Zimmermanngoodman6060
Human iPSC cell line FAMRCi010-A was generated from a patient with restrictive cardiomyopathy carrying FLNC p.Gly2011Arg genetic variant. Patient-specific peripheral blood mononuclear cells were reprogrammed using non-integrative Sendai viruses containing OCT4, SOX2, KLF4, and CMYC. FAMRCi010-A was generated and characterized through the study. The reported iPSC line could be useful tool for in vitro modeling of FLNC-associated cardiomyopathies.
The actual role of neutrophils and neutrophil extracellular traps (NETs) in the course of cancer has not been clearly defined. The aim of this study was to determine the clinical usefulness of NETs biomarkers in saliva in confrontation with the blood serum and tumor tissue as a potential prognostic and therapeutic target in patients with oral squamous cell carcinoma (OSCC).
Expression of myeloperoxidase (MPO), and histones H2A, H2B, H3 in the tumor tissue, was investigated using immunohistochemistry. The expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits p47-phox, p67-phox (neutrophil cytosolic factor 2, NCF2) and panRac, as well as citrullinated histone H3 (CitH3) in peripheral blood neutrophil lysates, was assessed via Western blot. ELISA tests were employed to measure the concentrations of circulating free DNA (cfDNA) and MPO in saliva only, and NOX1, NCF2, DNASE1 in saliva and serum.
Extracellular expression of MPO and histones was localized within tumor tissue. Significantly lower expression of p67-phox, panRac, and CitH3 was determined in OSCC patients. Considerably lower concentrations of NOX1, NCF2, and DNASE1 in the saliva samples of cancer patients were observed. However, the levels of NOX1, NCF2, and DNASE1 in the serum of patients with cancer were substantially higher.
The results obtained from the saliva of cancer patients suggest an impairment of the immunological homeostasis within the oral cavity related to NET formation, the causes of which should be sought in deficient activation of NADPH oxidase.
The results obtained from the saliva of cancer patients suggest an impairment of the immunological homeostasis within the oral cavity related to NET formation, the causes of which should be sought in deficient activation of NADPH oxidase.In bacteriophage therapy, the combination of different phages into a single cocktail is of critical importance to overcome the narrow host range of single phage isolates. Today, the design of therapeutic cocktails is often akin to a black box and relies largely on intuition and (pre-)availability of isolates in local collections. Here we show that straightforward host range analysis can disclose design rules and we propose to apply/translate a data mining approach, historically applied in the field of marketing ('shopping cart analysis') to explore patterns in phage combinations. The technique is broadly applicable to host range datasets and can serve in combination with other molecular-based approaches to propose rationales for phage cocktail design.
Total sleep deprivation has a visible impact on subjective facial appearance. However, there is a lack of knowledge on how moderate sleep restriction objectively impairs skin quality and facial aspect.
Twenty-four healthy good-sleeping women, aged 30-55, volunteered for this study on the impact of sleep restriction (SR) on their facial skin. SR was limited to 3h per night for 2 consecutive nights. PORCN inhibitor We assessed the following parameters at the same time of day, before and after SR sebumetry (Sebumeter SM 815), hydration (Corneometer CM 825), trans-epidermal water loss (Tewameter TM 210), biomechanical properties (Cutometer MPA 580), pH (PH-meter 900), desquamation quantification (D-Squameter and microscopy), and image analysis (ColorFace - Newtone Technologies). We also obtained skin samples (swab) for malondialdehyde quantification (MDA).
We observed that some skin parameters are significantly associated with SR in both the morning and afternoon, including lower hydration (p<0.001), increased trans-epidermal water loss (PIE) (p<0.001), and decreased extensibility (Uf; p=0.015) and viscosity (Uv; p<0.001) of the skin. The average pH increased from 4.8 (±0.2) to 4.9±0.4; p<0.001. For face photography, brightness and saturation also significantly decreased with SR in mornings and afternoons (p<0.001 for all tests). Finally, we observed a significant decrease in isolated corneocytes after desquamation associated with SR (p<0.001 for all tests). SR was also associated with significantly increased MDA levels (p<0.001 for all tests).
Two nights of SR significantly altered the skin and facial appearances in our test group of typically good-sleeping women.
Two nights of SR significantly altered the skin and facial appearances in our test group of typically good-sleeping women.
This study aimed to investigate the association between adenotonsillectomy (T&A) and serial changes in growth in children through a population-based nationwide study as well as the national health insurance service (NHIS) database.
Propensity score (PS) matched children with and without T&A were selected in NHIS database, which includes all individuals born in Korea in 2008-2009. Serial changes of BMI and height were compared in children with and without T&A, and growth changes depending on the time of operation were also considered. The outcomes were differences in age-/sex-standardized BMI (BMI-z) and height (height-z) between the groups. Changes in BMI-z and height-z were further analyzed according to the timing of operation.
Of 919,707 individuals born in Korea in 2008-2009, 3172 children were included in the operation group and 31,663PS-matched children were included in the control group. T&A was related to increased weight and height in the operation group than in the control group. At 66-71 months of age, BMI-z and height-z were 0.41 (0.02) and 0.42 (0.02), respectively, in the operation group and 0.18 (0.01) and 0.35 (0.01), respectively, in the control group. On adjustment with preoperative BMI-z, more weight gain was noted in the operation group (p<0.001). Shortly after T&A, BMI-z increased significantly in the operation group; a significant increase in height-z was observed more than 1 year after T&A.
Children who underwent T&A tended to experience a growth spurt; when surgical intervention such as T&A is required, care should be taken to minimize or reverse the anticipated weight gain.
Children who underwent T&A tended to experience a growth spurt; when surgical intervention such as T&A is required, care should be taken to minimize or reverse the anticipated weight gain.
The association of obstructive sleep apnea (OSA) with bradycardia is not well-characterized, which may confer significant morbidity and mortality if left untreated. We sought to clarify the prevalence of comorbid OSA and bradycardia, and the effect of continuous positive airway pressure (CPAP) therapy on bradycardia outcomes.
We systematically searched four electronic databases (PubMed, Embase, Cochrane Library, Scopus) for randomized or observational studies reporting the co-prevalence of sleep apnea and bradycardia or evaluated the use of CPAP on the incidence of bradycardias. We used random-effects models in all meta-analyses and evaluated heterogeneity using I
.
We included 34 articles from 7204 records, comprising 4852 patients. Among patients with OSA, the pooled prevalence of daytime and nocturnal bradycardia were 25% (95% CI 18.6 to 32.7) and 69.8% (95% CI 41.7 to 88.2) respectively. Among patients with bradycardia, the pooled prevalence of OSA was 56.8% (95% CI 21.5 to 86.3). CPAP treatment, compared to those without, did not significantly reduce the risk of daytime (two randomized trials; RR 0.50; 95% CI 0.11 to 2.21) or nocturnal bradycardia (one randomized-controlled trial and one cohort study; RR 0.76; 95% CI 0.48 to 1.20).
This meta-analysis demonstrates a high comorbid disease burden between OSA and bradycardia. Future research should explore the treatment effect of CPAP on bradycardia incidence, as compared to placebo.
This meta-analysis demonstrates a high comorbid disease burden between OSA and bradycardia. Future research should explore the treatment effect of CPAP on bradycardia incidence, as compared to placebo.Prospective memory (PM) allows us to form intentions and execute them in the future. Successful retrieval of prospective intentions depends on adequate context monitoring and disengagement from the ongoing task. These processes are also central in predicting incoming language information and guiding language production in bilinguals. We investigated if different bilingual experiences (early/late bilinguals, monolinguals) modulate performance in PM tasks that varied in attentional requirements (focal vs. non-focal). Behavioural and event-related potential (ERP) results indicated that early bilinguals differed from late bilinguals and monolinguals in how they performed the prospective task. Specifically, they showed larger differences between the ongoing activity and the prospective task in the N300 and P3b components when performing the more difficult non-focal PM task, indicating that they engaged in monitoring/updating to adapt to the task's demands. These differences were not observed in late bilinguals and monolinguals, suggesting that prospective processing is dependent on the bilingual experience.Safe, effective, cost-effective, easy feasible and low-waste decontamination technologies are fundamental importance from environmental and radiation protection aspects. In this study the effectiveness of AP-CITROX decontamination technology of Inconel alloy 690 was investigated. Non-radioactive representative metal samples were formed to test of decontamination technology and the clear-, the corroded-, the decontaminated layer were analysed electrochemically. The results indicate that the passivation step of the technology was not completed.Photo-neutron production in electron accelerator near the threshold energy has been studied by Monte Carlo simulation using the FLUKA code. A pencil beam of 10 MeV electron was incident normally on W, Ta, Pb and Bi targets and a CR-39 detector attached to the target was used as scoring region. In the simulation, photon and neutron spectra, yield and their spatial distribution, fast neutron fluence above 100 keV, total dose and neutron dose deposition were estimated for these metallic targets. The photon emission was found to be forward peaked whereas the neutron emission was isotropic in nature. The photon yield was found to be 5 to 6 orders of magnitude higher than that of neutrons. In W and Ta, the photon yield is maximum for 1.5 mm thickness whereas for Pb and Bi, the photon yield is highest at 2 and 2.5 mm respectively. The total neutron yield was highest for W and lowest for Pb whereas highest number of fast neutrons above 100 keV was for W followed by Bi, Pb and Ta. Production of significant number of fast neutrons above 100 keV suggests the possibility of the use of CR-39 detector for measurement of these neutrons. The total dose deposition was found to be highest for Bi followed by Pb, Ta and W whereas the neutron dose equivalent was highest for W followed by Pb, Bi and Ta. This simulation study will be useful for neutron dosimetry, estimation of source term, implementation of CR-39 for measurement and other radiation protection aspects in the vicinity of an electron accelerator.
