations between PPI use and fractures remained similar in analyses limited to spine and hip fracture. CONCLUSIONS Use of PPIs, but not H2RAs, is associated with higher risk of major fractures among KTRs. Clinicians should individualize PPI use in KTR, evaluating the risks and benefits of prescribing and continuing PPIs in KTRs.OBJECTIVE In Japan, a recent opinion poll in 2017 showed that 41.9% of the respondents wished to donate their organs but only 12.7% declared their intention to donate or to not do so. Therefore, it is important to explore measures to prompt more individuals to register their intention about organ donation. METHODS A field experimental questionnaire survey was conducted to confirm the effect of a prompt message for registering intentions for organ donation, which was communicated at a driver's license center. The study employed a prospective randomized control design. 7615 individuals visited the Tokyo Fuchu License Examination Center to update their driver's license and received leaflets including a message prompting organ donation registration and the questionnaire. Of the participants who received leaflets, 3224 respondents provided complete responses to the questionnaire (valid response rate 42.3%). Subsequently, a questionnaire survey was conducted to assess the participants' willingness to register for organ donation. A control condition and the following types of messages were used "Peer-framed," "Gain-framed," "Loss-framed," "Reciprocity-framed," and "Peer + Reciprocity-framed." RESULTS The reciprocity message emerged as a significant predictor of increase in immediate decision response. The loss-framed message was a significant predictor of decrease in no intention to register. CONCLUSIONS This study found that reciprocity and loss-framed messages promoted the readiness to register for organ donation among individuals from a Japanese urban area. Mandatory distribution of prompt messages at the every driver's license centers in Japan would be recommended.OBJECTIVE The goal of this study was to examine the independent and joint associations between anxiety and depression symptoms with the risk of heart disease. METHODS 30,635 participants from the CARTaGENE community cohort study in Quebec, without heart diseases at baseline, were included in the study. Baseline anxiety and depression symptoms were assessed via validated questionnaires. Survey data were linked with diagnostic codes from a public insurance database to examine incident heart disease during a 7-year follow-up period. Cox regression analyses were conducted comparing groups with high anxiety only, high depression only, comorbid anxiety and depression, and no/low symptoms of both on the risk of heart disease. Additional analyses examined anxiety and depression using continuous questionnaire symptom scores, data-driven comorbidity groups, and using diagnostic codes. Covariates included sociodemographic characteristics, health behaviours, diabetes, and hypertension. RESULTS In the main analyses, we found that whereas depression without anxiety symptoms was associated with an increased risk of heart disease (HR=1.