Mahmoudgutierrez7941

The clinical diagnosis of synucleinopathies, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA), is challenging, especially at an early disease stage, due to the heterogeneous and often non-specific clinical manifestations. The discovery of reliable specific markers for synucleinopathies would consequently be of great aid to the diagnosis and management of these disorders. Real-Time Quaking-Induced Conversion (RT-QuIC) is an ultrasensitive technique that has been previously used to detect self-templating amyloidogenic proteins in the cerebrospinal fluid (CSF) and other biospecimens in prion disease and synucleinopathies. Using a wild-type recombinant α-synuclein as a substrate, we applied RT-QuIC to a large cohort of 439 CSF samples from clinically well-characterized, or post-mortem verified patients with parkinsonism or dementia. Of significance, we also studied patients with isolated REM sleep behavior disorder (iRBD) (n = 18) and pure autonomic failure (Plein seeding activity in MSA seems to support the current view that MSA and LBD are associated with different conformational strains of α-synuclein.The catabolic activity and structural diversity of soil bacteria covered by five different halophytic vegetation types in the Yellow River Delta affected by long-term salinization were studied using Biolog-Eco technology. The result showed that soil quality, the diversity, and catabolic activity of the bacterial community of mildly salt-tolerant vegetation (Imperata cylindrical (L.) Beauv. and Apocynum venetum L.) were significantly higher than those of the bacterial community of highly salt-tolerant vegetation (Suaeda salsa (L.) Pall., Aeluropus sinensis (D.) Tzvel.), while these values were lowest for bacterial communities in bare land. The operational taxonomic units (OTUs) and diversity indexes of soil bacteria covered by Aeluropus sinensis were higher than those of soil bacteria covered by other types of vegetation, while those of soil bacteria covered by bare land were lowest. Principal component analysis (PCA) of the carbon source utilization capacity of the soil bacterial communities showed that organic acids, polymers, and amino acids were sensitive carbon sources that enabled study of the diversity of carbon metabolic functions in soil bacterial communities. And redundancy analysis (RDA) showed that D-galacturonic was significantly positively correlated with Verrucomicrobia, which further demonstrated the effect of organic acid carbon sources on metabolic functional diversity of soil bacterial communities in the Yellow River Delta.PURPOSE To investigate the impact of menstrual cycle on patients undergoing gynecological endoscopic surgery. PATIENTS AND METHODS 220 patients scheduled for gynecological endoscopic surgery under general anesthesia were selected. The patients were divided into three groups based on 3 phases of menstrual cycle which are the follicular phase (Group F), ovulatory phase (Group O) or luteal phase (Group L). It is based on their duration of menstruation and the last day of menstrual bleeding from the date of surgery. Primary outcomes were the incidences of early and late postoperative nausea and vomiting (PONV) in the three patient groups. Preoperative venous blood was taken to determine the estrogen and progesterone levels of the patients. RESULTS A total of 207 patients were enrolled. The incidence of early PONV was highest in group O (22.22% vs 43.33% vs 17.86%, P  less then  0.01). Multivariate logistic regression showed that menstrual cycle (P  less then  0.01) and sufentanil dosage (P  less then  0.05) were independent risk factors for early PONV, menstrual cycle (P = 0.03) and intraoperative hypotension (P = 0.03) were independent risk factors for late PONV. After the propensity matching, the incidences of early and late PONV in group O were both higher than that in other two groups (19.23% vs 44.68% vs 16.90%, P  less then  0.01; 53.80% vs 72.34% vs 45.07%, P = 0.01). CONCLUSION The incidence of PONV after gynecological endoscopic surgery was different in patients with different menstrual cycles, with the highest incidence in ovulation.The indiscriminate use of pesticides has caused several damages to the environment, in particular the pollution of water reservoirs, so that this has motivated the development of techniques to minimize its consequences. One of the main surface water pollutants is glyphosate, which is a widely used herbicide for weed control. Therefore, in this work, computational simulations were used with density functional theory and molecular dynamics to theoretically verify if C60 fullerene is capable of adsorbing glyphosate in aqueous media. As a result, we showed through the adsorption energies, molecular dynamics methods, and infrared absorption that C60 can adsorb glyphosate molecules in at least three distinct configurations, either in vacuum or in water, which theoretically indicates it as a good candidate for removal of this herbicide from water by nanotechnology techniques.In the current SARS-CoV-2 pandemic there are many questions regarding the safe treatment of patients with inflammatory rheumatic diseases. Many of these questions cannot yet be answered on an evidence-based basis and this does not make patient care easy. The German Society for Rheumatology (DGRh) hopes that these initial recommendations will provide support for specific issues in the care of patients with inflammatory rheumatic diseases in view of the current threat posed by SARS-CoV-2. In order to take advantage of the dynamic worldwide gain in knowledge for our patients, the recommendations will be updated regularly. The updated versions of the recommendations are deposited on the homepage of the DGRh.The study assessed changes in the total 16 PAH (polycyclic aromatic hydrocarbon) content of soil which occurred in 1998-2009, during a multi-annual, manure-mineral and mineral fertilisation experiment, carried out in Bałcyny near Ostróda (Poland), according to a design unchanged since 1986 The study focused on the impact of multi-annual, diversified mineral fertilisation (N, P, K, Mg and Ca) compared to manure applied every two years at a dose of 40 t/ha. The four plants used in the crop rotation included sugar beet, spring barley, maize and spring wheat. The content of the total 16 polycyclic aromatic hydrocarbons was significantly higher in the manure-fertilised soil than in the soil fertilised with mineral fertilisers only. Under the regular manure fertilisation conditions, liming of the soil significantly increased the total 16 PAH content, and the lowest dose of nitrogen significantly decreased its PAH content. The lowest nitrogen dose had an opposite effect in the soil fertilised with mineral fertilisers only, where it caused a significant increase in the content of the PAHs. However, the increased doses of nitrogen also resulted in an increase in the PAH content in the soil fertilised with manure and without this fertiliser.OBJECTIVES Computed tomography (CT) pulmonary angiography is widely used in patients with suspected pulmonary hypertension (PH). However, the diagnostic and prognostic significance remains unclear. The aim of this study was to (a) build a diagnostic CT model and (b) test its prognostic significance. METHODS Consecutive patients with suspected PH undergoing routine CT pulmonary angiography and right heart catheterisation (RHC) were identified. Axial and reconstructed images were used to derive CT metrics. this website Multivariate regression analysis was performed in the derivation cohort to identify a diagnostic CT model to predict mPAP ≥ 25 mmHg (the existing ESC guideline definition of PH) and > 20 mmHg (the new threshold proposed at the 6th World Symposium on PH). In the validation cohort, sensitivity, specificity and compromise CT thresholds were identified with receiver operating characteristic (ROC) analysis. The prognostic value of the CT model was assessed using Kaplan-Meier analysis. RESULTS Between 2012 and 2016, 491 patients were identified. In the derivation cohort (n = 247), a CT model was identified including pulmonary artery diameter, right ventricular outflow tract thickness, septal angle and left ventricular area. In the validation cohort (n = 244), the model was diagnostic, with an area under the ROC curve of 0.94/0.91 for mPAP ≥ 25/> 20 mmHg respectively. In the validation cohort, 93 patients died; mean follow-up was 42 months. The diagnostic thresholds for the CT model were prognostic, log rank, all p  less then  0.01. DISCUSSION In suspected PH, a diagnostic CT model had diagnostic and prognostic utility. KEY POINTS • Diagnostic CT models have high diagnostic accuracy in a tertiary referral population of with suspected PH. • Diagnostic CT models stratify patients by mortality in suspected PH.Mercury is the only metal that remains in liquid form at the room temperature. It is a very toxic metal and even short-term exposure can lead to poisoning. Mercury intoxication can affect many systems such as skin, cardiovascular, genitourinary, central and peripheral nervous, respiratory, and musculoskeletal system. Consequently, the diagnosis of mercury intoxication can be challenging due to its non-specific and multisystemic presentation. Herein, we report five pediatric cases with mercury intoxication from two families that were initially misdiagnosed as rheumatic disorders. We also performed a literature review about pediatric cases with mercury intoxication to investigate the clinical findings in children, the source of intoxication, and the current treatment preferences. As in our cases, reported patients were previously misdiagnosed as various infectious and/or rheumatic diseases before the diagnosis of mercury intoxication was established. A delay in diagnosis and treatment can cause serious morbidities and even mortality. We report this case series to emphasize the multisystemic presentation of mercury intoxication, and to remind and provide clues for physicians to recognize this rare toxicologic syndrome.The diagnosis of drowning is one of the most difficult in forensic medicine. Forensic diatomology has been proposed to be useful in solving the diagnosis of drowning and considered to be a reliable indicator of the site of drowning. The Yangtze River and Jialing River are the main rivers in the Chongqing area (China), and a large number of corpses are found in the rivers every year. However, the distribution of diatoms in the rivers was not fully studied. In the presented study, a Microwave Digestion-Vacuum Filtration-Scanning Electron Microscopy (MD-VF-SEM) method was performed to acquire the qualitative and quantitative data of diatoms of water samples collected from 10 different sites of the Yangtze River and Jialing River in Chongqing section during different seasons. Our study not only created the diatomological maps of water bodies in Chongqing section of the Yangtze River and Jialing River for the first time but also identified some seasonal and site-specific diatoms that can be taken as markers of particular sites or seasons of drowning. The results of our study may provide forensic scientists helpful reference in solving the drowning cases.