Weavermosegaard8239

Dendritic Cells (DCs), derived from haematopoietic stem cells, are critical to the dynamic and balanced functioning of the intact immune system and are of great interest as vehicles of immunotherapy. Genetically modified mouse models have proved powerful tools to map DC development and function in vivo but human studies have previously relied heavily on in vitro systems. Human dendritic cell immunodeficiency, resulting from single gene mutations, offers new opportunities to dissect the role of human DCs in vivo, determine the genetic requirements for their development and map their haematopoietic differentiation pathways. This review will summarise the clinical phenotypes of mutations in GATA2, IRF8 and IKZF1 genes which result in global or subset specific dendritic cell deficiencies, discuss the functional consequences of these cytopenias and how these syndromes have informed our knowledge of DC differentiation and human haematopoiesis. Nitrogenous wastewater is difficult to treat using conventional microorganisms in high salinity and acidic/alkaline environments. Two halotolerant bacteria, heterotrophic nitrifying Stenotrophomonas sp. MSNA-1 and aerobic denitrifying Pseudomonas sp. MSD4, were isolated, and the amplification of functional genes provided the evidences of nitrogen removal performance. The results regarding salinity and pH resistance showed that strain MSNA-1 is robust at salinities of 0-15% and pH of 3-10. It can remove 51.2% of NH4+-N (180 mg/L) at salinity of 10% (pH 7) and 49.2% of NH4+-N under pH 4 (salinity 3%). For strain MSD4, it is robust at salinities of 0-10% and pH of 5-11. It can remove 62.4% of TN (100 mg/L) at salinity of 7% (pH 7) and 72.2% of TN under pH 9 (salinity 3%). Their excellent salinity and pH resistances make them promising candidates for treating nitrogenous wastewaters under extreme conditions with low operational cost. In this work, accelerated start-up of biological hydrogen production system fed with glucose and molasses at 55 °C by regulating pH and COD concentration was investigated in two groups. Then three reactors in each group were compared controlling pH, controlling pH with COD, and controlling the COD. The reactors in group A presented best hydrogen yield of 1.84 mol H2/mol glucose·day and worked stably at the 8th day. The highest hydrogen yield in group B was 2.13 mol H2/mol molasses·day and steadily at the 11th day. It proved that controlling two key parameters of the inflow pH (8.0) and substrate concentration (4000 mg COD/L) could realize fast start-up of hydrogen production reactor. This study demonstrated that Thermoanaerobacterium sp. strain RBIITD could produce hydrogen and provide a new avenue for biological hydrogen production by dark fermentation using cheap substrate towards a more sustainable and feasible technology. The aim of this work was to study the potentials and benefits of dynamic biogas production from Anaerobic Digestion (AD) of sewage sludge. The biogas production rate was aimed to match the flexible demand for electricity generation and so appropriate feeding regimes were calculated and tested in both pilot and demonstration scale. The results demonstrate that flexibilization capability exists for both conventional AD and advanced AD using Thermal Hydrolysis Process (THP) as pre-treatment. Whilst the former provides lower capability, flexible biogas production was achieved by the latter, as it provides a quick response. In all scenarios, the value of the biogas converted into electricity is higher than with a steady operational regime, increasing by 3.6% on average (up to 5.0%) in conventional and by 4.8% on average (up to 7.1%) with THP. The process has proven scalable up to 18 m3 digester capacity in operational conditions like those in full scale. Osmotic Membrane Bioreactor (OMBR) is an emerging technology for wastewater treatment with membrane fouling as a major challenge. This study aims to develop Adaptive Network-based Fuzzy Inference System (ANFIS) and Artificial Neural Network (ANN) models in simulating and predicting water flux in OMBR. Mixed liquor suspended solid (MLSS), electrical conductivity (EC) and dissolved oxygen (DO) were used as model inputs. Good prediction was demonstrated by both ANFIS models with R2 of 0.9755 and 0.9861, and ANN models with R2 of 0.9404 and 0.9817, for thin film composite (TFC) and cellulose triacetate (CTA) membranes, respectively. The root mean square error for TFC (0.2527) and CTA (0.1230) in ANFIS models was lower than in ANN models at 0.4049 and 0.1449. Sensitivity analysis showed that EC was the most important factor for both TFC and CTA membranes in ANN models, while EC (TFC) and MLSS (CTA) are key parameters in ANFIS models. Agricultural drainage ditches can prevent flooding and mitigate agricultural pollution; however, the performance is unsatisfactory in plateau areas like the Dianchi Lake basin. Thus, a novel double-layer ditch system (DDS) with a fibrous packing as biofilm carriers was developed to form the carrier-attached biofilms and enhance the pollutant removal. The results indicated the DDS performed better than a single-layer ditch system, and annual average removal efficiencies of TN, NO3--N, NH4+-N, TP, COD and SS were 18.61%, 17.13%, 7.74%, 11.90%, 11.95% and 23.71%, respectively. High amount and carbon, nitrogen and phosphorus contents of biofilms are favourable to pollutant removal by DDS. Although bacterial diversity of biofilms remained relatively stable throughout the year, the relative abundance of dominant assemblages varied greatly. Denitrifying microorganisms affiliated with Bacteroidetes might contribute to effective NO3--N reduction. This study demonstrates DDS performed well and provides a novel method for application of biofilm carriers in drainage ditches. The objective of this study was to evaluate three most common scale-up criteria for Haematococcus pluvialis production from cultivation bottles to 2 and 10 L of stirred tank PBRs. Constant volumetric power input (P/V) was found to be the most suitable criterion for H. pluvialis production. Total carotenoid amount per biomass concentration in 2 L and 10 L stirred tank PBRs were determined to be 4.57 mg/g and 4.77 mg/g, respectively. Antioxidant activity of total carotenoids extracted from H. pluvialis was also higher at constant P/V criterion where 46.91% inhibition rate with a total phenolic content of 11.76 mg gallic acid/L was achieved. Emricasan nmr Obtained results could be used to expand the bioproduction of H. pluvialis and its extracts in commercial scale. ETHNOPHARMACOLOGICAL RELEVANCE The effective-component compatibility of Bufei Yishen formula I (ECC-BYF I), a combination of 10 compounds, including total ginsenosides, astragaloside IV, icariin, and paeonol, etc., is derived from Bufei Yishen formula (BYF). The efficacy and safety of ECC-BYF I is equal to BYF. However, the composition of ECC-BYF I needs to be further optimized. Based on the beneficial effects of BYF and ECC-BYF I on chronic obstructive pulmonary disease (COPD), this study aimed to optimize the composition of ECC-BYF I and to explore the effects and mechanisms of optimized ECC-BYF I (ECC-BYF II) on mucus hypersecretion in COPD rats. MATERIALS AND METHODS ECC-BYF I was initially optimized to six groups optimized ECC-BYF I (OECC-BYF I)-A~F. Based on a COPD rat model, the effects of OECC-BYF I-A~F on COPD rats were evaluated. R-value comprehensive evaluation was used to evaluate the optimal formula, which was named ECC-BYF II. The changes in goblet cells and expression of mucins and the mRNA anded mucus hypersecretion in COPD rats, which manifested as reducing the expression of MUC5AC and MUC5B and the hyperplasia rate of goblet cells. The mRNA and protein expression levels of EGFR, PI3K, Akt, and mTOR were increased in COPD rats and were obviously downregulated after ECC-BYF II administration. CONCLUSION ECC-BYF II, which consists of paeonol, icariin, nobiletin, total ginsenoside and astragaloside IV, has beneficial effects equivalent to BYF and ECC-BYF I on COPD rats. ECC-BYF II significantly inhibited mucus hypersecretion, which may be related to the regulation of the EGFR/PI3K/mTOR pathway. OBJECTIVES To assess the validity of a bench-top model of an optical tympanometry device to diagnose in vitro model of middle ear effusion (MEE). METHODS AND MATERIALS We illuminated an in vitro model of ear canal and tympanic membrane with broadband light and relayed remitted light to a spectrometer system. We then used our proprietary algorithm to extract spectral features that, together with our logistic regression classifiers, led us to calculate a set of simplified indices related to different middle ear states. Our model included a glass vial covered with a porcine submucosa (representing the tympanic membrane) and filled with air, water, or milk solution (representing different MEE), and a set of cover-glass slips filled with either blood (representing erythema) or cerumen. By interchanging fluid types and cover-glass slips, we made measurements on combinations corresponding to normal healthy ear and purulent or serous MEE. RESULTS Each simulated condition had a distinct spectral profile, which was then employed by our algorithm to discriminate clean and cerumen-covered purulent and serous MEE. Two logistic purulent and serous MEE classifiers correctly classified all in vitro middle ear states with 100% accuracy assessed by leave-one-out and k-fold cross validation. CONCLUSIONS This proof-of-concept in vitro study addressed an unmet need by introducing a device that easily and accurately can assess middle ear effusion. Future in vivo studies aimed at collecting data from clinical settings are warranted to further elucidate the validity of the technology in diagnosing pediatric acute otitis media. V.Congenital external auditory canal stenosis (EACS) is a spectrum of abnormalities affecting the external and middle ear. We report a 6 year-old patient with EACS affecting the lateral fibrocartilaginous canal that was successfully repaired. This patient highlights a variant of EACS characterized by lateral soft tissue narrowing with normal osseous development. Most previous studies of CAA have described severe forms associated with complete atresia, bony stenosis, and middle ear malformations. Stenosis affecting only the fibrocartilaginous canal is a milder form resulting from premature arrest of the canalization process during embryologic development, and may predispose to cholesteatoma formation. HYPOTHESIS The development of advanced oral delivery systems for bioactive compounds requires the fundamental understanding of the digestion process within the gastrointestinal tract. Towards this goal, dynamic invitro digestion models, capable of characterising the molecular as well as colloidal aspects of food, together with their biological interactions with relevant invitro cell culture models, are essential. EXPERIMENTS In this study, we demonstrate a novel digestion model that combines flow-through time resolved small angle X-ray scattering (SAXS) with an invitro Caco-2/HT-29 cell co-culture model that also contained a mucus layer. This set-up allows the dynamic insitu characterisation of colloidal structures and their transport across a viable intestinal cell layer during simulated digestion. FINDINGS An integrated online SAXS - invitro cell co-culture model was developed and applied to study the digestion of nature's own emulsion, milk. The impact of the invitro cell culture on the digestion-triggered formation and evolution of highly ordered nanostructures in milk is demonstrated.