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This screening strategy demonstrates the feasibility of replacing existing complex mass spectrometry-based screening strategy with simple optical analysis, providing a new idea for the development of simple accurate screening technologies for widespread organic pollutants including pesticides.Rhizosphere soil physiochemical properties, enzyme activities and rhizosphere associated microbial communities are of the central importance for modulating phytoremediation in heavy metal contaminated soil. In this study, the rhizosphere micro-ecological characteristics of phytoremediation in seven groups of contaminated soil with different heavy metal species and concentrations were examined. The results showed that heavy metal-enrichment inhibited plant growth, but enhanced both anions (Cr2O72-) and cations (Cd2+ and Pb2+) uptake with corresponding mean values ranging from 19.37 to 168.74 mg/kg in roots and 10.89-86.53 mg/kg in shoots. Trifolium repens L. planting was able to compensate the lost caused by the heavy metal on the soil organic matter, available N, available P, available K and enzyme activities as well. According to the cluster, some species like Lysobacter, Kaistobacter and Pontibacter, was significantly related to heavy metal accumulation while others such as Flavisolibacter, Adhaeribacter and Bacillus promoted plant growth. The importance of root-associated microbial community could relatively regulate plant growth and heavy metal uptake. Our study not only illustrated the correlation among rhizosphere micro-ecological parameters, and the possible mechanisms of phytoremediation regulation, but also provide clear strategy for improving the phytoremediation efficiency.Microplastic contamination is not only a pressing environmental concern in oceans, but also terrestrial ecosystems. However, little is known about its potential impacts on pollinators. Here, we reported the effects of 25 μm-diameter spherical polystyrene microplastics (PS-MPs) alone or in combination with the antibiotic tetracycline on honey bees (Apis mellifera L.) in the laboratory. We noticed that PS-MPs exposure for 14 d had sublethal effects, with low mortalities (up to 1.6 %) across three different treatments (0.5, 5, and 50 mg/L) and no changes to the body weight gains compared to the control bees. Nevertheless, PS-MPs exposure led to significant decreases in the α-diversity of bees' gut microbiota accompanied by changes to the core microbial population structure. Additionally, PS-MPs lead to alterations in the expression of antioxidative (Cat), detoxification (CypQ1 and GstS3), and immune system-related genes (Domeless, Hopscotch, and Symplekin) in guts. More interestingly, we observed that PS-MPs accumulated and degraded inside of the hindgut and interacted with gut bacteria. The depletion of the normal gut microbiota using tetracycline dramatically increased the lethality of microplastics. These results provide a resource for future research on microplastic-microbiome interactions in other insects and also shed light on understanding the potential effects of microplastics in terrestrial ecosystems.The disposal and utilization of antibiotic mycelial dreg (AMD), which has been identified as a hazardous waste in China, are a serious concern because of the residual antibiotic and huge annual output. Pyrolysis is a promising technology to treat AMD. However, the pyrolysis of AMD is not studied in an adequate degree, particularly no attention has been paid to the release and distribution of the phosphorus in AMD during pyrolysis. Therefore, the present work studied the pyrolysis of AMD more comprehensively. The influence of pyrolysis temperature on product yields and characteristics, together with the release and distribution of nitrogen and phosphorus, and the antibiotic residue in products, were investigated. The results suggested that residual antibiotic was eliminated after pyrolysis. Nitrogen was mainly contained in the biochar and liquid products, while phosphorus was mainly retained in the biochar. Liquid products were characterized by abundant oxygen and nitrogen-containing compounds, while biochar was featured of both abundant nitrogen and inorganic phosphate groups. Pyrolysis temperature showed a significant effect on product yields and characteristics, and a low pyrolysis temperature is recommended considering the recycling of nitrogen and phosphorus. [https://www.selleckchem.com/products/PLX-4032.html selleck chemical] The disposal of AMD through pyrolysis conforms to the principles of AMD disposal.Antibiotic resistant bacteria with diverse resistance phenotypes and genotypes are ubiquitous in the environments that have become a global health concern. The role of DNA methylation in the dissemination of antibiotic resistance among different environments is currently unclear. We recovered 646 Enterobacteriaceae (Eb) isolates from hospital, livestock manure, municipal wastewater-treatment plants, river sediment and soil for comprehensive analysis of resistance phenotypes, β-lactamase genes, integrons, integron-associated gene cassettes and the levels of DNA methylation. Antibiotic susceptibility testing revealed that approximately 87.31 % isolates were multidrug resistant Eb. The β-lactamase genes were positively detected in 473 isolates with greater diversity in human or animal sourced Eb, while its prevalence was found to be highest in the Eb isolates from the natural environments. Forty-three gene cassettes (28 different types mediated by intI1) were detected in 53 (19.63 %) isolates, with greater diversity in Eb isolates from hospital and livestock manure. The multiple antibiotic resistance index of single strain was positively correlated with the 5-methylcytosine and showed a negative correlation with 6-methylademine. We conclude that the development of antibiotic resistance could possibly be coupled with DNA methylation, which might enhance the antimicrobial resistance and survival capacity of Eb.The treatment of VOCs (volatile organic compounds) in waste streams is very important. Herein, we propose to use a network microporous polyimide (PI) membrane for the molecular sieving of nitrogen over VOC molecules to control their emission. 2,6,14-triaminotriptycene (Trip) was reacted with aromatic dianhydride monomers, such as 3,3',4,4'-benzophenone tetracarboxylic dianhydride (BTDA), to synthesize ultramicroporous polyimides, which readily form composite membranes via solution coating. The properties of the PIs were characterized by X-ray photoelectron spectroscopy (XPS), Brunner-Emmet-Teller (BET) analysis, etc., which validated the formation of a network structure and ultramicroporosity in these polyimides. Therefore, the outstanding separation performance for the separation of nitrogen over VOCs, such as cyclohexane, by molecular sieving was obtained by using these membranes; a rejection higher than 99 % was realized with a permeability of approximately 2000∼2600 Barrer under a temperature of 25 °C and feed concentration of 30,000 ± 2000 ppm.