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This work was aimed to explore the effects of ultrasound as an emulsification method on the properties of pork myofibrillar protein (MP) and fat mixed gel under different ratios (w/v, 151, 101, 51, 11, 15, 110 and 115). Results indicated that sonication decreased the hardness, springiness and water holding capacity of gels with the ratios higher than 51, while increased these properties with the ratio at 115. Sonication also increased the storage modulus value of samples with the ratios lower than 110 during 58 to 80 ℃. The effects of ultrasound emulsification on ultrastructure, surface hydrophobicity and free sulfhydryl group contents of samples were different with the changes of ratios. The absolute zeta potential value was increased of all samples after sonication. MP were combined with fat particles after sonication. Our results demonstrate that ultrasound emulsification can be used to improve the properties of higher fat MP gel.This study investigated the individual and combined effects of ultraviolet A (UV-A) and far-red (FR) light irradiation on the biomass, phytochemical accumulation, and antioxidant capacity of two lettuce cultivars in an artificial light plant factory. UV-A supplementation yielded a smaller leaf area and reduced biomass and nitrate content. In contrast, it improved the chlorophyll, soluble protein, soluble sugar, vitamin C, flavonoid, polyphenol, and anthocyanin contents and the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging rate. FR irradiation resulted in a larger leaf area, whereas the lettuce biomass remained unchanged. Irradiation with both UV-A and FR light exhibited the most remarkable effect on leaf expansion and biomass, but reduced the phytochemical contents. A significant interaction between the cultivar and supplemented light was observed for most plant parameters.Here, an efficient method for synthesizing carbon dots (CDs) using a deep eutectic solvent (DES) was developed. To investigate the influence of different DESs on the quantum yield of CDs, different hydrogen-bonding acceptors (HBAs) and hydrogen-bonding donors (HBDs) were used to synthesize the DES and prepare CDs. Using Sophora flavescens Aiton as precursor, CDs were prepared using choline chloride (ChCl)/urea based DES as reaction media and doping agent in the presence of water. Selleck PT-100 The CDs showed strong blue fluorescence and were further modified with polyethyleneimine (CDs@PEI). The fluorescence intensity of CDs@PEI was selectively quenched by myricetin with a limit of detection (LOD) of 10 nM. Furthermore, CDs@PEI was used to analyze myricetin in the extracts that were fluorescent by DES with satisfactory performance of Abelmoschus manihot (Linn.) Medicus flowers, vine teas and blueberries. Finally, the bio-imaging application of CDs@PEI was tested and the results confirmed its potential application in bio-imaging.Innovation in products and processes, traceability, food security and quality control are inherent challenges in agri-food sector. Trends in wine production are focused on obtaining natural wines with less chemical intervention. Following this goal, a low cost miniaturized, easy-to-use and highly automated microanalyzer to monitor total potassium in winemaking processes is presented. The microsystem monolithically integrates microfluidics as well as a potentiometric detection system and does not require any sample pretreatment. The analytical features provided are a linear range from 250 to 4000 mg L-1 K+, covering all the concentrations expected in must and wine samples, a detection limit of 75 ± 12 mg L-1 K+, and an adequate reproducibility and repeatability. Sample throughput is calculated at 20 h-1 with a waste volume generation lower than 4 mL per analysis. The microsystem lifetime is at least 4 months. Different wine and grape juice samples have been analyzed reaching outstanding results.Charantin, a steroidal glycoside, exists as a mixture of stigmasterol glucoside (STG) and β-sitosterol glucoside (BSG) in the fruits of Momordica charantia. Charantin has anti-diabetic activity comparable to insulin. The present work discusses a method for separation of components of charantin namely STG and BSG by simple extraction technique followed by preparative HPLC. The identity of separated components was established by chromatographic as well as spectral techniques. Also reversed phase HPLC-DAD method was developed and validated for estimation of STG and BSG present in fruits of Momordica charantia. The method used C18 column (75 mm × 4.6 mm, 3.5 µm) as stationary phase and methanol water (9802, v/v) as mobile phase. Retention times of STG and BSG were found to be 10.707 min and 11.870 min, respectively. The validated method was applied to evaluate content of these components in different extracts and some commercial herbal formulations containing fruits of Momordica charantia.Determination of food doneness remains a challenge for automation in the cooking industry. The complex physicochemical processes that occur during cooking require a combination of several methods for their control. Herein, we utilized an electronic nose and computer vision to check the cooking state of grilled chicken. Thermogravimetry, differential mobility analysis, and mass spectrometry were employed to deepen the fundamental insights towards the grilling process. The results indicated that an electronic nose could distinguish the odor profile of the grilled chicken, whereas computer vision could identify discoloration of the chicken. The integration of these two methods yields greater selectivity towards the qualitative determination of chicken doneness. The odor profile is matched with detected water loss, and the release of aromatic and sulfur-containing compounds during cooking. This work demonstrates the practicability of the developed technique, which we compared with a sensory evaluation, for better deconvolution of food state during cooking.Contemporary systems for oocyte retrieval and culture of both cattle and human embryos are suboptimal with respect to pregnancy outcomes following transfer. In humans, chromosome abnormalities are the leading cause of early pregnancy loss in assisted reproduction. Consequently, pre-implantation genetic testing for aneuploidy (PGT-A) is widespread and there is considerable interest in its application to identify suitable cattle IVP embryos for transfer. Here we report on the nature and extent of chromosomal abnormalities following transvaginal follicular aspiration (OPU) and IVP in cattle. Nine sexually mature Holstein heifers underwent nine sequential cycles of OPU-IVP (six non-stimulated and three stimulated cycles), generating 459 blastocysts from 783 oocytes. We adopted a SNP-array approach normally employed in genomic evaluations but reanalysed (Turner et al., 2019; Theriogenology125 249) to detect levels of meiotic aneuploidy. Specifically, we asked whether ovarian stimulation increased the level of aneuploidy in either trophectoderm (TE) or inner-cell mass (ICM) lineages of blastocysts generated from OPU-IVP cycles.

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