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The COVID-19 pandemic points to the need for scientists to pool their efforts in order to understand this disease and respond to the ensuing crisis. Other global challenges also require such scientific cooperation. Yet in academic institutions, reward structures and incentives are based on systems that primarily fuel the competition between (groups of) scientific researchers. Competition between individual researchers, research groups, research approaches, and scientific disciplines is seen as an important selection mechanism and driver of academic excellence. These expected benefits of competition have come to define the organizational culture in academia. There are clear indications that the overreliance on competitive models undermines cooperative exchanges that might lead to higher quality insights. This damages the well-being and productivity of individual researchers and impedes efforts towards collaborative knowledge generation. Insights from social and organizational psychology on the side effects of relying on performance targets, prioritizing the achievement of success over the avoidance of failure, and emphasizing self-interest and efficiency, clarify implicit mechanisms that may spoil valid attempts at transformation. The analysis presented here elucidates that a broader change in the academic culture is needed to truly benefit from current attempts to create more open and collaborative practices for cumulative knowledge generation.Heavy metal pollution not only decreases crop yield and quality, but also affects human health via the food chain. Ubiquitination-dependent protein degradation is involved in plant growth, development, and environmental interaction, but the functions of ubiquitin-ligase (E3) genes are largely unknown in tomato (Solanum lycopersicum L.). Here, we functionally characterized a RING E3 ligase gene, SlRING1, which positively regulates cadmium (Cd) tolerance in tomato plants. An in vitro ubiquitination experiment shows that SlRING1 has E3 ubiquitin ligase activity. The determination of the subcellular localization reveals that SlRING1 is localized at both the plasma membrane and the nucleus. Overexpression of SlRING1 in tomato increased the chlorophyll content, the net photosynthetic rate, and the maximal photochemical efficiency of photosystem II (Fv/Fm), but reduced the levels of reactive oxygen species and relative electrolyte leakage under Cd stress. Moreover, SlRING1 overexpression increased the transcript levels of CATALASE (CAT), DEHYDROASCORBATE REDUCTASE (DHAR), MONODEHYDROASCORBATE REDUCTASE (MDHAR), GLUTATHIONE (GSH1), and PHYTOCHELATIN SYNTHASE (PCS), which contribute to the antioxidant and detoxification system. Crucially, SlRING1 overexpression also reduced the concentrations of Cd in both shoots and roots. Thus, SlRING1-overexpression-induced enhanced tolerance to Cd is ascribed to reduced Cd accumulation and alleviated oxidative stress. Our findings suggest that SlRING1 is a positive regulator of Cd tolerance, which can be a potential breeding target for improving heavy metal tolerance in horticultural crops.Dental extractions are a common part of general dental practice. While dentists routinely screen for medical contraindications during the preoperative assessment, undiagnosed coagulopathies have the potential to severely complicate a seemingly routine extraction. We report a case of surgical removal of a mandibular third molar in a patient with undiagnosed Von Willebrand Disease.<br /><br /> Frozen embryo transfer is associated with a higher rate of live birth and a lower risk for ovarian hyperstimulation syndrome in women with polycystic ovary syndrome (PCOS) compared with fresh embryo transfer. The aim of this study is to assess the optimal endometrial preparation protocol for women with PCOS undergoing frozen embryo transfer.<br /><br /> We conducted a historical cohort analysis of 1720 women with PCOS who underwent the "freeze-all" strategy between August 2014 and August 2017 because of their high risk for ovarian hyperstimulation syndrome. Three endometrial preparation protocols were used natural cycle (NC; n=191), which relies on the dominant follicle to secrete estrogen that then promotes endometrial growth; ovarian stimulation (OS; n=96), which induces follicle growth using low doses of human menopausal gonadotropin; and hormone replacement (HRT; n=1433), which uses exogenous estradiol to promote endometrial growth. The primary outcome was live birth.<br /><br /> For women who received a single embryo transfer, the live birth rates for the NC, OS, and HRT groups were 62.4%, 65.0%, and 52.2%, respectively. The live birth rate in the HRT group was significantly lower than that seen in the OS and NC groups (P=.009). The clinical pregnancy rates of the three groups were 72.3%, 73.8%, and 64.9%, respectively; this difference did not reach statistical significance (P=.071).<br /><br /> The rate of live birth with the NC and OS regimens was higher than with the HRT protocol in women with PCOS who undergo single-blastocyst frozen embryo transfer.<br /><br />The rate of live birth with the NC and OS regimens was higher than with the HRT protocol in women with PCOS who undergo single-blastocyst frozen embryo transfer.Matrix metalloproteinases (MMPs) are zinc-dependent endo-peptidases that in mammals are known to be involved in remodeling the extracellular matrix (ECM) in developmental and pathological processes. In this study, we report At5-MMP of Arabidopsis thaliana to be important for root development and root bacterial communities. At5-MMP is mainly localized in the root vasculature and lateral root, an At5-MMP T-DNA insertion mutant (mmp5 KO) showed reduced root growth and a lower number of root apexes, causing reduced water uptake from the soil. Subsequently, mmp5 KO is sensitive to drought stress. Inhibited auxin transport was accompanied with resistance to indole-3-acetic acid (IAA), 2, 4-dichlorophenoxyacetic acid (2, 4-D), and 1-naphthaleneacetic acid (NAA). The content of endogenous abscisic acid (ABA) was lower in roots of mmp5 KO than in wild type. Genes responsive to ABA as well as genes encoding enzymes of the proline biosynthesis were expressed to a lower extent in mmp5 KO than in wild type. [https://www.selleckchem.com/products/phenazine-methosulfate.html selleck compound] Moreover, drought stress modulated root-associated bacterial communities of mmp5 KO the number of Actinobacteria increased. Therefore, At5-MMP modulates auxin/ABA signaling rendering the plant sensitive to drought stress and recruiting differential root bacterial communities.Restricted mean survival time (RMST) is a clinically interpretable and meaningful survival metric that has gained popularity in recent years. Several methods are available for regression modeling of RMST, most based on pseudo-observations or what is essentially an inverse-weighted complete-case analysis. No existing RMST regression method allows for the covariate effects to be expressed as functions over time. This is a considerable limitation, in light of the many hazard regression methods that do accommodate such effects. To address this void in the literature, we propose RMST methods that permit estimating time-varying effects. In particular, we propose an inference framework for directly modeling RMST as a continuous function of L. Large-sample properties are derived. Simulation studies are performed to evaluate the performance of the methods in finite sample sizes. The proposed framework is applied to kidney transplant data obtained from the Scientific Registry of Transplant Recipients.<br /><br /> Benign capsular nevi (BCN) are not infrequent in sentinel lymph nodes (SLN) of patients with melanoma. Their prognostic significance is unknown and the literature is limited. This study evaluated the clinical significance of incidentally found BCN in these patients.<br /><br /> A multi-institutional retrospective review of patients undergoing SLN biopsy for cutaneous melanoma between 2000 and 2016. Patients were divided into the following groups (a) negative SLN and no BCN, (b) negative SLN and presence of BCN, (c) positive SLN seen only on immunohistochemistry (IHC), and (d) positive SLN via hematoxylin and eosin (H&amp;E). Outcomes measured were overall survival and any recurrence.<br /><br /> A total of 1253 patients were identified (group 1 = 978, group 2 = 56, group 3 = 32, and group 4 = 187). Fifty-seven percent were male and the mean age was 59.3 years. link2 BCN was identified in 77 patients (6.2%), of which the majority was in the node-negative group (72%). Multivariable analysis showed that BCN was associated with lower recurrence rates, though not statistically significant (hazard ratio [HR] = 0.5; P = .06). IHC- and H&amp;E-positive SLNs were associated with a higher risk of recurrence (HR = 2.4; P = .02 and 2.0, P &lt; .0001, respectively).<br /><br /> Patients with BCN and negative SLN had lower recurrence rates than patients with negative SLN and no BCN. Our data suggest a possible protective effect against recurrence.<br /><br />Patients with BCN and negative SLN had lower recurrence rates than patients with negative SLN and no BCN. Our data suggest a possible protective effect against recurrence.<br /><br /> The myriad of coexisting risk factors for tinea capitis (TC) in parts of Africa makes it common among schoolchildren from this region. Diagnosis ideally involves microscopy and culture of scalp scrapings, which is not always feasible in resource-poor settings. Small-scale reports have identified trichoscopy (dermoscopy of hair and surrounding skin) as a cheap, quick, and simple tool for evaluating TC. We sought to describe the trichoscopic features of TC among Nigerian children.<br /><br /> A total of 204 children with clinically diagnosed TC had trichoscopy of their scalp lesions and culture of scalp scrapings. link3 Trichoscopic features and culture results were noted.<br /><br /> Perifollicular scaling (78.4%), interfollicular scaling (69.1%), and black dots (44.1%) were the commonest trichoscopic features. Others include regrowing hairs (18.6%), comma hairs (14.7%), broken hairs (13.2%), corkscrew hairs (3.4%), zigzag hairs (2.5%), and peripilar casts (2.0%). All children with comma, corkscrew, and zigzag hairs, as well as most with black dots (88.9%), broken hairs (88.9%), and regrowing hairs (77.5%) had positive cultures, irrespective of the isolated dermatophyte. Comma hairs were specific for Tinea infection with positive culture of dermatophytes. The presence of black dots in combination with individual features like broken hairs, regrowing hairs, perifollicular scaling, or interfollicular scaling also had a high specificity for positive cultures.<br /><br /> In the management of children with TC, trichoscopy is useful as a diagnostic tool, especially where access to laboratory evaluation is either not instant or unavailable.<br /><br />In the management of children with TC, trichoscopy is useful as a diagnostic tool, especially where access to laboratory evaluation is either not instant or unavailable.Actinidia arguta pollen owns abundant nutrients, such as vitamins, polyphenols, etc., however, little research on its antioxidant ability and biological function was conducted. In this study, we observed A. arguta pollen spore structure by SEM (Scanning electron microscope), analyzed the phenolic composition of A. arguta pollen extract (AAPE) obtained by four extraction methods (A ultrasound-assisted extraction with water, B heat reflux extraction with water, C ultrasound-assisted extraction with ethanol, and D heat reflux extraction with ethanol). Total phenolic content, total flavonoid content, antioxidant activities (ferric reducing/antioxidant power [FRAP], chelating activity, and DPPH⋅ scavenging activity) were also determined. Finally, we investigated its protective effect on DNA and lymphocytes damage response to oxidative stress. The results showed that the morphology of A. arguta pollen was similar to other pollen of the genus A. Lindl., but differs from them slightly in the specific morphology indicators.