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If these cases are included, the incidence of TSD in the French-Canadian population of Quebec is 1/169,668 and the carrier frequency 1/206. These findings can be used for genetic counseling and policy decisions regarding carrier screening for TSD in populations of French-Canadian descent. © 2020 National Society of Genetic Counselors.Stanozolol is still the most commonly used illicit anabolic-androgenic steroid (AAS) in professional sports. Therefore, accurate and fast analysis and long detection windows are of high interest in the field of anti-doping analysis. In this work, a very simple, fast and highly sensitive online solid-phase extraction method coupled with liquid chromatography - high-resolution tandem mass spectrometry (HPLC-HRMSMS) for the analysis of stanozolol-N-glucuronides was developed. This fully validated procedure is characterized by only a few manual steps (dilution and addition of internal standard) in the sample preparation. A limit of identification (LOI) of 75 pg/ml, high accuracy (87.1% - 102.1%), precision (3.1% - 7.8%) and sensitivity was achieved. Furthermore, good linearity (&gt; 0.99) and robustness, as well as no carry-over effects, could be observed. In addition to excellent confirmation analysis performance, this method shows sufficient potential for the identification and characterization of unknown metabolites. Using this method, it was possible to unambiguously confirm the presence of 1'N- and 2'N-stanozolol-glucuronide in human urine for the first time due to the access to reference material. This article is protected by copyright. All rights reserved.Avian leukosis virus (ALV) is the cause of a variety of tumor diseases in poultry, causing huge economic losses all over the world. As a retrovirus, its genome is highly variable and easily recombined between different subgroups. Previous studies found several recombinant events among subgroup A, B, and E, but few data show that in subgroup J, which is the most prevalent and pathogenic in chickens. This study identified and sequenced an emerging recombinant ALV from yellow chicken, and analysis showed that the homology between the env gene of the new isolate and the representative strain (BR119) of subgroup J is as high as 98.7%, while its long-terminal-repeat (LTR) was highly consistent with some representative strains of subgroup E (94.9%-98.4%). This study also found that such LTR has appeared in some published strains of subgroup B and subgroup K, indicating that it can combine with the coding region of many different subgroups of ALV, thus forming natural recombinant strains. At the same time, a pair of polymerase chain reaction (PCR) primers were selected according to the sequence, which can specifically detect the recombinant strains in wild, facilitating the future ALV eradication program. Overall, this study reminds us to pay more attention to tracking the genome variation of avian leukosis virus in the future. This article is protected by copyright. All rights reserved.Giardia duodenalis and Cryptosporidium spp. are ubiquitous intestinal protozoa that parasitize domestic and wild animals, as well as human beings. Due to their zoonotic potential, the objective of the present study was to determine the presence of these pathogens in the fox population (Vulpes vulpes) located in Northwest Spain. A total of 197 faecal samples from legally hunted foxes were collected in the autonomous region of Galicia. The presence of G. duodenalis and Cryptosporidium spp. was investigated by PCR-based methods amplifying the small subunit ribosomal RNA (ssu rRNA) gene of the parasites. Attempts to genotype obtained positive samples were subsequently conducted at the glutamate dehydrogenase (gdh) and β-giardin (bg) genes of G. duodenalis, and the 60&nbsp;kDa glycoprotein (gp60) gene of Cryptosporidium. Giardia duodenalis and Cryptosporidium spp. were identified in 19 (9.6%) and 12 (6.1%) of the investigated samples, respectively. However, five Cryptosporidium species were detected at the ssu rRNA locus C. hominis (33.4%, 4/12), C. canis (25.0%, 3/12), C. parvum (16.7%, 2/12), C. ubiquitum (8.3%, 1/12) and C. suis (8.3%, 1/12). An additional Cryptosporidium-positive sample was identified at the genus level only. Typing and subtyping of Giardia- and Cryptosporidium-positive samples were unsuccessful. The detection of C. hominis in wild foxes indicates the probable overlapping of sylvatic and domestic cycles of this parasite in rural settings. Besides, this finding raises the question of whether red foxes may act as natural reservoirs of C. hominis. The detection of C. parvum and C. suis is suggestive of active transmission events between farm and wild animals, opening up the possibility of transmission to human beings. © 2020 Blackwell Verlag GmbH.We report a comprehensive fragment library with 205,903 fragments derived from the recently published Collection of Open Natural Products (COCONUT) data set with more than 400,000 non-redundant natural products. The natural products-based fragment library was compared with other two fragment libraries herein generated from ChEMBL (biologically relevant compounds) and Enamine-REAL (a large on-demand collection of synthetic compounds), both used as reference data sets with relevance in drug discovery. It was found that there is a large diversity of unique fragments derived from natural products and that the entire structures and fragments derived from natural products are more diverse and structurally complex than the two reference compound collections. During this work we introduced a novel visual representation of the chemical space based on the recently published concept of statistical-based database fingerprint. The compounds and fragments libraries from natural products generated and analyzed in this work are freely available. © 2020 Wiley-VCH Verlag GmbH &amp; Co. KGaA, Weinheim.The coronavirus 2 (SARS-CoV-2) pandemic represents and will represent the greatest health, economic and social crisis of the new millennium. According to the World Health Organization (WHO) report published on April 14, 2020, the pandemic SARS-CoV-2 affects 213 countries worldwide, with 1,848,439 infected patients and 117,217 confirmed deaths (https//www.who.int/emergencies/diseases/novel-coronavirus-2019) to this date. [https://www.selleckchem.com/products/msc2530818.html selleck products] Men are much more prone to become seriously ill than women. Fortunately, the majority of men, especially in younger age, survive the infection. 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