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Objectives To establish whether blood samples taken from used peripheral intravenous cannulae are clinically interchangeable with venepuncture. Design Systematic review. PubMed, Web of Science and Embase were searched for relevant trials. Setting Trials which compared blood samples from used peripheral intravenous cannulae to venepuncture and provided limits of agreement or data which allowed calculation of limits of agreement. Participants Seven trials with 746 participants. Blood tests included 13 commonly ordered biochemistry, haematology and blood gas measurements. Main outcome measures 95% limits of agreement. Data were pooled using inverse variance weighting and compared to a clinically acceptable range estimated by expert opinion from previous trials. Results Limits of agreement for blood samples from used peripheral intravenous cannulae were within the clinically acceptable range for sodium, chloride, urea, creatinine and haematology samples. Limits of agreement for potassium were ±0.47 mmol/L which exceeded the clinically acceptable range. Peripheral intravenous cannula samples for blood gas analysis gave limits of agreement which far exceeded the clinically acceptable range. Conclusions Blood sampling from used peripheral intravenous cannulae is a reasonable clinical practice for haematology and biochemistry samples. Potassium samples from used peripheral intravenous cannulae can be used in situations where error up to ±0.47 mmol/L is acceptable. Peripheral intravenous cannula samples should not be used for blood gas analysis.Objective Using data from 40 years of national surveys of UK medical graduates, we report on ophthalmology as a career choice. Design setting and participants Self-administered questionnaire surveys of all graduates from all UK medical schools in selected years of qualification between 1974 and 2015. Main outcome measures Career specialty preferences of doctors one, three, and five years after graduation; career specialty destinations 10 years after graduation. Results One year after graduation, ophthalmology was the first career preference of 1.6% of the qualifiers of 1974-83, 2.2% of 1993-2002, and 1.8% of 2005-15. The corresponding percentages three years after graduation were 1.5, 1.8, and 1.2%. Men were more likely than women to choose ophthalmology among graduates of 2005-15, 2.4% of men and 1.4% of women did so at one year, as did 1.7% of men and 0.7% of women at five years. Seventy per cent of doctors practising as ophthalmologists 10 years after qualification had told us in their first post-qualification year that ophthalmology was their first choice of career. Conclusions There has been no systematic change in recent years in the proportion of recent medical graduates intending to have a career in ophthalmology when surveyed one year after graduation. However, the proportion at three and five years after graduation was lower than that at year 1. Suggestions for maintaining interest in the specialty include improved career advice, greater early clinical exposure to ophthalmology, and improved access to flexible training. Most practising ophthalmologists had made early decisions that this was their intended career.Owing to their unique electrical and optical properties, two-dimensional transition metal dichalcogenides have been extensively studied for their potential applications in biosensing. However, simultaneous utilization of both optical and electrical properties has been overlooked, yet it can offer enhanced accuracy and detection versitility. Here, we demonstrate a dual-mode optoelectronic biosensor based on monolayer molybdenum disulfide (MoS2) capable of producing simultaneous electrical and optical readouts of biomolecular signals. On a single platform, the biosensor exhibits a tunable photonic Fano-type optical resonance while also functioning as a field-effect transistor (FET) based on a optically transparent gate electrode. Furthermore, chemical vapor deposition grown MoS2 provides a clean surface for direct immobilization of a water-soluble variant of the μ-opioid receptor (wsMOR), via a nickel ion-mediated linker chemistry. We utilize a synthetic opioid peptide to show the operation of the electronic and optical sensing modes. The responses of both modes exhibit a similar trend with dynamic ranges of four orders of magnitude and detection limits of less then 1 nM. Our work explores the potential of a versatile multimodal sensing platform enabled by monolayer MoS2, since the integration of electrical and optical sensors on the same chip can offer flexibility in read-out and improve the accuracy in detection of low concentration targets.Background Access to safe, effective, quality and affordable essential medicines for all is a central component of Universal Health Coverage (UHC). However, the availability of quality medicines in peripheral healthcare facilities is often limited. Several countries have developed integrated complementary pharmaceutical supply systems to address the shortage of medicines. Nevertheless, there is little evidence on how accountability contributes to the performance of such complementary pharmaceutical supply systems in low-income settings. The current study analyses how accountability mechanisms contributed to the performance of Jazia Prime Vendor System (Jazia PVS) in Tanzania. Methods The study analysed financial, performance and procedure accountability as defined in Boven's accountability framework. We conducted 30 in-depth interviews (IDIs), seven group discussions (GD) and 14 focus group discussions (FGDs) in 2018 in four districts that implemented Jazia PVS. We used a deductive and inductive approach to devel health facilities, including health facility governing committees, which are responsible for priority-setting and decision-making at facility level.Increasing conservation and animal-welfare concerns have driven the development of non-lethal sampling of fish populations, with the use of muscle tissue biopsies now being routinely applied as a sampling method in the wild. Crucial to the success of non-lethal sampling, however, is an evaluation of the short- and long-term consequences of the treatment and ultimately the determination of how these may affect organism mortality and other fitness-related traits. The current study evaluated the use of a dorsal muscle biopsies on post-spawned Atlantic salmon emigrating to sea and undertaking a 2-month long-feeding migration before returning to spawn. Using mark-recapture, return rates and growth were compared between fish that were biopsied and externally tagged, and a control group tagged only with external tags. [https://www.selleckchem.com/products/glutathione.html GSH concentration] The biopsy treatment showed no lasting effects on fish as estimated from the two key fitness-related parameters. Results, therefore, suggest the technique can be more widely applied to gather information on marine migrating Atlantic salmon and other anadromous fishes that can be intercepted as they descend and ascend rivers during seasonal migrations.