Moonmaynard0712
We present a 44-year-old man with multiple flat papules over a tattoo, diagnosed with acquired verruciform epidermodysplasia (EV). The lesions completely disappeared after 3 sessions of photodynamic therapy (PDT) with topical methyl aminolevulinate. PDT could be considered a treatment of choice in human papillomavirus lesions located over tattoos since it resolves the lesions while preserving the integrity of the design.
The aim of the current study was to assess the effect of different conditioning methods on Lithium di silicate (LDC) employing conventional and contemporary regimes bonded to metallic brackets.
70 discs of LDC were prepared and polished to attain smooth surface. Samples were allocated into seven groups according to ceramic surface conditioning. Group 1 surface treated with Er-YAG laser and saline (S), Group 2 PDT using MBP + S, Group 3 H F + S (control), Group 3 HF (Hydrofluoric acid) + saline, Group 4 HF (Hydrofluoric acid) + ultrasonic bath (UB) + S, Group 5 sand blasting the glass ceramic surface with 120 um Al
O
, Group 6 LDC surface conditioned with SECP(Etch and Prime) and Group 7 ECL(Laser) + S on was irradiated on LDC. Both chemical and mechanical surface treatments of LDC were followed by placement of metallic brackets. Samples were arranged in universal testing machine for shear bond strength (SBS) testing. Bond failure of brackets were assessed using ARI. To assess and compare the mean and stDT for surface treatment of LDC and bonded to metallic bracket is not recommended as it results in decreased bond durability. Use of ECL-S and HF + UB + S has a potential to be used alternatively to HFS for LDC conditioning.
Fluorescence appears clearly in oral biofilm in red tones, showing the presence of microorganisms in regions where there is biofilm accumulation. This study aims to evaluate the applicability and effectiveness of the diagnosis of oral biofilm with the optical fluorescence technique using the EVINCE (Evidenciador Clínico - MMOptics, São Carlos, SP, Brazil) equipment. Furthermore, to compare the efficacy of the optical fluorescence diagnostic method with the traditional method of clinical disclosure of Fuchsin-based dye biofilm and to observe their combined use.
Sixteen children, aged between 7 and 12, were included in this case series, following the Oral Hygiene Index - Simplificated (OHI-S) evaluation. They were evaluated by 3 different professionals. The 1 st evaluator checked the OHI-S observing only with EVINCE. In the second stage, a 2nd evaluator performed the traditional disclosure technique with Fucsina, and finally a 3rd evaluator who observed with EVINCE the teeth previously stained in stage 2, combining the two methods. Descriptive analysis of the variables was performed and comparative tests of repeated measures to evaluate differences between the results of the three evaluation methodologies.
There is no significant difference between the observation made only with EVINCE and with the traditional methodology of plaque disclosure. However, there is a difference when the two techniques are used in the third evaluation moment, showing that the combination could provide better results.
The association of both the conventional method and the use of EVINCE showed a very satisfactory result for the diagnosis of the presence of biofilm.
The association of both the conventional method and the use of EVINCE showed a very satisfactory result for the diagnosis of the presence of biofilm.
The improvement of gene therapy provides hope for the treatment of cancer. However, malignant tumor is a multifactorial disease, which remains difficult to be cured with a single therapy. Our previous study reported that mitochondrial genes glioblastoma-amplified sequence (GBAS) plays a role in the development and treatment of oral squamous cell carcinoma (OSCC). The current study focused on building a mitochondrial-targeting drug co-delivery system for combined photodynamic therapy (PDT) and gene therapy.
5-aminolevulinic acid (ALA) photosensitizer loaded chitosan (CS) nanoparticles were prepared using ionic crosslinking method, and further synthesized with the GBAS gene plasmid DNA (shGBAS) by electrostatic attraction. We detected the effects of PDT using the co-delivery system (CS-ALA-shGBAS) on cell proliferation and mitochondrial injury by MTT and reactive oxygen species (ROS) assays, respectively. Additionally, a oral cancer Xenograft model of nude mice was built to test its inhibitive effect on the cancerous growth in vivo.
A novel nanocomposite, CS-ALA-shGBAS, was found to be spherical structures and had good dispersion, stability and hypotoxicity. Gel retardation assay showed that CS-ALA nanoparticle could synthesize shGBAS at and above Nanoparticle/Plasmid ratios of 1/2. Excitingly, the co-delivery system was suitable for transfected cells and displayed a superior mitochondrially targeted killing effect on OSCC in vitro and in vivo.
Our study provides evidence that the chitosan-based co-delivery system of ALA-induced protoporphyrin IX (PpIX) photosensitizer and GBAS gene may be a novel mode of combined therapy for OSCC.
Our study provides evidence that the chitosan-based co-delivery system of ALA-induced protoporphyrin IX (PpIX) photosensitizer and GBAS gene may be a novel mode of combined therapy for OSCC.The main well recognized action of bisphosphonates (BPs) is their antiresorptive capacity, making them first-line drugs in the treatment of osteoporosis and other metabolic bone diseases. In this review we have compiled other possible actions of BPs, particularly in the areas of immunomodulation, anti-inflammatory capacity and in the prevention of structural joint damage in inflammatory rheumatic diseases. The immunomodulatory capacity of BPs has been focused on the mechanisms involved in the acute-phase response associated with the administration of nitrogen containing BPs (N-BPs), with the stimulus of pro-inflammatory cytokines, through the mevalonate pathway, activation of T-cells and the decrease in the cytotoxic T-lymphocyte antigen-4 (CTLA-4). In relation to their anti-inflammatory capacity, special attention has been given to their effect on preventing structural damage in inflammatory joint diseases and on the differential immune response in bone lesions of the most common and representative inflammatory rheumatic diseases, i.e. rheumatoid arthritis and spondyloarthropathies. The present data indicate that more studies are needed to improve the knowledge on the effect of BPs on inflammatory-mediated diseases and particularly on the prevention and/or treatment of the structural damage in these disorders, since these agents could be a potential useful concomitant therapy.Magnesium (Mg2+), as an essential mineral, supports and sustains the health and activity of the organs of the human body. Despite some clinical evidence on the association of Mg2+ deficiency with muscle regeneration dysfunction and sarcopenia in older-aged individuals, there is no consensus on the action mode and molecular mechanism by which Mg2+ influences aged muscle size and function. Here, we identified the appropriate Mg2+ environment that promotes the myogenic differentiation and myotube hypertrophy in both C2C12 myoblast and primary aged muscle stem cell (MuSC). Through animal experiments, we demonstrated that Mg2+ supplementation in aged mice significantly promotes muscle regeneration and conserves muscle mass and strength. Mechanistically, Mg2+ stimulation activated the mammalian target of rapamycin (mTOR) signalling, inducing the myogenic differentiation and protein synthesis, which consequently offers protections against the age-related decline in muscle regenerative potential and muscle mass. These findings collectively provide a promising therapeutic strategy for MuSC dysfunction and sarcopenia through Mg2+ supplementation in the elderly.
This study aims to analyse the influence of different lining material, thickness and coverage on residual stress of class II molar restoration by the multilayer technique through the three-dimensional (3D) finite element (FE) method. The objective is to reveal the correlations between the base layer configurations and the residual stress distributions of the tooth.
A 3D reconstructed model of an extracted first molar was built through micro-CT images and a class II mesio-occlusal-distal cavity was prepared using computer-aided engineering (CAE) software. A bilayer technique was then applied, and 3D FE analyses were performed under polymerization contraction loading. Glass ionomer composites (Vitrebond and Ionosit) and flowable composite resin (Luxa Flow and Z350 Flowable Restorative) were used as lining materials. 8-OH-DPAT Moreover, several sensitivity analyses with dozens of hypothetical lining materials were conducted to provide definitive results. The thickness of the base layers was set to be distinct (0.5 mm layer technique is an effective way to prolong the service life of resin composites restorations. A thorough evaluation of the sandwich technique through the FE method can provide a better understanding of the stress distributions of the restoration, and reveal its internal mechanisms.
The multilayer technique is an effective way to prolong the service life of resin composites restorations. A thorough evaluation of the sandwich technique through the FE method can provide a better understanding of the stress distributions of the restoration, and reveal its internal mechanisms.
The classification of human peripheral blood cells yields significance in the detection of inflammation, infections and blood cell disorders such as leukemia. Limitations in traditional algorithms for blood cell classification and increased computational processing power have allowed machine learning methods to be utilized for this clinically prevalent task.
In the current work, we present BloodCaps, a capsule based model designed for the accurate multiclassification of a diverse and broad spectrum of blood cells.
Implemented on a large-scale dataset of 8 categories of human peripheral blood cells, the proposed architecture achieved an overall accuracy of 99.3%, outperforming convolutional neural networks such as AlexNet(81.5%), VGG16(97.8%), ResNet-18(95.9%) and InceptionV3(98.4%). Furthermore, we devised three new datasets(low-resolution dataset, small dataset, and low-resolution small dataset) from the original dataset, and tested BloodCaps in comparison with AlexNet, VGG16, ResNet-18, and InceptionV3. To further validate the applicability of our proposed model, we tested BloodCaps on additional public datasets such as the All IDB2, BCCD, and Cell Vision datasets. Compared with the reported results, BloodCaps showed the best performance in all three scenarios.
The proposed method proved superior in octal classification among all three datasets. We believe the proposed method represents a promising tool to improve the diagnostic performance of clinical blood examinations.
The proposed method proved superior in octal classification among all three datasets. We believe the proposed method represents a promising tool to improve the diagnostic performance of clinical blood examinations.
One of the most life-threatening comorbidities in elderly cancer patients is cancer cachexia, which is characterized by the ongoing loss of skeletal muscular strength and mass and is also associated with aging. There is a lack of recommendations for optimal resistance training (RT) for those patients. The purpose of this study was to systematically review and quantify the effects of RT on muscular strength and hypertrophy in elderly cancer patients.
Five electronic databases were searched (until January 2020) for studies that met the following criteria (i) cancer patients aged ≥60 years; (ii) structured and supervised RT intervention for ≥6 weeks; and (iii) measured muscular strength and/or hypertrophy.
Thirteen studies (717 participants, average age = 66 years) met the inclusion criteria. RT significantly increased muscular strength (mean effect size = 0.87, 95% confidence interval (95%CI) 0.43-1.32, p < 0.001) and did not significantly induce muscle hypertrophy (mean effect size = 0.09, 95%CI -0.14 to 0.
