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Pseudomonas putida is potentially used in the bioremediation of heavy metals (HMs). Its response to different HMs in different environments is still not fully understood. This study investigated resistance against 12 kinds of metals by P. putida CZ1 planktonic cells and its biofilm in LB and mineral medium (MM). P. putida CZ1 biofilms have high resistance and accumulation capacity for Cu2+, Zn2+, Pb2+, Fe3+, Mn2+, Al3+ and Ni2+, but less resistance to Co2+, Cd2+, Cr2O72-, Ag+ and Hg2+. Biofilms were 2-8 times more resistant to Cu2+ and Zn2+ than planktonic cells. There was a strong correlation between the P content and the accumulation of Cu2+, Zn2+, Fe3+, Mn2+, Pb2+, Ni2+and Al3+ respectively. Confocal laser scanning microscopy (CLSM) combined with live/dead staining study found that cells in the biofilms can keep viable after 36 h under MIC of Cu2+ or Zn2+ both in LB and MM. When the metal concentration increased, cells can be killed gradually. For Cu2+, Zn2+, Fe3+, Mn2+, Pb2+ and Ni2+, higher resistance was found in MM (2-4 times higher) than in LB and higher accumulation of these metals were also found in MM. P. putida CZ1 biofilm cultured in MM with citric acid as carbon source had stronger resistance and accumulation ability to Cu2+, Zn2+, Pb2+, Fe3+, Mn2+, and Ni2+. This suggested that P. putida CZ1 had greater remediation potential for these metals in organic acid rich environments.For the first time, spent Zn-MnO2 primary batteries are recycled to directly build rechargeable Zn-MnO2 batteries with a mixed solution of sulfuric acid and hydrogen peroxide as the leachate, which aimed to the efficient recovery of spent Zn-MnO2 primary batteries and the realization of high-powered rechargeable Zn-MnO2 batteries. After simple purification, the leached liquid is directly used as the working solution to prepare an electrolytic rechargeable Zn-MnO2 battery. The experimental results show that the performance of the recycling solution of the neutral Zn-MnO2 primary battery was better than that of the alkaline Zn-MnO2 primary battery, and both performed better than the solution prepared with chemically pure reagents. After optimizing the pH of the working solution and charging current, the obtained rechargeable Zn-MnO2 battery can provide an energy efficiency of 72.33 % ± 0.55, a coulombic efficiency of 90.17 % ± 0.71, and excellent cycle stability. These experimental results show that spent Zn-MnO2 primary batteries can be successfully recycled to prepare rechargeable Zn-MnO2 batteries, demonstrating very good application potential.Terrestrial oil spills are a major threat to environmental and human well-being. Rapid, accurate, and remote spatial assessment of oil contamination is critical to implementing countermeasures that prevent potentially lasting ecological damage and irreversible harm to local communities. Satellite remote sensing has been used to support such assessments in inaccessible regions, although mapping small terrestrial oil spills is challenging - partly due to the pixel size of remote sensing systems, but also due to the distinguishability of small oil spill areas from other land cover types. We assessed the usability of freely available Sentinel satellite images to map terrestrial oil spills with machine learning algorithms. Using two test sites in South Sudan, we demonstrated that information from the Sentinel-1 and -2 instruments can be used to map oil spills with more than 90 % classification accuracy. Classification accuracy was significantly increased (>95 %) with the addition of multi-temporal information and spatial predictor variables that quantify proximity to oil production infrastructure such as pipelines and oil pads. The mapping of terrestrial oil spills with freely available Sentinel satellite images may thus represent an accurate and efficient means for the regular monitoring of oil-impacted areas.This study investigated effects of different thermal processes on characteristics of activated carbon to produce efficient biosorbents or supercapacitors using biomass resources. Pyrolysis char and hydrochar obtained from woody biomass were used as precursors for activated carbon under different atmospheric conditions (N2 and air). In order to provide functional groups on the carbon surface, activated carbon under N2 condition was subsequently acidified by HNO3 and the other was simultaneously acidified under air condition. Additionally, potential for application as Pb2+ adsorbent and supercapacitor was evaluated. Thermochemical behaviors such as bonding cleavage and dehydration during activation processes were observed by TG and Py-GCMS analysis. Elemental analysis, FT-IR, Raman spectroscopy, and XPS analysis were carried out to confirm changes in structures of each carbon products. New plausible reaction mechanism for this observation was suggested with respect to the formation of a key intermediate in the presence of excess air. As for performance in applications, air activated carbon using hydrochar exhibited high versatility to function as both Pb2+ adsorbent (~41.1 mg/g) and energy storage material (~185.9 F/g) with high specific surface area, mesopore ratio, surface functional groups.Improving water use efficiency from a recycling perspective is claimed to be a better way to ensure prosperity in a water-stressed world. Although many studies have focused on the efficiency of integrated water use system, such as a water use and wastewater treatment (WUWT) system, few studies have observed the dynamic change involving a two-stage recycling structure. This research thus proposes a dynamic two-stage recycling model within the directional distance function (DDF) by taking reused water in the WT stage as a recycled product for WU stage's input in the next period. This paper reveals the dynamic evolution and spatial migration path of this efficiency in China. Results are as follows. (1) The average overall efficiency of the urban WUWT system for 30 provinces during 2011-2018 was 0.78. The central region's lower WUWT efficiency was caused by the expanding provincial differences in WU efficiency. (2) The urban WU sub-system performed poorer than that of the urban WT sub-system due to underutilizing reused water, such as in Jiangxi, Guangxi, Yunnan, and Jilin. (3) Narrowing the spatial imbalance of economic development and water use between the eastern and western regions is of great importance for promoting China's spatial imbalance of urban WUWT efficiency.Despite major recent advances in socio-hydrology and hydroeconomics research, interdisciplinary methods and models for water policy assessment remain largely concealed to the academic arena. Most river basin authorities still base decision-making on inputs from hydrologic Decision Support Systems (DSS), and have limited information on the economic costs that water policies may impose on the economy. This paper presents a time-variant hierarchical framework that connects a hydrologic module and an economic module by means of two-way feedback protocols. The hydrologic module is designed to fit the AQUATOOL DSS, the hydrologic model used by Spanish river basin authorities to inform decision-making at a basin scale; while the economic module is populated with a Positive Multi-Attribute Mathematical Programming (PMAMP) model that represents the behavior and adaptive responses of irrigators. The proposed hierarchical framework is used to assess the economic repercussions of strengthening irrigation quotas so to achieve minimum environmental flows in the Douro River Basin (Spain) under climate change. Results show that reductions in agricultural water allocations to meet environmental flow requirements create nonlinear incremental profit and employment losses in irrigated agriculture that are on average low to moderate (between -4% and -12.9 % for profit, and between -4.6 % and -12 % for employment, depending on the scenario). During extreme droughts, the abrupt reductions in water availability and agricultural allocations can test farming systems past the breaking point and lead to catastrophic profit and employment losses (>80 %).In recent years, organophosphate ester flame retardants (OPFRs), which have been regarded as alternatives for brominated flame retardants (BFRs), have become widely used in building materials, textiles, and electric equipment. Elucidating the relationship between OPFRs and tumors holds great significance for the treatment and prevention of diseases. In this work, we found a new method for predicting the correlation between the interactive genes of OPFRs and tumors. Transcriptome profiles and OPFR information were obtained from The Cancer Genome Atlas and the Genotype-Tissue Expression, Comparative Toxicogenomics, and PharmMapper databases. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis showed that interactive genes were mainly enriched in prostate cancer, steroid metabolic process, and steroid hormone regulation. Furthermore, protein-protein interaction network analysis revealed 33 biological hub genes. The operating characteristic curves and survival analysis showed the role of key genes in predicting the prognosis of prostate cancer. Gene target prediction and gene set variation analysis proved that OPFRs and their metabolites exert potential effects on prostate cancer. Colony formation assay showed that the cells with AR, mTOR and DDIT3 knockdown could remarkably mitigate the cell proliferation ability in both PC-3 and LNCap cells. Transwell assay demonstrated that the silencing of AR, mTOR and DDIT3 could significantly inhibit the cell invasion capacity of prostate cells. Triphenyl phosphate (TPP) significantly increase the cell proliferation ability and promote cell invasion capacity. AR, mTOR and DDIT3 in the PC-3 and LNCap cells were significantly upregulated with 10-6 M TPP treated.Food dyes, or color additives, are often added into foods, cosmetics and beverages during processing to improve the sensory attributes of the final products. However, the toxicity of tartrazine (TZ), one of the most common azo-dyes, is still unclear, and needs to be ascertained by further study. this website Hence, in the present study, we aimed to evaluate the effects of TZ consumption on health by using a teleost, crucian carp (Carassius auratus) as the experimental fish. TZ consumption (1.4, 5.5 and 10 mg/kg bwt/day) could cause severe histopathological and cellular alterations in intestine and liver. The height of intestinal villus, thickness of intestinal muscle, and microvilli density were also affected. With the increasing of TZ concentrations, the activities of antioxidant enzymes (CAT, SOD and GSH-Px), exhibited a decreasing trend, while the contents of MDA elevated. Upregulations of pro-inflammatory cytokines (il6 and tnfα), anti-inflammatory cytokines (il8, and il10) and other immune related genes (complement component 3 (c3), lysozymes (lyz), β-defensin 3 (defb3)), were observed after TZ uptake. In addition, TZ consumption also affected the community structure of the microbiota in the intestine of crucian carp. The amount of some probiotic bacteria (Roseomonas, Rhodococcus and Bacillus) and the bacteria (Bacteroides and Clostridium), producing short chain fatty acids, were significantly reduced, and some pathogenetic microorganisms (e.g. Bdellovibrio and Shewanella) were significantly increased after TZ uptake. In summary, the data in the present study indicate that TZ consumption, even at a low concentration, may lead to adverse effects on fish health. Therefore, in aquaculture, it is necessary to be informed about the hazardous effects of TZ, and more attentions should be focused on using natural substitutes.