Zobrazení zdroje stránky Mirandavognsen6547

Iron deficiency anemia (IDA) is a worldwide nutritional problem. The metabolic mechanism of IDA is still unclear. So, the underlying metabolic mechanism of iron supplementation has not been reported even if various iron supplements to treat IDA have been studied. The present study aimed to investigate the metabolic mechanisms of IDA and agar oligosaccharide-iron complex (AOS-iron) supplementation in IDA rats by assessing the changes of endogenous metabolites in serum and liver using LC-MS/MS metabolomics approach. Orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed significant separation of metabolites in serum and liver among the normal, anemia model and AOS-iron groups. Seventeen and eight metabolites were identified from serum and liver, respectively. Pathway enrichment analysis suggested that potential biomarkers were strongly involved in the biosynthesis of saturated and unsaturated fatty acids, sphingolipid metabolism, glycerophospholipid metabolism, linoleic acid metabolism, Fcγ receptor (FcγR)-mediated phagocytosis, pancreatic cancer metabolism, regulation of autophagy, gonadotropin releasing hormone (GnRH) signaling pathway, fatty acid metabolism, pantothenate and CoA biosynthesis, glutathione metabolism and primary bile acid biosynthesis. After supplementing 2&nbsp;mg Fe/kg·bw AOS-iron for 4&nbsp;weeks, the major metabolites in related pathways disrupted by IDA were restored to normal levels. Therefore, AOS-iron effectively treated IDA by regulating metabolic disorders. The regiodistribution of fatty acids (FAs) in triacylglycerols (TAGs) is highly correlated to the digestion and absorption of dietary fats. During in vitro gastric digestion, medium-long-medium/short chain triacylglycerols (MLM) showed the fastest digestibility among 105 TAG molecular species, for both raw and homogenised milk, resulting from the higher activity of gastric lipase towards medium-chain fatty acids (MCFAs) than towards long-chain fatty acids (LCFAs). After 60&nbsp;min of in vitro gastric digestion, the percentage of digestion of each MLM was 16.6-31.2% and 12.3-18.6% respectively in raw sheep and cow milk, and it reached 33.4-45.5% and 23.0-38.9% respectively, after homogenisation. Sheep milk TAGs were digested faster than cow milk TAGs in raw milk (16.1% and 12.4%, respectively) as well as after homogenisation (24.1% and 20.3%, respectively). This is due to the higher percentages of MCFAs esterified on the sn-1/3 positions of sheep milk TAGs (48.3-67.3% and 11.9-51.5% respectively for sheep and cow milk prior to and after homogenisation). Homogenisation favoured TAG lipolysis due to the increased fat globule surface area; however, it did not affect lipase regiospecificity towards TAG molecular species. High hydrostatic pressure (HHP) promotes the release of bioactive compounds from their intracellular compartments making them more bioaccessible. Our aim was to propose a schematic tissue model to explain the release mechanisms of betalains and phenolic compounds in vegetable cells submitted to HHP by analyzing cell microstructure, cell morphology, cell viability and the localization of bioactive compounds in prickly pear fruits. Prickly pear slices were pressurized at 100, 350 and 600&nbsp;MPa at 20&nbsp;°C. Chlorenchyma cells (in peels) and parenchyma cells (in pulps) were analyzed by transmission electron microscopy, confocal laser scanning microscopy and optical microscopy. After pressurization, the respiration and ethylene production of processed fruits were measured every 6&nbsp;h (during storage at 16&nbsp;°C and 75% RH for 24&nbsp;h). In chlorenchyma cells, HHP ruptured betalain-storing vesicles in the cytoplasm and possibly increased the activity of endogenous enzymes. Contrarily, HHP released betalains from the vacuoles of ms of bioactive compounds in vegetable cells subjected to HHP. The Fabaceae family of plants include a variety of seeds with multiple shapes, sizes, and colors; with a great diversity of bioactive compounds found in legume seeds. Legumes are an excellent source of protein, peptides and phytochemicals which are present in significant amounts. These bioactive compounds have been reported to reduce the risk of developing non-communicable diseases (NCD), such as obesity and type-2 diabetes. In this narrative review, we discuss the biological potential of bioactive compounds found in legumes and the health benefits associated with their consumption as an alternative approach in the management of NCD. Current extraction methods, characteristics of the bioactive compounds, and different in vitro and in vivo studies evaluating the bioactivity of legume bioactives are reviewed and discussed. The objective of this study was to propose a novel method to determine residues of the bio-insecticide spinetoram, which is a mixture of two components (spinetoram J and L), in honey from multifloral, rosemary and heather botanical origins; liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was the technique employed. An efficient sample treatment (recoveries between 82% and 95%) involving a solid-phase extraction with a polymeric sorbent has been recommended, and no matrix effect was observed. Chromatographic analysis (4&nbsp;min) was performed in reverse phase mode by using a fused-core column (Kinetex® EVO C18) with acetonitrile and ammonium formate as the mobile phase components, which was applied in isocratic elution mode. Method was validated according to the current European legislation. Not only was it selective, but it also displayed a wide linear range, good precision (relative standard deviation values lower than 9%) and sensitivity (low limits of detection (spinetoram J, 0.1-0.3&nbsp;μg/kg; spinetoram L, 0.1-0.2&nbsp;μg/kg) and quantification (spinetoram J, 0.3-1.2&nbsp;μg/kg; spinetoram L, 0.4-0.7&nbsp;μg/kg)). Several honey samples were analyzed with this method and no spinetoram residues were found above the limits of detection. Buriti and pequi oils are rich in carotenoids and beneficial to human health; however, carotenoid oxidation during storage causes color loss in foods, making it difficult to use these oils in food products. This research aimed to encapsulate pequi oil and co-encapsulate pequi and buriti oils by emulsification using whey protein isolate (WPI) as an emulsifier in two forms, natural (unheated) and heated, followed by freeze-drying. The emulsions were studied by droplet size under different stress conditions, instability index, and rheology. The freeze-dried (FD) samples were studied after accelerated oxidation and the total carotenoid retention was determined; for the reconstituted FD, the zeta potential and droplet size were recorded after storage at 37&nbsp;°C for 30&nbsp;days. The emulsions were stable in all conditions, with average droplet sizes between 0.88&nbsp;±&nbsp;0.03 and 2.33&nbsp;±&nbsp;0.02&nbsp;μm, and formulations with heated WPI presented the lowest instability index values. [https://www.selleckchem.com/products/hydroxychloroquine-sulfate.html Selleck Hydroxychloroquine] The FD's zeta potential values ranged from -50&nbsp;±&nbsp;3 to -32&nbsp;±&nbsp;3&nbsp;mV.