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A data structure is proposed that can store forensic data obtained by experts from different disciplines and acquired using different instruments. This data structure, called TraceBase, is congruent with the forensic examination in the laboratory. We describe the design as well as its planned introduction in casework. The back-end of TraceBase is based on PostgreSQL and can be accessed by front-end applications such as the open-source LibreOffice office suite. The back-end regulates the flexible and robust storage of data, as well as the relation between items, samples, and analyses. The front-end applications allow the user to enter or retrieve data in an easy fashion, while the modular structure ensures that different aspects, such as the data entry, the processing and reporting of entered data, can be optimised individually. Additional analyses can be introduced and linked to items or samples already present. The database is designed such that data from several sources, different forensic disciplines and data acquired by different analytical techniques can be entered. When data needs to be retrieved for further analysis, a subcollection can be filtered for use in a specific situation.Marijuana, dried and ground Cannabis, is the most consumed illicit drug in the world. Many undesirable and risky effects to human health are caused by its use. The medicinal use or legal recreational use of Cannabis has also been rising in many countries. These facts make traceability methodologies increasingly important whether for forensic use, such as drug trafficking eradication, or for quality control purposes of legal medicinal Cannabis. Consequently, the objective of this study was to analyze Cannabis by means of thermogravimetric analysis (TGA) in order to assess the capability of this technique to trace the geographical origin of Cannabis cultivated in Colorado, United States of America. TGA appears to be sensitive enough to detect the degradation/decarboxylation of cannabinoids and terpenes, at least to some extent; also, the degradation of cellulose, hemicellulose and lignin was indicated. Overall, the temperature ranges we analyzed using linear discriminant analysis showed high accuracies, with the 200 to 300 °C and 600 to 700 °C ranges achieving 100% accuracy."Shedder status" or "shedder type" are commonly used terms that categorise an individual based on their ability to deposit "touch" DNA via direct contact with a surface. However, it is not yet clear how best to categorise an individual into a shedder class, or how to allocate a shedder score on a sliding scale. This study considers categorisation of participants into discrete shedder categories based on DNA quantity and profile quality data, the maintenance of their shedder status over an extended period, and explores whether different methods of deposition or collection directly from hands or other body areas are interchangeable and/or more appropriate means of determining an individual's shedder status. The shedder categorisation of participants was possible from their handprints and remained unchanged over three years. Washing hands had limited impact and shedder categorisation was not readily possible from samples collected directly from hands, other body areas or gloves after wearing gloves for a set duration. Use of consecutive deposits may assist in establishing a participant's shedder status. As shedder categorisation may be of relevance during activity level assessments further efforts towards the ability to do so are necessary.Identifying vaginal secretions attaching or adhering to a suspect's belongings would be beneficial for reconstructing the events that have taken place during a sexual assault. The present study describes a novel approach to identify vaginal secretions by fragment analysis using capillary electrophoresis, based on the mobility differences of PCR amplicons from bisulfite-treated DNA depending on methylation status. We targeted three genome regions including each of three vaginal secretion-specific methylated CpG sites reported previously cg25416153, cg09765089, and cg14991487. In all three genome regions, the amplicon peaks for methylated genomic DNA (gDNA) sequences were only detected in vaginal samples, whereas samples of other body fluids (blood, saliva, semen, and deposit on skin surface) only showed amplicon peaks for unmethylated gDNA sequences. In vaginal secretions, the methylation ratio of each of the three targeted regions between samples was variable, while the ratios at the three regions in each sam potential to analyze multiple marker regions simultaneously, and may provide a new multiplex assay to identify various body fluids.Latent fingermarks are important trace evidence found in crime scenes mainly used for identification purposes. Once deposited, the composition of samples changes over time influencing the efficacy of latent fingermark development methods. In this sense, the aims of this work were to characterize the fatty acid (FA) profile of sebaceous latent fingermarks by GC-FID and to preliminarily evaluate the development efficiency of enhancement methods (powder dusting, iodine fuming and silver nitrate method) in a 30-day period of aging under controlled parameters of temperature, photoperiod and humidity. Results showed that myristic (7.51 ± 0.76% - 13.39 ± 1.26%), palmitic (35.91 ± 1.07% - 40.81 ± 2.52%), stearic (6.67 ± 0.36% - 9.13 ± 0.36%) and oleic (18.08 ± 0.25% - 20.93 ± 0.26%) acid varied significantly (p less then 0.05) over the 30-day period of analysis. Androgen Receptor Antagonist Regarding development efficiency, fluorescent orange powder and the silver nitrate method also increased their efficacy to develop latent fingermarks over time while the iodine fuming method decreased its efficiency. Silver black powder had constant efficacy in the tested period. Changes in the constitution of sebaceous marks possibly influenced the development efficiency of enhancement techniques. This knowledge is important to better understand the kinetic of aging and its influence on the development method.There is a body of published research that has evaluated the contribution of forensic science to the criminal justice system, but many disciplines of forensic science remain unexplored in this regard. The aim of this study was to examine the contribution that forensic fire examination services provide to criminal investigations and court processes in arson cases. Forensic fire examination services differ in a number of ways to the disciplines covered in previous research on the impact of forensic evidence on justice outcomes. Forensic fire examinations involve a combination of scene examination and laboratory analyses, and the results can provide critical evidence of whether an incident that has occurred is a criminal offence (i.e. whether a fire has occurred as the result of an act of arson). Forensic fire examination is also a discipline that has faced challenges and undergone development in recent decades regarding its scientific basis and the issue of contextual bias. In this study, data were collated for 273 structural fires that were examined by the forensic fire services in Victoria, Australia.