Maddennorup7050
In this study, we also observed that male patients were slightly more susceptible to BRAF
mutation, and this mutation was predominant in patients of the age group<40years. However, these parameters did not translate to statistical significance.
The data demonstrate that BRAF
mutation is observed significantly more often in intra-axial brain tumor patients, which can serve as baseline information for further research on genetic alteration that occurs during brain tumor progression in the Malaysian population.
The data demonstrate that BRAFV600E mutation is observed significantly more often in intra-axial brain tumor patients, which can serve as baseline information for further research on genetic alteration that occurs during brain tumor progression in the Malaysian population.Prostate cancer is one of the leading cancers in men, and new approaches are needed for its treatment. The aim of this study was to investigate the effect of co-administration of naturally occurring flavone apigenin and doxorubicin to androgen-insensitive prostate cancer cells.
The effect of the treatment on survival and migration of human PC3cells was evaluated by MTT and scratch assay, respectively. Apoptosis and cell cycle distribution were detected by image-based cytometry. mRNA and protein expression were determined by real-time quantitative polymerase chain reaction and Western blot, respectively.
Apigenin and doxorubicin dose-dependently inhibited cell survival, and co-administration of both agents significantly induced cell death via upregulating the mRNA expression of caspases, Bax and cytochrome c, and downregulating Bcl-XL. Combination therapy caused cell cycle arrest by upregulating the expression of p21and p27. The treatment modality inhibited cell migration via downregulating Snail, Twist and MMPs in which doxorubicin was ineffective. Apigenin dephosphorylated Akt strongly, significantly suppressed ERK phosphorylation, and increased PTEN expression 4.5-fold. The combination of apigenin and doxorubicin inhibited PI3K and AKT phosphorylation more strongly than a single administration.
Our data indicate that a combination of the natural flavone apigenin with doxorubicin might have a potential in treatment of castration-resistant prostate cancer.
Our data indicate that a combination of the natural flavone apigenin with doxorubicin might have a potential in treatment of castration-resistant prostate cancer.
The bladder cancer is immunogenic, and neoantigens generated by tumor cells trigger a notable immune response in the host. On the other hand, multiple immune escape mechanisms allow for avoiding the recognition by the host immune system. Toll-like receptor type 4and inflammatory cytokines play major role in the immune response to bladder cancer.
To assess the expression of TLR4and the genes of major inflammatory cytokines in tumor cells and in unaffected tissue of the bladder.
The pairs of samples from the urinary bladder tumor and unaffected adjacent tissue were obtained from 50surgically treated patients with bladder cancer. The level of expression of TLR4, TGF-β1, INF-γ, TNF-α genes was evaluated by real-time polymerase chain reaction.
Bladder cancer cells are characterized by lower expression levels of TLR4, TGF-β1, INF-γ, TNF-α as compared to unaffected tissue. In patients with recurrent cancer, expression of TLR-4and cytokines does not change both in tumor and in unaffected tissue of the bladder. Expression of TLR4is identically low both in low- and high-grade cancer. Expression levels of the INF-γ and TNF-α are remarkably low in muscle-invasive cancer compared to the unaffected bladder tissue. The level of TGF-β1in bladder cancer is comparable to the unaffected tissue of the bladder, while in the intact and metastatic lymph nodes it is significantly upregulated.
Bladder cancer tissue differs from the unaffected part of the bladder wall in the level of TLR4, TGF-β1, INF-γ, TNF-α expression.
Bladder cancer tissue differs from the unaffected part of the bladder wall in the level of TLR4, TGF-β1, INF-γ, TNF-α expression.
To study the peculiarities of ecological relationships of breast cancer (BC) cell lines MCF-7, BT-474and MDA-MD-231under co-culturing conditions.
Three BC cell lines luminal A- MCF-7, luminal B- BT-474and triple-negative- MDA-MD-231were co-cultured pairwise. Immunocytochemistry was used to differentiate the cell lines in the wells. The effect of the cell-free culture medium on the growth rate of the alternate cell line in the pair was also evaluated.
It was shown that when BT-474cells were co-cultured with MCF-7and BT-474cells were co-cultured with MDA-MD-231, two types of ecological interactions could be observed commensalism and amensalism, respectively. While the cells do not interact with each other in contact, the supernatants of single cultures of MCF-7and MDA-MD-231exert the same effect on BT-474as co-cultivation of BT-474with these cells.
The paracrine mechanism of intercellular interaction between different human BC cell lines has been demonstrated. The models used in population ecology can be applicable to identify the types of interaction between cell lines.
The paracrine mechanism of intercellular interaction between different human BC cell lines has been demonstrated. The models used in population ecology can be applicable to identify the types of interaction between cell lines.
Uterine leiomyoma (UL) is the most common benign neoplasm of the uterus. It is still unknown surely what exactly initiates transformation of the uterine myometrial cells into UL.
To study the effect of the TP53gene variants on the risk of development and clinical features of UL.
Case-control study was performed using molecular genetic analyses of variants rs1042522 (119G>C) and rs1625895 (13494G>A) of TP53gene in patients with UL and comparison group of healthy women.
Investigated TP53gene variants were not associated with the risk of UL development. The patients with the 13494GG genotype (rs1625895) had significantly more often subserous UL (р< 0.05). In patients with heterozygous variant of TP53- 13494GA genotype (rs1625895) intramural UL was observed (р< 0.05).
The rs1625895 (13494G>A) variant of TP53gene was associated with UL localization. The identified dependence of the UL localization on the TP53gene variant could be useful for personalized approach to treatment.
A) variant of TP53 gene was associated with UL localization. The identified dependence of the UL localization on the TP53 gene variant could be useful for personalized approach to treatment.
It is known that interactions between tumor and endothelial cells have a significant influence on the growth and metastasis of malignant tumors.
To study the reciprocal effect of Lewis lung carcinoma (LLC) and endothelial cells on the growth rate of each other upon their co-cultivation in vitro and to assess the contribution of such tumor/endothelial cell crosstalk to in vivo LLC growth and metastasis.
Two variants of Lewis lung carcinoma cells, high-metastatic (LLC) and low-metastatic (LLC/R9), and murine aorta endothelial cell line (MAEC) were used. Kinetics of tumor cell growth in vitro and in vivo, electrokinetic properties of tumor cells and their adhesion to endothelial monolayer, and the number of tumor and endothelial viable cells after 1-day contact or non-contact co-cultivation were estimated.
LLC/R9 had significantly higher growth rate in vivo (as opposed to in vitro) than LLC. However, the number and volume of lung metastatic lesions in LLC/R9-bearing mice were 4.5-fold (p < 0.05) and 3 such a subpopulation was absent and 19% of cells had a surface charge < 5 C/m
. The number of LLC cells that adhered to the monolayer of endothelial cells was by 65% (p < 0.05) higher than that of LLC/R9 cells.
Obtained data demonstrated that the tumor/endothelial cell relationships might reflect the features of tumor growth and metastasis of a malignant tumor.
Obtained data demonstrated that the tumor/endothelial cell relationships might reflect the features of tumor growth and metastasis of a malignant tumor.Chronic renal failure is one of the most challenging complications after the completed surgical treatment for renal cell cancer. In 2016, a grading system of tumorous renal involvement was developed, referred to as NCIU nephrometry. However, the systematic parameter to reflect the functional status of the functional renal parenchyma is defined by tumor volume only, with no regard for spatial disposition of the segment(s) where the tumor is located. Our research team decided to improve the NCIU nephrometry system by developing and testing a modified formula for calculation of creatinine clearance, which makes allowance for spatial disposition of tumor within the kidney. We performed numerical computations and analysis of changes in functional status of renal parenchyma depending on coordinate-based spatial location of the tumor in order to augment the existing NCIU nephrometry scale; Matlab, a specialized software package was used as a principal instrument to calculate the number of nephrons and functional renal parenchyma depending on the coordinate-based position of the mass center of the tumor and tumor volume. This model was shown to create a feasible opportunity to increase the percentage of organ-sparing procedures for renal cell cancer and to reduce the incidence/progression of chronic renal failure in these patients.
The expression of the CXCL12chemokine and its receptor CXCR4in the stromal component of the tumor plays an important role in tumor cell migration, proliferation, inhibition of apoptosis and determination of invasive and metastatic potential of malignant neoplasms of various genesis. The significance of CXCL12and CXCR4expression in endometrial tumor cells for cancer progression is not fully understood.
To evaluate the content of CXCL12
-fibroblasts and expression of CXCL12and CXCR4in endometrial cancer cells, depending on the tumor stage.
Surgical material of 45patients with endometrioid carcinoma of the endometrium (ECE) of the stages I-II and III was studied using morphological and immunohistochemical methods.
In ECE of stage I-II CXCR4expression was lower (43.3± 4.2%) while CXCL12expression was higher (33.6± 2.4%) compared with the corresponding indices in ECE of stage III (63.6± 3.5%, 24.5± 1.9%, respectively, p< 0.05). In ECE of stage III, high expression of CXCR4 (> Me) and low CXCL12 (&bined effect of these two factors.Brain metastases of solid tumors are the most common intracranial neoplasms in adults. We provide a short overview of the role of the blood-brain barrier in the pathogenesis of breast cancer brain metastases, and the effectiveness of systemic anticancer therapy in the treatment of such patients.
The objective of this study is to report the therapeutic results of the preservation strategy in locally advanced laryngeal cancers.
Between January 2008and December 2015, 24patients with locoregional advanced non-metastatic laryngeal cancer (T2-4/N0-2) were collected retrospectively. EGFR inhibitor Different therapeutic sequences were used either induction chemotherapy followed by concurrent chemoradiotherapy or induction chemotherapy followed by radiotherapy or concurrent chemoradiotherapy or radiotherapy alone.
The objective response rate was 85.7%. Overall survival rates at 1year, 3years and 5years were 91.3%, 80.2% and 53.5%, respectively. Administration of induction chemotherapy did not improve overall survival. The 1-year overall survival was 83.3% in the induction chemotherapy group vs 94.1% for those who did not received induction chemotherapy (p= 0.7).
Our study showed the feasibility of this preservation strategy in clinical practice, with acceptable term toxicity.
Our study showed the feasibility of this preservation strategy in clinical practice, with acceptable term toxicity.
