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Additionally, using an SEM-EDX (Scanning electron microscopy-energy dispersive X-ray analyzer), we discovered that inorganic particles that formed within the activated sludge of the A/O-MBR steadily accumulating on the membrane surface, resulted in an evenly distributed inorganic layer which could be observed along the cross-sections of the cake layer. OBJECTIVE To analyze the association between heart-focused anxiety, depressive symptoms, health behaviors and healthcare utilization in patients with coronary heart disease (CHD). METHODS N&nbsp;=&nbsp;1007 patients with CHD were recruited in hospital and followed for one year in a two-site cohort study. Heart focused anxiety (Cardiac Anxiety Questionnaire [CAQ] with the three subscales fear, attention, and avoidance), depressive symptoms (depression module from the Patient Health Questionnaire [PHQ-9]), health behaviors and healthcare utilization (smoking status, alcohol consumption, physical activity, outpatient physician/psychotherapist visits) were assessed six months after the initial hospitalization. Multiple regression models were used for statistical analysis. RESULTS About one third of the sample exhibited clinically significant CAQ scores. Higher CAQ-avoidance scores were associated with current smoking (OR&nbsp;=&nbsp;1.62; 95%CI 1.33-1.98), reduced alcohol intake (OR&nbsp;=&nbsp;0.83; 95%CI 0.71-0.98), non-participation in a coronary exercise group (OR&nbsp;=&nbsp;1.76; 95%CI 1.42-2.17), less regular physical activity (OR&nbsp;=&nbsp;2.69; 95%CI 2.32-3.12), and more frequent contact to general practitioners (GPs; b&nbsp;=&nbsp;0.07, SE 0.03). CAQ-attention was associated with non-smoking (OR&nbsp;=&nbsp;0.51; 95%CI 0.37-0.70), exercise group participation (OR&nbsp;=&nbsp;0.69; 95%CI 0.51-0.94), more frequent regular physical activity (OR&nbsp;=&nbsp;0.55; 95%CI 0.44-0.68), and more frequent contact to specialists for internal medicine (b&nbsp;=&nbsp;0.09, SE 0.04). CAQ-fear was not associated with any of the health behavior or healthcare use measures. Depressive symptoms were associated with reduced regular physical activity (OR&nbsp;=&nbsp;1.05; 95%CI 1.02-1.08) and increased contact to mental care specialists (b&nbsp;=&nbsp;0.03, SE 0.01) and GPs (b&nbsp;=&nbsp;0.02, SE 0.01). CONCLUSIONS Heart-focused anxiety and depressive symptoms may impede secondary prevention in patients with CHD and increase outpatient healthcare utilization. Elicited broccoli suspension-cultured cells (SCC) provide a useful system for obtaining bioactive compounds, including glucosinolates (GS) and phenolic compounds (PCs). In this work, coronatine (Cor) and methyl jasmonate (MJ) were used to increase the bioactive compound production in broccoli SCC. Although the use of Cor and MJ in secondary metabolite production has already been described, information concerning how elicitors affect cell metabolism is scarce. It has been suggested that Cor and MJ trigger defence reactions affecting the antioxidative metabolism. In the current study, the concentration of 0.5 μM Cor was the most effective treatment for increasing both the total antioxidant capacity (measured as ferulic acid equivalents) and glucosinolate content in broccoli SCC. The elicited broccoli SCC also showed higher polyphenol oxidase activity than the control cells. Elicitation altered the antioxidative metabolism of broccoli SCC, which displayed biochemical changes in antioxidant enzymes, a decrease in the glutathione redox state and an increase in lipid peroxidation levels. Furthermore, we studied the effect of elicitation on the protein profile and observed an induction of defence-related proteins. All of these findings suggest that elicitation not only increases bioactive compound production, but it also leads to mild oxidative stress in broccoli SCC that could be an important factor triggering the production of these compounds. Canine babesiosis is an important global tick-borne infectious disease of domestic dogs and wild canids. B. gibsoni and B. vogeli are the most widespread species mainly endemic in dog population in southern and eastern regions of China. In this study, 272 blood samples were collected from pet dogs in five districts of Shenzhen, China. Babesia DNA was detected in 30 samples with an overall prevalence of 11.0 % in pet dogs in Shenzhen. The difference in the positive rate between female and male pet dogs, among different breed, and among the five sampling sites was not significant, while the age and health status of pet dogs significantly influence the positive rate of Babesia spp. infection. Sequencing results showed the presence of only B. vogeli in all of the samples tested. Sequence comparison and phylogenetic analysis revealed that all 30 strains of Babesia identified in this study shared the highest identity with B. vogeli. In sum, all results of this study showed a considerable high infection rate of Babesia spp. in pet dogs, and B. vogeli was the only Babesia species infecting pet dogs in Shenzhen city. Such information is necessary to help to elucidate the epidemiology of canine babesiosis in Shenzhen, and it is necessary to actively monitor this disease in pet dogs. The increased use of sunscreens and other cosmetics containing UV filters causes human and environmental burden. Avobenzone is a widely used UV filter. In its pure form it is known to undergo several transformations including photo-isomerisation, photodegradation, and halogenation. [https://www.selleckchem.com/products/amg-perk-44.html click here] Over 60 disinfection by-products were identified as transformation products of avobenzone in different disinfection reactions of chlorination and bromination in fresh and seawater. Two occasional samples of swimming pool water demonstrated the presence of some of these by-products at noticeable levels as judged by GC-MS peak areas. Although the toxicity of the majority of these products remain unknown, chlorinated phenols and acetophenones are known to be rather toxic. Aquatic bromination of avobenzone resulted in the identification of 33 disinfection by-products (DBPs). Many of them contain bromine in the molecular structure. Addition of copper salt slightly decreases conversion rate simultaneously increasing the levels of major brominated products. Photostability of 3 commercial sunscreen products (solar protection factor 30) containing avobenzone was studied under different experimental conditions including UVA/UVB, UVC photostimulation and chlorination. The commercial sunscreen products have completely different enhancing and inhibitory effect on avobenzone degradation under UVC light. The complex composition of commercial products caused also a protective shield in case of chlorinated solutions of commercial formulations exposed to chlorine and UVA/UVB light at the same time.