Zobrazení zdroje stránky Olivermcdonough5655

To fully exploit the potential of laboratory automation, future generations of scientists will require both engineering and biology skills. Automation in the research laboratory is likely to be an increasingly critical component of future research programs and will continue the trend of combining engineering and science expertise together to answer novel research questions.Infectious disease of poultry and pig are major threat to health and cause severe economic loss to the food industry and a global food safety issue. Poultry and pig act as a mixing vessel of zoonotic transmission of disease to humans. Effective mucosal vaccines used in animals could reduce the impact of diseases in food animals. Chitosan is a biocompatible polymer, and its positive charge makes it a natural mucoadhesive agent. Therefore, since last one-decade chitosan derived nanoparticles (CS NPs) have been in use widely to deliver vaccine antigens in animals through mucosal route. Primary route of entry of most infectious disease pathogen is through oral and nasal routes, and the CS NPs based vaccines delivered through that routes enhance the immunogenicity of encapsulated vaccine antigens by targeting the cargo to mucosal microfold cells, dendritic cells and macrophages. Resulting in induction of robust secretory and systemic antibodies and/or cell mediated immune response which provides protection against infections. To date, CS NPs is being widely used for mucosal vaccine delivery in poultry and pigs to control bacterial and viral infections, and tested in several preclinical trials for vaccine delivery in humans. In this review, we highlighted the progress so far made in using CS NPs as a vehicle for mucosal vaccine delivery against infectious and zoonotic diseases of poultry and pigs. Discussed about the need of CS NPs modifications, CS NPs based vaccines induced immune responses and its role in protection, and challenges in vaccination and future directions.Demonstration of receptor-mediated targeting of nanoparticles to specific organs and/or cell types is an integral aim in many bionanomedicine development projects. However, engagement of targeted receptors with ligands on nanocarriers, which is the cornerstone of the active targeting concept, is challenging to study under biologically relevant conditions and thus often stays overlooked. In this work, we utilize an in-house established bioassay for in vitro targetability validation of mesoporous silica nanoparticles (MSNs), functionalized with high-affinity peptide ligands to somatostatin receptors via protective group chemistry, ensuring the correct orientation of the peptide's pharmacophore. We demonstrate that targeted nanoparticles, but not scrambled peptide-decorated counterparts, specifically engage the targeted receptors in living cells in culture media containing serum protein. The importance of being able to exclude false positives originating from the premature detachment of targeting peptides from the MSNs is highlighted.Environmental pollution as a result of urban and industrial wastewater has become an increasingly prominent issue. Rivers, lakes, and oceans that have become eutrophicated or polluted by suspended solids and hazardous substances in wastewater have endangered the environment. A root cause of this is the discharge of untreated urban and industrial wastewater into the ecosystem. [https://www.selleckchem.com/products/ml349.html ML349] As a solution to the pollution, wastewater treatment facilities have seen increasingly rapid development. Sewage pumps are designed to transport urban and industrial wastewater containing solid particles or hazardous substances to water treatment centers for purification and treatment. Sewage pumps are of great importance in the entire wastewater treatment system. Sewage in the environment where sewage pumps work usually contains sands, suspended particles, and plenty of saline ions. Flow passage components and sealing elements of the pump become vulnerable to abrasion and chemical corrosion, which further challenges operational stability of the pump. Research has remained focused on how to improve reliability of sewage pumps under severe conditions. Because of advances in materials science, the application of an increasing number of new materials has been witnessed, such as carbon-based composite materials and carbon nanomaterials, thanks to their fine self-lubrication performance, heat resistance, thermal and electrical conductivity, chemical stability, heat and seismic resistance, as well as plasticity. These properties contribute to enormous potential that new carbon-based composite materials and carbon nanomaterials have to offer in terms of corrosion resistance. This paper outlines application scenarios, research progress, and the prospect of new materials in sewage pumps.Hierarchically nanoporous carbon materials (HNCMs) with well-defined morphology and excellent electrochemical properties are promising in fabrication of energy storage devices. In this work, we made a comparative study on the supercapacitive performances of HNCMs with different morphologies. To this end, four types of HNCMs with well-defined morphologies including submicrospheres (HNCMs-S), hexagonal nanoplates (HNCMs-N), dumbbell-like particles (HNCMs-D), and hexagonal microprisms (HNCMs-P) were successfully synthesized by dual-template strategy. The relationship of structural-electrochemical property was revealed by comparing the electrochemical performances of these HNCMs-based electrodes using a three-electrode system. The results demonstrated that the HNCMs-S-based electrode exhibited the highest specific capacitance of 233.8 F g-1 at the current density of 1 A g-1 due to the large surface area and well-defined hierarchically nanoporous structure. Moreover, the as-prepared HNCMs were further fabricated into symmetrical supercapacitor devices (HNCMs-X//HNCMs-X) using KOH as the electrolyte and their supercapacitive performances were checked. Notably, the assembled HNCMs-S//HNCMs-S symmetric supercapacitors displayed superior supercapacitive performances including high specific capacitance of 55.5 F g-1 at 0.5 A g-1, good rate capability (retained 71.9% even at 20 A g-1), high energy density of 7.7 Wh kg-1 at a power density of 250 W kg-1, and excellent cycle stability after 10,000 cycles at 1 A g-1. These results further revealed the promising prospects of the prepared HNCMs-S for high-performance energy storage devices.