Weinreichskaarup5092

t with suspected LE should raise suspicion of other diagnoses. Identification of a bone spur is of very limited clinical value given the high prevalence in the general population. The important outcomes of pain, disability, PRTEE, and disease duration did not correlate with any of the investigated US techniques.Adipose tissue inflammation, as defined by macrophage accumulation, is proposed to cause insulin resistance and systemic inflammation. Because the strength of this relationship for humans is unclear, we tested whether adipose tissue macrophage (ATM) burden is correlated with these health indicators. Using immunohistochemistry we measured abdominal subcutaneous CD68+ (total ATM) , CD14+ (pro-inflammatory/M1), and CD206+ (anti-inflammatory/M2) ATM in 97 volunteers BMI 20 - 38 kg/m2, in addition to body composition, adipocyte size, HOMA-IR, ADIPOpalmitate-IR, plasma lipids, TNF and IL-6 concentrations. There were several significant univariate correlations between metabolic parameters/IL-6 and ATM/100 adipocytes, but not ATM/gram tissue; adipocyte size was a confounding variable. We used matching strategies and multivariate regression analyses to investigate the relationships between ATM and inflammatory/metabolic parameters independent of adipocyte size. Matching approaches revealed that the groups discordant for CD206, but concordant for adipocyte size, had significantly different fasting insulin and IL-6 concentrations. However, groups discordant for adipocyte size, but concordant for ATM differed in that visceral fat, plasma triglyceride, glucose and TNF concentrations were greater in those with large adipocytes. Multivariate regression analysis indicated that indices of insulin resistance and fasting triglycerides were predicted by body composition; the predictive value of ATM/100 adipocytes or per gram tissue was variable between males and females. We conclude that the relationship between ATM burden and metabolic/inflammatory variables is confounded by adipocyte size/body composition and that ATM do not predict insulin resistance, systemic inflammation or dyslipidemia. ATM may primarily play a role in tissue remodeling rather than metabolic pathology.Objective Obesity induced by a high fat diet is associated with chronic up-regulation of inflammatory cytokines which stimulate osteoclast activity and bone resorption. selleck chemicals llc However, the role of high-fat diet on bone-implant connectivity has not been studied in detail. In this study, we investigated whether a high-fat diet (HFD) affects bone implant connection (BIC) in periimplant bone. Methods Twenty female Sprague Dawley rats were divided in two groups 1) Control rats were fed with normal chow and titanium implants were integrated into tibial bones at the end of 3rd month and no treatment was applied 2) HFD group; rats were fed a high-fat diet (42 % of calories as fat), then the titanium implants were integrated into tibial bones at the end 3rd month. Following surgical integration of the implants, the rats were fed with control and HFD diets for 3 months. After the 6 months experimental period all rats were sacrificed and the implants and surrounded bone tissues were collected and the BIC was assessed histomorphometrically after the non-decalcifiing histological methods. Bone implant connection was detected with the ratio of the implant surface directly connected with the peri-implant bone tissues to the total implant surface length. Results Histologic analysis showed that HFD was not impaired BIC (p>0.05). Conclusion In conclusion, within the limitation of this research, HFD did not effect the BIC rat tibias (Tab. 2, Fig. 2, Ref. 26). Text in PDF www.elis.sk.Objectives The objectives of this study were to determine the prognostic value of expression levels of selected biomarkers and their statistical analysis in relation to survival and standard histopathologic examination and other clinicopathologic variables in non-muscle invasive bladder cancer (NMIBC). Background Worldwide, bladder cancer is a frequent malignant disease with rising incidence. Characteristic invasiveness and high recurrence rates call for more diagnostic methods to obtain more accurate information. Prognosis is affected by a significant interpersonal variability of the disease. For this reason, constant search for alternative and better diagnostic methods is essential. Methods We analysed cancer tissue from patients with Ta and T1 bladder cancer. E-cadherin and Ki-67 expression levels were analysed using immunohistochemical staining. The expression levels quantified to a percentual amount were statistically analysed in relation to survival and their frequency distribution in the study group. Results E-cadherin and Ki-67 expression levels show high association with tumor stage and grade (p less then 0.001), in contrast, the association with recurrence has proven insignificant. Patients with non-aberrant biomarker expression levels have much higher survival rates than the cases with aberrant expression. Conclusion Low expression levels of Ki-67 and high expression levels of E-cadherin positively affect survival of patients, whereas aberrant expressions pose poorer prognosis (Tab. 2, Fig. 2, Ref. 33).Objectives The ependymal lining of the human brain ventricular system displays distinct structural differences and functional heterogeneity among individual ependymal cells (ECs). To date, multi-ciliated ECs (E1 cells), bi-ciliated ECs (E2 cells), uni-ciliated ECs (E3 cells), ECs without cilia, and ECs with cytoplasmic protrusions have been described in human brain ventricles. Method Using scanning electron microscopy (SEM), we evaluated ependymal samples from 6 defined regions of the third ventricle from 9 human brains. These regions were strictly defined according to the periventricular structures they neighbour with. Results We observed different structures on the apical surface of the ECs. Various ECs differed from each other by the presence of microvilli, secretory bodies, and a variable number of cilia, which led us to divide the ECs into several exactly specified types according to their apical morphology. Conclusion We found all types of ECs in every examined region with a predominance of particular types of apical surface of ECs in the individual areas (Tab. link2 4, Fig. 7, Ref. 22).This study aimed to evaluate the outcomes of carotid endarterectomies (CEA) performed using transcranial cerebral oximetry as cerebral monitoringMETHODS This single-center study included patients treated surgically for significant stenosis of the internal carotid artery (ICA) from January 2012 to December 2017RESULTS Of the 654 patients, 267 were asymptomatic, and 387 were symptomatic. link3 Eversion CEA was performed in 64.8 %, and patched conventional CEA in 35.2 % of all patients. Totally 11.4 % of all patients had a shunt inserted; all patients with the inserted shunt had the conventional pathed CEA. Severe postoperative complications occurred in 1.5 % of asymptomatic patients and 2.6 % of symptomatic patients. The stroke and death ratio in the shunted group was 2.7 %, and in the non-shunted group, 2.1 % (p = 0.7). We found no significant difference in severe postoperative complications between the shunted and non-shunted group. Further, we found the male gender (p = 0.005), coronary artery disease (p = 0.01), and ongoing smoking (p = 0.003) to be significantly associated with neurological symptoms of the ICA stenosis. We also confirmed current tobacco smoking to be significantly associated with the occurrence of postoperative stroke and death (p = 0.005)CONCLUSION We found transcranial cerebral oximetry to be reliable in the determination of shunt insertion (Tab. 6, Ref. 26).Objectives Many studies use stimulated saliva for the assessment of cortisol. However, it is not yet clear how stimulation affects the flow of specific markers. The aim was to assess whether stimulation of salivation affects the physiological flow of cortisol during a stressing day as compared to an ordinary day. The second aim was to show how the normalising factor affects the outcome of the study. Methods Stimulated saliva was taken from 42 children at 800 a.m. and 1200 a.m. on two separate days one month apart. During the first day, the children were exposed to stress situation, while the second day was considered a control day. The concentration of cortisol was analysed using ELISA. Results The highest level of cortisol was observed in the morning of the stress day (p 0.99). Conclusion Based on our results, the examination of the cortisol diurnal rhythm is not reliable in stimulated saliva. Moreover, the effect of saliva stimulation has to be taken into account for every marker individually (Fig. 2, Ref. 22).Introduction Myelodysplastic syndromes (MDS) include various hematologic abnormalities characterized by chronic cytopenia due to disruption in cellular differentiation. This study aims to evaluate the prognostic value of PLR in patients with MDS. Material and methods Clinical-laboratory findings and the results of bone marrow biopsies of MDS patients before treatment were recorded. p value of less then 0.05 was considered statistically significant. SPSS version 20.0 was used for statistical analysis. Results The study included 62 patients with median follow-up time of 62.8±4.5 months and median age of 68.5 years. In 13 patients, acute leukemia was transformed. In these subjects, a PLR cut-off level of 46 was established for mortality (p=0.015). We found a significant relationship between PLR and multilineage series with the presence of dysplasia (p=0.017). The survival analysis showed a decreased survival in cases with dysplasia in two and/or more series, transformation into acute leukemia, and thrombocytopenia. Conclusion Our study demonstrated that there was a relationship between PLR and MDS with multilineage dysplasia (mld-MDS). PLR is investigated as an inflammatory finding in various hematologic malignancies. Further studies investigating the value of PLR in MDS are needed to determine whether PLR may be a marker of bone marrow dysplasia grading (Tab. 2, Fig. 4, Ref. 32).Aim This study aimed to assess the psychometric characteristics of the Slovak translation of the version A of Wijma Delivery Expectancy/Experience Questionnaire (SW-DEQ) among healthy Slovak pregnant women. Method Exploratory factor analysis (EFA) of the SW-DEQ was performed. Several coefficients of internal consistency were employed for the whole scale as well as for separate factors. Concurrent/convergent validity was assessed by correlation analysis of the overall SW-DEQ score with State-Trait Anxiety Inventory, Eysenck Personality Inventory - subscales Neuroticism and Extraversion, as well as with adjusted versions of Beck Depression Inventory and Internal-External Locus of Control Scale. EFA yielded seven factors 'lack of composure', 'negative appraisal', 'lack of self-efficacy', 'lack of positive anticipation', 'fear and hopelessness', 'loneliness', and 'concern for the child'. Results The Cronbach's α of the whole scale was .93, while for the separate factors, it ranged between .68 and .87. The sum score of SW-DEQ correlated weakest with extraversion (in nulliparous women) and locus of control (in multiparous women), and strongest with trait anxiety (in both groups). Conclusions The Slovak version could be considered a valid and reliable measure of fear of childbirth among pregnant Slovak women. However, the dimensional structure of the measure warrants further confirmation (Tab. 7, Ref. 33).