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The mechanical disruption and removal of the subgingival biofilm represent the most important step in the treatment of periodontitis. However, in deep periodontal pockets, mechanical removal of the subgingival biofilm is difficult and frequently incomplete. Preliminary findings indicate that the use of amino acid buffered sodium hypochlorite (NaOCl) gel may chemically destroy the bacterial biofilm and facilitate its mechanical removal.<br /><br /> To clinically evaluate the efficacy of minimally invasive nonsurgical therapy (MINST) of periodontal pockets with or without local application of an amino acid buffered sodium hypochlorite (NaOCl) gel.<br /><br /> Forty untreated patients diagnosed with severe/advanced periodontitis (i.e. stage III/IV) with a slow/moderate rate of progression (i.e. grade A/B) were randomly allocated in two treatment groups. In the test group, the periodontal pockets were treated by means of MINST and NaOCl gel application, while in the control group, treatment consisted of MINST alone. Full-mouth plaate that (a) the use of MINST may represent a clinically valuable approach for nonsurgical therapy and (b) the application of NaOCl gel in conjunction with MINST may additionally improve the clinical outcomes compared to the use of MINST alone.<br /><br /> In patients with untreated periodontitis, treatment of deep pockets by means of MINST in conjunction with a NaOCl gel may represent a valuable approach to additionally improve the clinical outcomes obtained with MINST alone.<br /><br />In patients with untreated periodontitis, treatment of deep pockets by means of MINST in conjunction with a NaOCl gel may represent a valuable approach to additionally improve the clinical outcomes obtained with MINST alone.<br /><br /> Periodontitis may contribute to vascular damage, resulting in the destabilization of atherosclerotic plaque leading to acute coronary syndrome (ACS). In this study, we explored the effect of non-surgical periodontal treatment (NSPT) on cardiovascular blood biomarkers and gingival crevicular fluid (GCF) neutrophil elastase (NE) and α1-proteinase inhibitor (α-1PI) levels in periodontitis (P) participants with and without ACS.<br /><br /> Medical and dental examinations were performed to diagnose ACS and periodontitis, respectively. Seventeen patients with diagnosis of ACS and periodontitis were included in this study, as a test group (group ACS). Twenty-six age and sex-matched control patients with periodontitis (group P) were otherwise systemically healthy. Both groups received NSPT. Plasma levels of cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL), C-reactive protein (CRP), GCF NE activity, GCF α<br /><br /> -PI levels, and GCF NE/α1-PI rates were measured at baseline, at1<br /><br /> and 3<br /><br /> months after NSPT.<br /><br /> GCF NE activity/time (μU/30s) decreased significantly at 3<br /><br /> month compared to baseline values in the Group P after NSPT. First and 3<br /><br /> months after NSPT, in the Group P GCF α<br /><br /> -PI activity/time (pg/30s) was significantly higher than the Group ACS. Moreover GCF NE/α<br /><br /> -PI rates decreased significantly compared to baseline values at 1<br /><br /> and 3<br /><br /> months after NSPT in the group P.<br /><br /> NSPT yields decrease in NE/α<br /><br /> -PI rates. NE and its possible interactions with α<br /><br /> -PI may play a crucial role in both periodontitis and ACS. GCF α1PI activity/time (U/30s) can be a potential biomarker in management of periodontitis associated with ACS.<br /><br /> The GCF α1-PI reduction may alter the immune-inflammatory response in patients with periodontitis and thus increase the risk of ACS.<br /><br /> Thai Clinical Trials.gov (NCT04785235).<br /><br />Thai Clinical Trials.gov (NCT04785235).A reagent-less DNA sensor has been developed exploiting a combination of gold nanoparticles, modified primers, and isothermal amplification. It is applied to the determination ofKarlodinium armiger, a toxic microalgae, as a model analyte to demonstrate this generic platform. Colloidal gold nanoparticles with an average diameter of 14 ± 0.87 nm were modified with a mixed self-assembled monolayer of thiolated 33-mer DNA probes and (6-mercaptohexyl) ferrocene. Modified primers, exploiting a C3 spacer between the primer-binding site and an engineered single-stranded tail, were used in an isothermal recombinase polymerase amplification reaction to produce an amplicon by two single-stranded tails. These tails were designed to be complementary to a gold electrode tethered capture oligo probe, and an oligo probe immobilized on the gold nanoparticles, respectively. The time required for hybridization of the target tailed DNA with the surface immobilized probe and reporter probe immobilized on AuNPs was optimized and reduced to 10 min, in both cases. Amplification time was further optimized to be 40 min to ensure the maximum signal. Under optimal conditions, the limit of detection was found to be 1.6 fM of target dsDNA. Finally, the developed biosensor was successfully applied to the detection of genomic DNA extracted from a seawater sample that had been spiked with K. armiger cells. The demonstrated generic electrochemical genosensor can be exploited for the detection of any DNA sequence and ongoing work is moving towards an integrated system for use at the point-of-need.Pharmaceuticals such as oxytetracycline and paracetamol are extensive chemicals in the aquatic systems. In this study, the removal performance of oxytetracycline and paracetamol was investigated in the same enriched feed water medium by sequencing batch aerobic/anaerobic reactor system. [https://www.selleckchem.com/Proteasome.html Selleck Proteasome inhibitor] In this context, oxytetracycline and paracetamol in the aerobic phase were removed by a maximum of 66 and 99.8% respectively. At the same time, nitrification and denitrification removals were obtained as 95% and 98%, respectively. On the other hand, oxytetracycline and equivalent O2 flux of oxytetracycline maximum were calculated as 1.18 and 2.14 mg/L.d and the maximum removal volumetric flux of paracetamol and its O2 equivalent flux were determined approximately as 136 and 303 mg/L.d, simultaneously. In addition, oxytetracycline and paracetamol were given to the system in an amount of maximum 1 and 500 mg/L, respectively. Paracetamol has not significantly affected nitrification and denitrification up to 120 mg/L, but 500 mg/L paracetamol has completely finished denitrification in this system.