Meyersstensgaard0384

Due to the steric effects, significantly lower Pt concentrations were accumulated in the spheroids treated with TR-CDDP (2.2 times lower than in CDDP-treated spheroids, normalized to the spheroid volume), while the Pt was mostly distributed in the areas of the outer rim. Finally, imaging with confocal fluorescence microscopy, which is commonly used in oncology research, was compared with that by LA-ICP-MS. The results of the two complementary techniques demonstrated good agreement in terms of the spatial distribution of the TR-CDDP, while the intensity of the fluorescence matched well with the concentrations of Pt determined with LA-ICP-MS.The solid contact ion-selective electrodes (SC-ISEs) have been extensively studied in the field of ion sensing as they offer the possibility of miniaturization, are relatively inexpensive in comparison to other analytical techniques and allow straightforward and routine analyses of ions in a number of clinical, environmental and industrial process samples. In recent years, significant interest has grown in the development of SC-ISEs with well-defined interfacialpotentials at the membrane, solid contact, and substrate electrode interfaces. This has resulted in interesting SC-ISEs exhibiting high electrode-to-electrode potential reproducibility, for those made in a single batch of electrodes, some approaching or exceeding those observed in liquid-contact ISEs. The advancement in the potential reproducibility of SC-ISEs has been partially achieved by scrutinizing insufficiently reproducible fabrication methods of SC-ISEs, or by introducing novel control measures or modifiers to components of the ISEs. This paper provides an overview of the methods as well as the challenges in establishing and maintaining reproducible potentials during the fabrication and use of novel SC-ISEs. The rules outlined in the works reviewed may form the basis of further development of cost-effective, user-friendly, limited calibration or calibration-free potentiometric SC-ISEs to achieve reliable ion analyses here and now.Labeling with redox reporter is often required in developing electrochemical bioassay for most proteins or nucleic acid biomarkers. Herein, a label-free ratiometric immunosensing platform is firstly developed by integrating the antibody-conjugated nanochannels with a smart modified electrode. The electrode modifier is the composite of C60, tetraoctylammonium bromide (TOA+) and Prussian blue (PB). Cyclic voltammograms of the ultimate C60-TOA+/PB modified electrode exhibited two pairs of peaks at 0.15 V and -0.13 V, ascribing to the redox of PB and C60, respectively. With the addition of K3[Fe(CN)6] in the electrolyte solution, the peaks of PB decreased due to the adsorption of [Fe(CN)6]3- while the peaks of C60 increased because of the formation of the ternary complex (TC) C60-TOA+-[Fe(CN)6]3-. As a result, the peak current ratio IPB/ITC decreased gradually with the increment of the concentration of [Fe(CN)6]3-. For the nanochannels-based immunosensing platform, the steric hindrance of the bioconjugated nanochannels varied with the loading amount of the target CA125, and thus [Fe(CN)6]3- passing through the channels was quantitatively affected. And the higher CA125 level was, the less [Fe(CN)6]3- concentration was. And thus, the ratio IPB/ITC monitored at the C60-TOA+/PB modified electrode increased with the increase of the concentration of CA125. The ratiometric immunoassay featured a linear calibration range from 1.0 U mL-1 to 100 U mL-1 with a low detection limit of 0.86 U mL-1. click here In addition, the ratiometric immunosensing platform demonstrated good specificity and stability as well as acceptable accuracy in overcoming the effect of electrode passivation which was an inherent problem of electroanalysis.Nanoparticle plasmon scattering can provide real-time imaging information on the formation process of noble metal-based nanomaterials. Due to the synergistic effect of the interface between metal and oxide supporting pores, metal nanoparticles (NPs), especially Au NPs, generally exhibit higher catalytic activity on oxide carriers than single-component NPs. Here, we use the dark field scattering microscope to in situ monitor the growth of Au on Cu2O surface by oxidation-reduction reactions and the nanostructures could be precisely controlled via the scattering signal. The prepared Cu2O/Au nanocomposite has a higher electrocatalytic activity toward Glucose. When being used as a potential biosensor for nonenzyme glucose detection, excellent performance, such as high sensitivity with a detection limit of 4 μM, high selectivity and outstanding stability, was obtained. The scattering imaging strategy is a convenient and universal approach in controllable synthesis of plasmonic heterostructures, and leads to the improvement of electrocatalysts in biosensing.In this work, a novel amino functionalized Cu(II) ion-imprinted organic-inorganic hybrid monolithic column (Cu(II)-IIHMC) was prepared via one-pot co-condensation and the combination of sol-gel and ion-imprinting techniques in a fused capillary. The Cu(II)-IIHMC was used as solid phase microextraction (SPME) matrix followed by inductively coupled plasma-mass spectrometry (ICP-MS) for the analysis of trace Cu(II). The prepared Cu(II)-IIHMC has good mechanical strength, stable imprinting sites and homogeneous structure of network skeleton with large flow-through pores by optimizing the synthesis process. Under the optimized conditions, the Cu(II)-IIHMC can selectively adsorb Cu(II) with the adsorption capacity of 3.13 mg g-1. With enrichment factor of 10-fold, the calibration curve was established in the range of 0.05-50 μg L-1 with r2 of 0.9992 and the detection limit was 0.008 μg L-1 for Cu(II). Compared with the non-imprinted hybrid monolithic column (Cu(II)-NIHMC), the Cu(II)-IIHMC possesses better selectivity, anti-interference ability and adsorption capacity. The Cu(II)-IIHMC can specifically capture the target ion in the presence of competitive ions, with the selectivity coefficients exceeding 39.4. The protocol was validated by analyzing Certified Reference Materials of standard sediment, soil and iron ore, and the results were in good agreement with certified values. Moreover, the proposed in-tube SPME procedure can not only preconcentrate trace Cu(II), but also effectively reduce the matrix effect and powerfully eliminate the interference from the main metals in real samples. Therefore, the developed SPME-ICP-MS method with facile preparation, specific selectivity, high sensitivity and efficient analysis, was applied in the determination of trace Cu(II) in environmental and mineral samples with the recoveries of 89.8-111.8% in all spiked samples.In this study, a fast and simple method for highly sensitive detection of Cd and Pb elements based on atmospheric pressure glow discharge atomic emission spectrometry (APGD-AES) coupling with tungsten coil electrothermal vaporization (ETV) was proposed. A small amount of sample (10 μL) was dropped onto the tungsten coil, followed by drying, pyrolysis and vaporization procedures, and then the vaporized analyte was transported to APGD for excitation. The whole procedure took approximately 3 min. Multi-step heating of the ETV unit can separate matrices and solvents from the analyte, providing an advantage in detecting samples with complex matrix. Under the optimal experimental conditions, limits of detection of 0.4 μg L-1 (4 pg) for cadmium and 1.2 μg L-1 (12 pg) for lead were obtained, with relative standard deviations of 20 μg L-1 Cd and 100 μg L-1 Pb both being less then 5%. The accuracy of the ETV-APGD-AES system was verified by the determination of heavy metals in whole blood standard sample (GBW(E)090,251) and the practicability of the ETV-APGD-AES system were demonstrated by the determination of heavy metals in human whole blood. The results obtained by this instrument agree well with the standard values and those obtained by ICP-MS.

Previous studies have suggested acute effects of ambient fine particulate matter (PM

) air pollution on respiratory health among children, but evidence for PM

constituents and respiratory health were still limited.

To investigate associations of short-term exposure to PM

and its constituents with airway inflammation, lung function, and airway microbiota in children.

We conducted a longitudinal panel study with 3 repeated health measurements among 62 children in Shanghai, China from November 2018 to June 2019. Respiratory health was measured by fractional exhaled nitric oxide (FeNO), saliva tumor necrosis factor-α (TNF-α), lung function (forced vital capacity and forced exhaled volume in 1s), and microbiota diversity in buccal mucosa samples. Based on the linear mixed-effect models, we applied the single-constituent models and the constituent-PM

adjustment models to examine the associations between PM

constituents and health outcomes.

Short-term exposure to PM

was associated with higher TNF-α, FeNO levels and reduced lung function. Among all constituents, organic carbon, elemental carbon, NO



and NH



had the consistent and strongest associations with airway inflammation biomarkers and lung function parameters, followed by metallic elements. We also found short-term PM

exposure was associated with decreased diversity in buccal mucosa bacterial community and two bacterial phyla, Fusobacteria and Proteobacteria, were identified as differential microbes with PM

exposure.

Short-term exposure to PM

may impair children's respiratory health represented by higher airway inflammation, lower lung function and altered buccal mucosa microbial colonization. Organic carbon, elemental carbon, NO



and NH



may dominate these effects.

Short-term exposure to PM2.5 may impair children's respiratory health represented by higher airway inflammation, lower lung function and altered buccal mucosa microbial colonization. Organic carbon, elemental carbon, NO3- and NH4+ may dominate these effects.Tumor predisposition syndromes represent a heterogeneous group of multiorgan disorders, with many having substantial central nervous system involvement. This article highlights the common and uncommon manifestations of these syndromic disorders, the underlying genetic pathways, and the imaging findings. Radiologists must be aware of the diagnostic criteria, optimal imaging techniques (both for diagnosis and surveillance), as well as the innumerable imaging manifestations of these syndromes. Multidisciplinary approach and teamwork are essential in managing these patients, with imaging having a central role as more of these patients get diagnosed earlier and survive longer.Nonneoplastic entities may closely resemble the imaging findings of primary or metastatic intracranial neoplasia, posing diagnostic challenges for the referring provider and radiologist. Prospective identification of brain tumor mimics is an opportunity for the radiologist to add value to patient care by decreasing time to diagnosis and avoiding unnecessary surgical procedures and medical therapies, but requires familiarity with mimic entities and a high degree of suspicion on the part of the interpreting radiologist. This article provides a framework for the radiologist to identify "brain tumor mimics," highlighting imaging and laboratory pearls and pitfalls, and illustrating unique and frequently encountered lesions.