Zobrazení zdroje stránky Putnamdugan4242

Participants meeting criteria for depression were more likely to be taking regular mood stabiliser medications and to exhibit aggressive challenging behaviour. Participants meeting criteria for anxiety were more likely to have sleep difficulties and report loneliness. Participants meeting criteria for either/both depression and anxiety were more likely to report loneliness. [https://www.selleckchem.com/EGFR(HER).html https://www.selleckchem.com/EGFR(HER).html] CONCLUSIONS This study identified both treatable and modifiable, as well as unmodifiable, biopsychosocial factors associated with depression and/or anxiety in older adults with ID. A longitudinal study follow-up will further develop our knowledge on the causality and direction of associated biopsychosocial factors with depression and anxiety in older adults with ID and better inform management strategies, prevention policies and funding of services. © 2020 MENCAP and International Association of the Scientific Study of Intellectual and Developmental Disabilities and John Wiley &amp; Sons Ltd.The frequent occurrence of chalky rice (Oryza sativa L.) grains becomes a serious problem due to climate change. However, the molecular mechanism underlying chalkiness is largely unknown. In this study, the temperature-sensitive floury endosperm11-2 (flo11-2) mutant was isolated from ion beam-irradiated rice of 1,116 lines. The flo11-2 mutant showed significantly higher chalkiness under mean temperature of 28 °C than the wild type, but similar levels of chalkiness to the wild type under mean temperature of 24 °C. Whole exome sequencing of the flo11-2 mutant showed three causal gene candidates, including Os12g0244100, which encodes plastid-localized 70 kDa heat shock protein 2 (cpHSP70-2). The cpHSP70-2 of the flo11-2 mutant has an amino acid substitution on the 259th aspartic acid with valine (D259V) in the conserved Motif 5 of ATPase domain. Transgenic flo11-2 mutants that express the wild type cpHSP70-2 showed significantly lower chalkiness than the flo11-2 mutant. Moreover, the accumulation level of cpHSP70-2 was negatively correlated with chalky ratio, indicating that the cpHSP70-2 is a causal gene for the chalkiness of the flo11-2 mutant. The intrinsic ATPase activity of recombinant cpHSP70-2 was lower by 23% at Vmax in the flo11-2 mutant type than in the wild type. The growth of DnaK-defective Escherichia coli cells complemented with DnaK with D201V mutation (equivalent to the D259V mutation) was severely reduced at 37 °C, but not in the wild type DnaK. The results indicate that the lowered cpHSP70-2 function is involved with the chalkiness of the flo11-2 mutant. This article is protected by copyright. All rights reserved.Plant cell wall remodeling plays a key role in the control of cell elongation and differentiation. In particular, the fine-tuning of the degree of methylesterification of pectins was previously reported to control developmental processes as diverse as pollen germination, pollen tube elongation, primordia emergence or dark-grown hypocotyl elongation. How pectin degradation can modulate plant development has however remained elusive. Here, we report the characterization of a polygalacturonase (PG), AtPGLR, which gene is highly expressed at the onset of lateral root emergence in Arabidopsis. Due to gene compensation mechanisms, mutant approaches failed to determine the involvement of AtPGLR in plant growth. To overcome this issue, AtPGLR has been expressed heterologously in the yeast Pichia pastoris and biochemically characterized. We showed that AtPGLR is an endo-PG that preferentially releases non-methylesterified oligogalacturonides of short degree of polymerization ( less then 8) at acidic pH. The application of the purified recombinant protein on Amaryllis pollen tubes, an excellent model to study cell wall remodeling at acidic pH, induced abnormal pollen tubes or cytoplasmic leakage in the sub-apical dome of the pollen tube tip, where non-methylesterified pectin epitopes are detected. Those leaks could either be repaired by new β-glucan deposits (mostly callose) in the cell wall or promoted dramatic burst of the pollen tube. Our work presents the full biochemical characterization of an Arabidopsis PG and highlights the importance of pectin integrity in pollen tube elongation. This article is protected by copyright. All rights reserved.INTRODUCTION In 2014 and 2017, the Centers for Medicare and Medicaid Services authorized nutrition-related ordering privileges for registered dietitian nutritionists (RDNs) in hospital and long-term care settings, respectively. Despite this practice advancement, information describing current parenteral nutrition (PN) and enteral nutrition (EN) ordering practices is lacking. Dietitians in Nutrition Support, a dietetic practice group of the Academy of Nutrition and Dietetics and the Dietetics Practice Section of the American Society of Parenteral and Enteral Nutrition (ASPEN) utilized a survey to describe PN and EN ordering practices among RDNs in the United States. METHODS A cross-sectional study design was utilized to describe RDN PN and EN ordering privileges. Respondents were asked to describe PN and EN ordering privileges, primary practice setting, primary patient population served, nutrition specialty certification, highest degree earned, career length, and if applicable, the nature of prior denials for esearch and the ASPEN Board of Directors. This article has been co-published with permission in the Journal of the Academy of Nutrition and Dietetics. The articles are identical except for minor stylistic and spelling differences in keeping with each journal's style. Either citation can be used when citing this article. © 2020 American Society for Parenteral and Enteral Nutrition.OBJECTIVE The mechanisms by which antiepileptic drugs (AEDs) cause birth defects (BDs) are unknown. Data suggest that AED-induced BDs may result from a genome-wide increase of de novo variants in the embryo, a mechanism which we investigated. METHODS Whole-exome sequencing data from child-parent trios were interrogated for de novo single-nucleotide variants/indels (dnSNVs/indels) and copy number variants (dnCNVs). Generalized linear models were applied to assess de novo variant burdens in children exposed prenatally to AEDs (AED-exposed children) vs children without BDs not exposed prenatally to AEDs (AED-unexposed unaffected children), and AED-exposed children with BDs vs those without BDs, adjusting for confounders. Fisher's exact test was used to compare categorical data. RESULTS 67 child-parent trios were included 10 with AED-exposed children with BDs; 46 with AED-exposed unaffected children; 11 with AED-unexposed unaffected children. The dnSNV/indel burden did not differ between AED-exposed children and AED-unexposed unaffected children [median dnSNV/indel number/child (range) 3 (0-7) vs 3 (1-5), p&nbsp;=&nbsp;0.