Pallesenwall9910

Mono- and diacylglycerol (MAG and DAG) emulsifiers (E 471) are widely applied to regulate techno-functional properties in different food categories, for example, in dairy products. A method for the determination of MAG and DAG in aerosol whipping cream by high-performance thin-layer chromatography with fluorescence detection (HPTLC-FLD) after derivatization with primuline was developed. For sample preparation, aerosol whipping cream was mixed with ethanol, followed by the addition of water and liquid-liquid extraction with tert-butyl methyl ether. The sample extracts were analyzed by HPTLC-FLD on silica gel LiChrospher plates with n-pentane/n-hexane/diethyl ether (22.522.555, v/v/v) as mobile phase, when interfering matrix like cholesterol and triacylglycerols were successfully separated from the E 471 food additives. For quantitation, an emulsifier with known composition was used as calibration standard and the fluorescent MAG and DAG were scanned at 366/> 400 nm. Limits of detection and quantitation of 4 and 11 mg/100 g aerosol whipping cream were obtained for both monostearin and 1,2-distearin, respectively, and allowed the reliable quantitation of MAG and DAG from E 471 far below commonly applied emulsifier amounts. Recoveries from model aerosol whipping cream with 400 mg E 471/100 g were determined in a calibration range of 200-600 mg E 471/100 g sample and ranged between 86 and 105% with relative standard deviations below 7%. In aerosol whipping creams from the German market, E 471 amounts ranged between 384 and 610 mg/100 g.Multi-walled carbon nanotubes (MWCNT) play a synergistic role with conducting polymer in practical applications such as biological sensing. In this paper, multi-walled carbon nanotube and polypyrrole (PPy) composites were prepared on a fiber surface for the first time, and their morphology and electrical properties were characterized. Compared with PPy-coated fiber, the presence of carbon nanotubes induced the growth of large areas of PPy nanowires. In addition, fiber organic electrochemical transistors (FECTs) based on PPy and MWCNT were assembled, showing a higher on/off ratio, better stability, and greater flexibility. The lactate biosensor based on FECTs exhibits high sensitivity, with a correlation coefficient of R = 0.9889, quick response time of 0.6-0.8 s, a wide linear response range of 1 nM-1 mM, and excellent selectivity for lactate. Furthermore, the lactate concentration in human sweat was successfully detected by a FECT-based sensor. The hybrid fibers can be easily woven and placed on fabric simply by stitching. This favorable performance of the FECT-based sensor makes it suitable for noninvasive sensing of lactate. Therefore, it provides a promising platform for future use in healthcare and detection applications. Graphical abstract.Zinc (Zn) is an essential micronutrient, and Zn deficiency remains a major global public health challenge. Recognised biomarkers of population Zn status include blood plasma or serum Zn concentration and proxy data such as dietary Zn intake and prevalence of stunting. Urine Zn concentration is rarely used to assess population Zn status. This study assessed the value of urine Zn concentration as a biomarker of population Zn status using a nationally representative sample of non-pregnant women of reproductive age (WRA) and school-aged children (SAC) in Malawi. Spot (casual) urine samples were collected from 741 WRA and 665 SAC. Urine Zn concentration was measured by inductively coupled plasma mass spectrometry with specific gravity adjustment for hydration status. Data were analysed using a linear mixed model with a spatially correlated random effect for between-cluster variation. The effect of time of sample collection (morning or afternoon), and gender (for SAC), on urine Zn concentration were examined. There was spatial dependence in urine Zn concentration between clusters among SAC but not WRA, which indicates that food system or environmental factors can influence urine Zn concentration. Mapping urine Zn concentration could potentially identify areas where the prevalence of Zn deficiency is greater and thus where further sampling or interventions might be targeted. There was no evidence for differences in urine Zn concentration between gender (P = 0.69) or time of sample collection (P = 0.85) in SAC. Urine Zn concentration was greater in afternoon samples for WRA (P = 0.003). Relationships between urine Zn concentration, serum Zn concentration, dietary Zn intake, and potential food systems covariates warrant further study.Soil contamination by heavy metals (HM) is a worldwide problem for human health. To reduce risk to human health from exposure to toxic chemicals associated with soil contamination, it is necessary to monitor and assess HM concentrations in the soil for places where the concentration exceeds the acceptable levels. Spatial patterning is a necessary tool for assessment of the exposure risk of HM contamination. Soil sampling (n = 65) was carried out in technogenically polluted soils located at Rostov oblast to study the content and spatial distribution of four HM (Cu, Zn, Pb, and Cr) in the surface layer (0-20 cm) of the impact zone of former Lake Atamanskoe (floodplain of the Seversky Donets River valley, Rostov region) with an area of 3.91 km2. Extremely high values of HM concentrations were found with the maximum values of 702 mg/kg, 72,886 mg/kg, 2300 mg/kg, 259 mg/kg for Cu, Zn, Pb, and Cr, respectively. Inverse distance-weighted (IDW) interpolation was used to prepare 3D monoelement images of HM. Lognormal kriging and indicator kriging techniques were applied to create elemental spatial distribution maps and HM probability maps. The results showed that the total content of Cu, Zn, Pb, and Cr was moderately spatially dependent (nugget-to-sill ratio ranged from 31 to 38%), whereas the contamination index Zc formed strong spatial dependence patterns (nugget-to-sill ratio ranged from 0 to 21.4%). The obtained results of this study could serve as a guide to the authorities in identifying those areas which need remediation. Moreover, this study provides a tool for assessing the hygienic situation in the vicinity of Kamensk-Shakhtinsky (Rostov region) for decision making that can help to minimize the environmental risk of technogenic soil contamination of HM.

Topical administration of tranexamic acid (TXA) in patients undergoing total knee arthroplasty (TKA) is increasingly popular as it avoids the risks related with systemic absorption of the medication. Previous studies have established the efficacy of TXA in TKA, however here are limited direct comparison studies available and the dosing regimens vary. Hence, there is no consensus on an optimal dose. Our objective is to compare blood loss, transfusion requirement and immediate post-operative function between high (2g) and low (1g) dose tranexamic acid in patients undergoing TKA.

This is a retrospective cohort study of 104 patients undergoing total knee arthroplasty in a single institution under a single surgeon. In total, 61 and 43 patients receiving 1g and 2g of topical TXA respectively. Blood loss as estimated from the difference in haemoglobin (Hb) and haematocrit (HCT) levels post-surgery and number of blood transfusions required were compared between groups. Immediate post-operative function and complications were also measured.

Patient characteristics were mostly similar between groups. The transfusion requirements were higher in 1g group compared to the 2g group (0.11 vs 0.00, p = 0.034). The mean post op day 1 (POD1) range of motion higher in the 1g group vs 2g group (72.1 vs 63.7, p = 0.035). The 2g group had a lower POD1 pain score compared to the 1g group (4.02 vs 5.43, p < 0.01). There was no statistically significant difference in complications that were related to the administration of TXA between the two groups.

Higher dose of topical TXA is safe, helps improve immediate post-operative functional outcomes and reduces transfusion requirements.

Higher dose of topical TXA is safe, helps improve immediate post-operative functional outcomes and reduces transfusion requirements.

Various orthopedic surgical procedures cause mechanical stress for gloves. In some cases, sharp-edged objects impact on the glove surfaces. The systematic description of lesions is still missing.

2289 gloves from 409 surgeries [primary hip and knee arthroplasties (PA), revisions arthroplasties (RA) and arthroscopic shoulder, hip and knee surgery (AY)] from 3 clinics were examined for lesions using water tightening test according to the European norm EN 455-1.

Arthroscopies showed the lowest rate of operations with damaged gloves (6.9%). Depending on clinic, 32.7% and 59.2% of PA surgeries generated damaged gloves, while in RA, these numbers rose to 76.0% and 72.8%, respectively. In PA and RA, the most affected finger was the index finger, whereas in arthroscopies, more damage occurred on the middle finger and the thumb. The size of the lesions was rather small with the vast majority being 1mm or 2mm in size.

All investigated interventions led to glove lesions. With increasing mechanical stress, the number of glove defects increased. EN 455 does not account for the intraoperative tear risk. Stricter requirements for gloves should be introduced. Glove change intervals should be defined and implemented, and new materials should be developed.

All investigated interventions led to glove lesions. With increasing mechanical stress, the number of glove defects increased. EN 455 does not account for the intraoperative tear risk. Stricter requirements for gloves should be introduced. Glove change intervals should be defined and implemented, and new materials should be developed.

Thirty-day mortality of patients with hip fracture is well researched and predictive; validated scoring tools have been developed (Nottingham Hip Fracture Score, NHFS). COVID-19 has significantly greater mortality in the elderly and comorbid patients which includes hip fracture patients. Non-operative treatment is not appropriate due to significantly higher mortality, and therefore, these patients are often exposed to COVID-19 in the peri-operative period. What is unclear is the effect of concomitant COVID-19 infection in these patients.

A multicentre prospective study across ten sites in the United Kingdom (responsible for 7% of hip fracture patients per annum in the UK). Demographic and background information were collected by independent chart review. Data on surgical factors included American Society of Anesthesiologists (ASA) score, time to theatre, Nottingham Hip fracture score (NHFS) and classification of fracture were also collected between 1st March 2020 and 30th April 2020 with a matched cohort severity of COVID-19 infection in surgical patients and the potential impact of COVID-19 on elective surgical patients in the peri-operative period.Here, we investigated the effects of total dietary fish oil (FO) substitution with plant oil (PO) on hematological indices, immune status, antioxidant activity, IL1β and TNF-α gene expression, and hypoxia stress resistance in Oreochromis niloticus at suboptimal temperatures. Fish (n = 360) were randomly divided into 12 circular fiberglass tanks (500 L; 3 replicates for each dietary group, 30 fish/replicate, 90 fish/group). The control group was fed a basal diet with FO as the lipid source. The CO, SFO, and LSO groups were fed a basal diet with complete replacement of FO with corn, sunflower, and linseed oils, respectively. After 4 weeks, no effects on hematological indices were observed in fish fed the experimental diets. The LSO and CO groups showed a significant increase in γ globulin levels. The highest levels of non-specific immune parameters, antioxidant activity, and IL-1β and TNF-α gene expressions were recorded in the LSO group. The LSO group also exhibited good resistance to hypoxia stress. Therefore, the total dietary substitution of FO with PO (especially LSO and CO) is recommended as a valuable strategy to ameliorate the immunosuppressive effects of suboptimal temperatures and enhance the resistance of O.