Zobrazení zdroje stránky Powellcook9294

Viscosity is an important parameter for evaluating cell health, and abnormal viscosity can cause a variety of intracellular organelle function disorders. The mitochondria are a key organelle in cells, and the viscosity of the mitochondria determines the state of the cell. In this work, we report a novel near-infrared fluorescent probe, referred to as NI-VD, that has a large Stokes-shift and a satisfactory response multiple. NI-VD can sensitively detect changes in cell viscosity in cells and tissues, and it can effectively avoid interference from the overlap of excitation and emission light. The fluorescence spectrum shows that NI-VD has maximum emission peaks at 730 nm, and the fluorescence intensity is amplified with an increase in the solution viscosity. The response from pure PBS solution to glycerol changes by 13-fold. After confirmation in a variety of cell and biological models, NI-VD can detect the changes in viscosity in mitochondria. Most importantly, this study is the first to visualize the differences between the kidneys of diabetic mice and normal mice. This approach is a new solution for the diagnosis and treatment of diabetic nephropathy.Histone deacetylases (HDACs) have been implicated in a number of diseases including cancer, cardiovascular disorders, diabetes mellitus, neurodegenerative disorders and inflammation. For the treatment of epigenetically altered diseases such as cancer, HDAC inhibitors have made a significant progress in terms of development of isoform selective inhibitiors. Isoform specific HDAC inhibitors have less adverse events and better safety profile. A HDAC isoform i.e., HDAC2 demonstrated significant role in the development of variety of diseases, mainly involved in the cancer and neurodegenerative disorders. Discovery and development of selective HDAC2 inhibitors have a great potential for the treatment of target diseases. In the present compilation, we have reviewed the role of HDAC2 in progression of cancer and neurodegenerative disorders, and information on the drug development opportunities for selective HDAC2 inhibition.Engineered small graphene oxide (s-GO) sheets were previously shown to reversibly down-regulate glutamatergic synapses in the hippocampus of juvenile rats, disclosing an unexpected translational potential of these nanomaterials to target selective synapses in vivo. Synapses are anatomical specializations acting in the Central Nervous System (CNS) as functional interfaces among neurons. Dynamic changes in synaptic function, named synaptic plasticity, are crucial to learning and memory. More recently, pathological mechanisms involving dysfunctional synaptic plasticity were implicated in several brain diseases, from dementia to anxiety disorders. Hyper-excitability of glutamatergic neurons in the lateral nucleus of the amygdala complex (LA) is substantially involved in the storage of aversive memory induced by stressful events enabling post-traumatic stress disorder (PTSD). Here we translated in PTSD animal model the ability of s-GO, when stereotaxically administered to hamper LA glutamatergic transmission and to prevent the behavioral response featured in long-term aversive memory. We propose that s-GO, by interference with glutamatergic plasticity, impair LA-dependent memory retrieval related to PTSD.Membrane fouling by influent biopolymers, and the formation of surface biofilms, are major obstacles to the practical application of membrane technologies. Identifying reliable and sustainable pre-treatment methods for membrane filtration remains a considerable challenge and is the subject of continuing research study worldwide. Herein, the performance of a bench-scale gravity-driven up-flow slow biofilter (GUSB) as the pre-treatment for ultrafiltration to reduce membrane fouling is presented. Dissolved organic carbon (DOC) was shown efficiently removed by the GUSB (around 80%) when treating a natural water influent. More significantly, biopolymers, with molecular weight (MW) between 20 kDa and 100 kDa, were effectively removed (62.8% reduction) and this led to a lower rate of transmembrane pressure (TMP) development by the UF membrane. Microbial diversity analysis further unraveled the function of GUSB in shaping microbes to degrade biopolymers, contributing to lower accumulation and different distribution pattern of SMP on the membrane surface. Moreover, the biofilm formed on the membrane surface after the pre-treatment of GUSB was observed to have a relative porous structure compared to the control system, which can also affect the fouling development. Long-term operation of GUSB further revealed its robust performance in reducing both natural organic matters and UF fouling propensity. This study overall has demonstrated the potential advantages of applying a GUSB to enhance UF process performance by reducing biofouling and improving effluent quality.Elemental sulfur (S0) reduction process has been demonstrated as an attractive and cost-efficient approach for metal-laden wastewater treatment in lab-scale studies. However, the system performance and stability have not been evaluated in pilot- or large-scale wastewater treatment. Especially, the sulfide production rate and microbial community structure may significantly vary from lab-scale system to pilot- or large-scale systems using real domestic sewage as carbon source, which brings questions to this novel technology. In this study, therefore, a pilot-scale sulfur-based sulfidogenic treatment system was newly developed and applied for the treatment of Cu-laden electroplating wastewaters using domestic sewage as carbon source. During the 175-d operation, &gt;99.9% of Cu2+ (i.e., 5580 and 1187 mg Cu/L for two types of electroplating wastewaters) was efficiently removed by the biogenic hydrogen sulfide that produced through S0 reduction. Relatively high level of sulfide production (200 mg S/L) can be achieved ation tank. Compared with other reported technologies, the sulfur-based treatment system remarkable reduced the total chemical cost by 87.5‒99.6% for the same level of Cu2+ removal. [https://www.selleckchem.com/products/td139.html selleck chemicals llc] Therefore, this pilot-scale study demonstrated that S0 reduction process can be a sustainable technology to generate sulfide for the co-treatment of Cu-laden electroplating wastewater and domestic sewage, achieving higher Cu2+removal and higher cost-effectiveness than the conventional technologies.