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Moreover, transcriptomics and metabolomics data revealed that the long-term exposure to nTiO2 affected the synthesis of carbohydrates, proteins, and lipids. However, the transcriptomics results indicated that the genes involved in ribosome biogenesis in eukaryotes pathway and TGF-beta signaling pathway were upregulated, which could explain why the growth and reproduction of earthworms were apparently not affected by the nTiO2 exposure. The combination of transcriptomics and metabolomics reveals the global responses that cannot be observed by conventional toxicity endpoints, facilitating the assessment of long-term ecological effect of engineered nanoparticles in the environment. The light absorption of brown carbon (BrC) makes a significant contribution to aerosol light absorption (Abs) and affects the radiative forcing. In this study, we analyzed and evaluated the light absorption and radiative forcing of BrC samples collected from December 2016 to January 2017 in Chongqing and Chengdu in the Sichuan Basin of Southwest China. Based on a two-component model, we estimated that BrC light absorption at 405&nbsp;nm was 19.9&nbsp;±&nbsp;17.1&nbsp;Mm-1 and 19.2&nbsp;±&nbsp;12.3&nbsp;Mm-1 in Chongqing and Chengdu, contributing 19.0&nbsp;±&nbsp;5.0% and 17.8&nbsp;±&nbsp;3.7% to Abs respectively. Higher Abs405,BrC, MAE405,BrC, and AAE405-980 values were observed during the pollution period over the clean period in both cities. The major sources of BrC were biomass burning (BB) and secondary organic aerosol in Chongqing, and coal combustion (CC) and secondary organic aerosol in Chengdu. During the pollution period, aged BrC formed from anthropogenic precursors via its aqueous reactions with NH4+ and NOx had impacts on BrC absorption in both cities. BB led to higher Abs405,BrC, MAE405,BrC, and AAE405-980 values in Chongqing than Chengdu during the pollution period. The fractional contribution of radiation absorbed by BrC relative to BC in the wavelengths of 405-445&nbsp;nm was 60.2&nbsp;±&nbsp;17.0% and 64.2&nbsp;±&nbsp;11.6% in Chongqing and Chengdu, significantly higher than that in the range of 405-980&nbsp;nm (26.2&nbsp;±&nbsp;6.7% and 27.7&nbsp;±&nbsp;4.6% respectively) (p&nbsp; less then &nbsp;0.001). This study is useful for understanding the characterization, sources, and impacts of BrC in the Sichuan Basin. V.A multi-season field trial was carried out to investigate the effect of the amendment of biochar, lime, ash and washed biochar on the growth of maize. A degraded, strongly acidic Ultisol (pHKCl 3.60), with a relatively high exchangeable aluminium content (2.4&nbsp;cmolc/kg) and a low exchangeable calcium content (0.99&nbsp;cmolc/kg), was used. Soil was treated once at the beginning of the field trial and crop growth was monitored over seven planting seasons (PS). All treatments increased maize yield. The average increases were; seven times for biochar, five times for lime, five times for washed biochar and eight times for ash treatment, when compared to the control across all PS. The effect of biochar, lime and ash treatments on maize yield were sustained over the seven PS. Soil pHKCl was significantly increased (p&nbsp; less then &nbsp;0.05 level) following the addition of all of the amendment materials. All treatments significantly reduced the concentration of Al3+ when compared to the control (p&nbsp; less then &nbsp;0.05), with the lowest concentrations for the lime and ash treatments. The ash treatment also increased the concentration of macronutrients (K, P and Mg) to the greatest extent. Results showed that there was a clear liming effect at play. The better performance of biochar compared to lime, despite lime having the highest pH and the lowest Al3+ concentration, can be explained by the additional K, Mg and P the biochar adds to the soil. Results also showed a clear nutrient addition effect where ash added the most nutrients. Overall, this work supports the fact that small scale farmers in Indonesia should produce biochar from their waste agricultural materials. Doing so not only provides an increase in crop productivity, but also sequesters carbon resulting in the best overall environmental benefit. The Changjiang (Yangtze) River Estuary (CJE) is one of the largest and most intense seasonal hypoxic zones in the world. [https://www.selleckchem.com/products/7acc2.html click here] Here we examine the possibility that submarine groundwater discharge (SGD) may contribute to the summer hypoxia. Spatial distributions of bottom water 222Rn suggest a hotspot discharge area in the northern section of the CJE. SGD fluxes were estimated based on a 222Rn mass balance model and were found to range from 0.002&nbsp;±&nbsp;0.004 to 0.022&nbsp;±&nbsp;0.011&nbsp;m3/m2/day. Higher SGD fluxes were observed during summer hypoxia period. The well-developed overlap of the distribution patterns for SGD flux and dissolved oxygen (DO) implies that SGD could be an important contributor to summer hypoxia in the region off the CJE. We suggest that SGD contributes to the seasonal hypoxia either (1) directly via discharge of anoxic groundwaters together with reducing substances; and/or (2) indirectly by delivering excess nutrients that stimulate primary productivity with consequent consumption of DO during organic matter decomposition. Understanding the role played by autochthonous functional microbes involved in the biotransformation of pollutants would help optimize bioremediation performance at contaminated sites. However, our knowledge of the remediation potential of indigenous diazotrophs in contaminated soils remains inadequate. Using a microcosm experiment, soil nitrogen fixation activity was manipulated by molybdenum (Mo) and tungsten (W), and their effect on the removal of polycyclic aromatic hydrocarbons (PAHs) was determined in agricultural and industrial soils. Results showed that after 42&nbsp;days of incubation, PAH dissipation efficiency was significantly enhanced by 1.06-fold in 600&nbsp;μg&nbsp;kg-1 Mo-treated agricultural soil, compared with that in the control. For the industrial soil, 1200&nbsp;μg&nbsp;kg-1 Mo treatment significantly promoted PAH removal by 90.76% in 21&nbsp;days, whereas no significant change was observed between treatments and control at the end of the incubation period. W also exerted a similar effect on PAH dissipation. The activity and gene abundance of nitrogenase were also increased under Mo/W treatments in the two soils.