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We report the case of a 36-year-old health care worker who suffered a subacute infarct with a mild deviation of the midline, and a large vessel occlusion with a free-floating thrombus in the ascending aorta in the context of a SARS-CoV-2 infection. With this case we want to increase the awareness about severe forms of systemic ischemia and stroke in patients with signs of COVID infection. This article is protected by copyright. All rights reserved.OBJECTIVE The present case-control study aims to compare the psychological status of patients with and without halitosis, and investigate the association of psychological disorders and halitosis. METHODS Patients who complained about bad breath and diagnosed with genuine halitosis were assigned to the halitosis group, while patients without genuine halitosis were assigned into the control group (n=106, each group). Information on the demographics and Symptom Checklist-90 (SCL-90) of participants were collected. The organoleptic score and Halimeter measurement were used to measure halitosis. RESULTS The mean SCL-90 score of participants in the halitosis group (0.63) was significantly greater than that in the control group (0.48) (P=0.002). The scores of the domains of interpersonal sensitivity, anxiety, depression and paranoid ideation were found to be significantly different between two groups (P less then 0.05). However, there were no significant differences in SCL-90 scores between the mild and moderate-severe halitosis groups (P=0.479). CONCLUSION The psychological status of genuine halitosis patients was significantly worse compared to normal patients without halitosis complaint and also without halitosis. The main problems were in the aspects of interpersonal sensitivity, anxiety, depression and paranoia. However, the negative impact was not related with the severity of halitosis. This article is protected by copyright. All rights reserved.BACKGROUND Hyperglycemia in acute stroke leads to poor neurological outcomes. The role of microRNA (miRNA) in hyperglycemia-associated genes can provide new avenues for stroke prognostic applications. We aimed to identify novel genes and their regulated miRNAs that are associated with hyperglycemia-induced unfavorable stroke outcomes and further validated in the plasma exosome. Moreover, we intended to evaluate the prognostic ability of miRNA-messenger RNA (mRNA) biomarkers in addition to using traditional risk factors. METHODS After the integration analysis of small RNA sequencing and mRNA PCR array, two mRNAs and six miRNAs were selected for validation in middle cerebral artery occlusion animal models and ischemic stroke (IS) patients. Receiver operator characteristic (ROC) analysis was used to determine the performance of mRNAs and miRNAs expression. RESULTS The increased Fas expression was associated with hyperglycemia after acute stroke onset in animal and human studies. Besides, Fas gene level was significantly higher in unfavorable outcome patients when compared to favorable outcome patients. The expression of Fas and miRNA hsa-let-7b-5p in addition to traditional risk factors could increase the discrimination and predictive ability for poor prognosis. The higher exosomal Fas was further observed among unfavorable outcome patients, suggesting Fas signal transporting through exosome in the circulating system. CONCLUSIONS Combined analyses of Fas and has-let-7b-5p expressions in addition to traditional risk factors are favorable prognostic biomarkers for predicting poor neurological outcomes at 3 months after stroke onset in IS patients. Additional studies are required to address the precise role of the apoptosis pathway in unfavorable hyperglycemia-induced stroke outcomes. This article is protected by copyright. All rights reserved.Coronavirus disease 2019 (COVID-19), caused by a novel coronavirus, SARS-COV-2 was declared a Public Health Emergency of&nbsp;International Concern (PHEIC) by the World Health Organization (WHO) in January 2020. Human-to-Human transmission occurs through close contact with an infected person or surfaces that are contaminated with droplets or secretions. This article is protected by copyright. All rights reserved.BACKGROUND The actin bundling protein Fascin is essential for developmental cell migrations and promotes cancer metastasis. In addition to bundling actin, Fascin has several actin-independent roles; how these other functions contribute to cell migration remains unclear. Border cell migration during Drosophila oogenesis provides an excellent model to study Fascin's various roles during invasive, collective cell migration. RESULTS On-time border cell migration during Stage 9 requires Fascin (Drosophila Singed). Fascin functions not only within the migrating border cells, but also within the nurse cells, the substrate for this migration. Fascin genetically interacts with the actin elongation factor Enabled to promote on-time Stage 9 migration and overexpression of Enabled suppresses the defects seen with loss of Fascin. Loss of Fascin results in increased, shorter and mislocalized protrusions during migration. Additionally, loss of Fascin inhibits border cell delamination and increases E-Cadherin (Drosophila Shotgun) adhesions on both the border cell clusters and nurse cells. CONCLUSIONS Overall, Fascin promotes on-time border cell migration during Stage 9 and contributes to multiple aspects of this invasive, collective cell migration, including both protrusion dynamics and delamination. These findings have implications beyond Drosophila, as border cell migration has emerged as a model to study mechanisms mediating cancer metastasis. This article is protected by copyright. All rights reserved. © 2020 Wiley Periodicals, Inc.OBJECTIVE The gold standard for measuring blood-retinal barrier permeability is the Evans blue assay. [https://www.selleckchem.com/products/PD-0332991.html this website] However, this technique has limitations in vivo, including non-specific tissue binding and toxicity. This study describes a non-toxic, high throughput and cost effective alternative technique that minimizes animal usage. METHODS Sodium fluorescein fundus angiography was performed in non- and diabetic Brown Norway rats on days 0, 7, 14, 21 and 28. Sodium fluorescein intensity in the retinal interstitium and a main retinal vessel were measured over time. The intensity gradients were used to quantify retinal vascular permeability. Post study eyes were fixed, dissected and stained (isolectin B4) to measure required parameters for permeability quantification including Total vessel length per retinal volume, radius and thickness. RESULTS In the non-diabetic cohort retinal permeability remained constant over the 28-day study period. However, in the diabetic cohort there was a significant and progressive increase in retinal permeability from day 14 to 28 (p less then 0.