Sallinghaley5956

To analyse the correlation of dietary factors with haemoglobin concentration and body mass index.

This cross-sectional study was conducted in Shaheed Benazirabad district of the Sindh province in Pakistan from September 2015 to April 2016, and comprised students randomly selected from government schools. Data concerning dietary intake, such as meat, milk, egg and fruits, was noted. Baseline data was compared with haemoglobin and serum ferritin levels. Data was analysed using SPSS 20.

There were 1686 subjects aged 11-18 years; 946(56%) boys and 740(44%) girls. Dietary factors, such as meat, egg and fruits, showed significant association with haemoglobin, serum ferritin levels as well as with Body Mass Index and monthly household income (p<0.05). However, milk consumption had non-significant association with haemoglobin (9p>0.05).

Iron status in blood was found to be significantly affected by dietary intake, such as meat, milk, fruits and eggs.

Iron status in blood was found to be significantly affected by dietary intake, such as meat, milk, fruits and eggs.

To determine the effects of Wii Fit exer-gaming on balance confidence, quality of life and fall risk in elderly population.

The double-blind randomised control trial was conducted at Kulsum International Hospital, Islamabad, Pakistan, from July 31 to October 30, 2018, and comprised vitally stable, physically independent subjects of either gender aged 60 years and above, having no serious systemic disease. The subjects were randomly allocated into experimental group A and control group B using the concealed envelope method. After baseline assessment, group A received Wii fit gaming, while group B received balance training for 30 minutes twice a week for 8 weeks. Mycophenolic acid morpholinoethyl ester Activities balance confidence (ABC), European Quality of Life-5 Dimensions (EQ 5D-5L), timed up and go (TUG) and Fukuda were used to assess the impact of the intervention. Data was analysed using SPSS 21.

Of the 90 subjects, 83(92.2%) completed the study. There were 51(62%) males and 32 (38%) females; and 45(50%) in each of the two groups. Group A had 42(50.6%) subjects with 26(62%) males and 16(38%) females, and 41 were in group B, with 25(61%) males and 16(39%) females. Balance confidence improved in both groups, but it was significantly higher in group A (p<0.001). Mobility, self-care, usual activities, pain/discomfort, anxiety/depression as well as timed up and go and Fukuda showed significantly more improvement in group A compared to group B (p<0.05).

Wii fit was found to have the potential to improve balance confidence and quality of life, reducing the fall risk among the elderly.

IRCT20180417039344N1 by Iranian Registry of Clinical Trials.

IRCT20180417039344N1 by Iranian Registry of Clinical Trials.

To determine the effect of kangaroo mother care applied to the healthy newborns in the early postpartum period on breastfeeding.

The randomised controlled experimental study was conducted from June 1 to August 25, 2016, at a training and research hospital in Istanbul, Turkey, and comprised healthy newborns and their mothers equally divided into study group A and control group B. The newborns in group A were administered kangaroo mother care for 3 hours after birth, while group B newborns were cared for using the unit's standard postpartum procedure. The time the newborns in both the groups first started to breastfeed, the frequency of breastfeeding within the first 24 hours, the duration of their breastfeeding and suckling skills were evaluated. Data was analysed using the Number Cruncher Statistical System 2007.

There were 112 sets of newborns and their mothers; 56(50%) in each of the two groups. Group A newborns started suckling at the breast sooner, for longer duration, and more frequently compared to the newborns in group B (p<0.05). There was no difference between the groups in terms of initial suckling skills (p= 0.862), but those in group A were better at suckling 24 hours later (p=0.001).

Kangaroo mother care administered to healthy newborns immediately after birth encouraged the newborns to take to the breast sooner, more frequently, and for longer periods. It also increased their suckling skills.

ClinicalTrials.gov Identifier NCT04619459.

ClinicalTrials.gov Identifier NCT04619459.

To compare the effect of mobilisation with movement and Mulligan knee taping on anterior knee pain, hamstring flexibility and physical performance of the lower limb.

The randomised controlled trial was conducted from July to December, 2019, at the physical therapy department of Sahat Clinic, Rawalpindi, Pakistan, and comprise participants of both genders having patellofemoral pain who were randomised into mobilisation with movement group A and Mulligan knee taping group B. Both the groups were treated for 2 days per week for 2 consecutive weeks. Outcome was measured using the numeric pain rating scale, the Kujala pain rating scale, the active knee extension test and the time-up-and-go test. Assessments were taken at baseline, and at 2nd and 6th weeks post intervention. Data was analysed using SPSS 21.

Of the 34 participants, there were 17(50%) in each of the two groups. Overall, there were 6(17.6%) males and 28(82.4%) females with a mean age of 31.17±7.22 years. Group A showed significant improvement (p<0.0001) in terms of pain, while group B had better hamstring flexibility (p<0.0001). Both the groups showed a significant difference (p<0.0001) for all outcome variables post-intervention.

Mobilisation with movement was found to be more effective in the treatment of patellofemoral pain and associated knee functional performance.

Mobilisation with movement was found to be more effective in the treatment of patellofemoral pain and associated knee functional performance.Caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), COVID-19 is a virus-induced inflammatory disease of the airways and lungs that leads to severe multi-organ damage and death. Here we show that cellular lipid synthesis is required for SARS-CoV-2 replication and offers an opportunity for pharmacological intervention. Screening a short-hairpin RNA sublibrary that targets metabolic genes, we identified genes that either inhibit or promote SARS-CoV-2 viral infection, including two key candidate genes, ACACA and FASN, which operate in the same lipid synthesis pathway. We further screened and identified several potent inhibitors of fatty acid synthase (encoded by FASN), including the US Food and Drug Administration-approved anti-obesity drug orlistat, and found that it inhibits in vitro replication of SARS-CoV-2 variants, including more contagious new variants, such as Delta. In a mouse model of SARS-CoV-2 infection (K18-hACE2 transgenic mice), injections of orlistat resulted in lower SARS-CoV-2 viral levels in the lung, reduced lung pathology and increased mouse survival. Our findings identify fatty acid synthase inhibitors as drug candidates for the prevention and treatment of COVID-19 by inhibiting SARS-CoV-2 replication. Clinical trials are needed to evaluate the efficacy of repurposing fatty acid synthase inhibitors for severe COVID-19 in humans.Autoimmune diseases, including inflammatory bowel disease, multiple sclerosis and rheumatoid arthritis, have distinct clinical presentations but share underlying patterns of gut microbiome perturbation and intestinal barrier dysfunction. Their potentially common microbial drivers advocate for treatment strategies aimed at restoring appropriate microbiome function, but individual variation in host factors makes a uniform approach unlikely. In this Perspective, we consolidate knowledge on diet-microbiome interactions in local inflammation, gut microbiota imbalance and host immune dysregulation. By understanding and incorporating the effects of individual dietary components on microbial metabolic output and host physiology, we examine the potential for diet-based therapies for autoimmune disease prevention and treatment. We also discuss tools targeting the gut microbiome, such as faecal microbiota transplantation, probiotics and orthogonal niche engineering, which could be optimized using custom dietary interventions. These approaches highlight paths towards leveraging diet for precise engineering of the gut microbiome at a time of increasing autoimmune disease.Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone-phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photostimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice.The discovery and clinical implementation of immune-checkpoint inhibitors (ICIs) targeting CTLA4, PD-1 and PD-L1 has revolutionized the treatment of cancer, as recognized by the 2018 Nobel Prize for Medicine and Physiology. This groundbreaking new approach has improved the outcomes of patients with various forms of advanced-stage cancer; however, the majority of patients receiving these therapies, even in combination, do not derive clinical benefit. Further development of agents targeting additional immune checkpoints, co-stimulatory receptors and/or co-inhibitory receptors that control T cell function is therefore critical. In this Review, we discuss the translational potential and clinical development of agents targeting both co-stimulatory and co-inhibitory T cell receptors. Specifically, we describe their mechanisms of action, and provide an overview of ongoing clinical trials involving novel ICIs including those targeting LAG3, TIM3, TIGIT and BTLA as well as agonists of the co-stimulatory receptors GITR, OX40, 41BB and ICOS. We also discuss several additional approaches, such as harnessing T cell metabolism, in particular via adenosine signalling, inhibition of IDO1, and targeting changes in glucose and fatty acid metabolism. We conclude that further efforts are needed to optimize the timing of combination ICI approaches and, most importantly, to individualize immunotherapy based on both patient-specific and tumour-specific characteristics.

This study aimed to evaluate neoadjuvant chemotherapy (NACT) for locally advanced stage cervical carcinoma.

Data of 43 patients with locally advanced cervical carcinoma who had NACT were reviewed. NACT protocols implemented included cisplatin/5-fluorauracil, cisplatin/UFT, and carboplatin/paclitaxel. After NACT, the patients were re-examined, and patients who had a tumor size ≤40 mm underwent Piver-Rutledge type III radical hysterectomy, while other patients received radiotherapy. Following NACT, clinical responses were assessed according to the criteria of the World Health Organization.

The mean age of the patients was 49.4 years, and the median follow-up duration was 48 (range, 5-228) months. The median tumor sizes were 50 and 30 mm before and after NACT, respectively. Complete clinical response was observed in 4 (9.3%) patients, partial clinical response in 8 (18.6%), and pathologic complete response in 3 (6.9%). Stable disease was noted in 30 (69.9%) patients and progression in 1 (2.3%) patient. After NACT, 31 patients have undergone radical surgical procedures.