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PURPOSE Excessive joint line obliquity (JLO) after open-wedge high tibial osteotomy (OWHTO) induces detrimental stress on the articular cartilage. The purpose of this article is to assess the correlation between JLO and the clinical results after OWHTO. METHODS 68 patients were followed up for more than 1&nbsp;year. JLO was assessed using a long-leg standing anteroposterior radiograph. The knee osteoarthritis outcome score (KOOS) and KSS (Knee Society score) objective knee score were assessed as clinical scores. The Weight-bearing line ratio (WBLR), medial proximal tibial angle (MPTA), lateral distal femoral angle (LDFA), and joint line convergence angle (JLCA) were assessed as radiological parameters. The timed up-and-go (TUG) test and single-leg standing (SLS) test were performed, and the isometric muscle strength of the quadriceps and hamstrings was assessed to evaluate the knee function. The primary outcomes were the correlations between the JLO and the clinical score, radiological parameters and knee function after OWHTO. The secondary objective of this study was to detect the factor with the greatest influence on JLO. RESULTS There were significant correlations between the postoperative JLO and the KOOS in the subcategories of pain, activities of daily living (ADL), and sports and recreation (r&nbsp;=&nbsp;-0.311, -0.302, -0.282, p&nbsp;=&nbsp;0.011, 0.014, 0.022, respectively). However, the postoperative JLO was not significantly correlated with the KSS, knee function, or muscle strength. The preoperative LDFA and postoperative MPTA were factors influencing increased JLO after OWHTO. DISCUSSION There was no significant correlation between the JLO and the actual knee function. The preoperative LDFA and postoperative MPTA were factors that influenced the increase in JLO after OWHTO. © The Authors, published by EDP Sciences, 2020.in English, French Title Variation interannuelle des communautés de parasites métazoaires de la carangue à gros yeux Caranx sexfasciatus (Pisces, Carangidae). Abstract Les communautés de parasites de Caranx sexfasciatus ont été caractérisées et analysées afin de déterminer si elles connaissent des variations interannuelles dans la structure et/ou la composition des espèces. Au total, 422 C. sexfasciatus ont été collectés dans la baie d’Acapulco, au Mexique, entre mai 2016 et mars 2019. Trente-deux taxons de parasites métazoaires ont été identifiés&nbsp; cinq Monogenea, treize Digenea, un Acanthocephala, un Cestoda, trois Nematoda, sept Copepoda et deux Isopoda. Les monogènes étaient les espèces de parasites les plus fréquentes et les plus abondantes au cours de toutes les années d’échantillonnage. La richesse en espèces de parasites au niveau de la communauté des composants variait considérablement de 8 (mai 2016) à 25 (mars 2019) et était similaire aux rapports précédents pour d’autres espèces de Carangidae. Les communautés et infracommunautés de C. sexfasciatus étaient caractérisées par un faible nombre d’espèces de parasites, une faible diversité et la dominance d’une seule espèce (le monogène Neomicrocotyle pacifica). La structure des communautés de parasites et la composition des espèces variaient entre les années d’échantillonnage et les saisons climatiques. Les fluctuations saisonnières ou locales de certains facteurs environnementaux biotiques et abiotiques expliquent probablement ces variations.In this work, we used a whole-genome sequencing (WGS) approach to study the features of KPC-producing Klebsiella pneumoniae (KPC-Kp) spreading in a large Italian long-term acute-care rehabilitation facility (LTACRF), and to track the dynamics of dissemination within this setting. [https://www.selleckchem.com/ selleck] Thirty-eight, non-replicated, KPC-Kp isolates from colonized patients (either already colonized at admission or colonized during admission), collected during 2016, were subjected to antimicrobial-susceptibility testing and WGS. All isolates were resistant to β-lactams, with the exception of ceftazidime/avibactam (97.4 % susceptible). The second most effective agent was fosfomycin, followed by colistin, trimethoprim/sulfamethoxazole, gentamicin and amikacin (92.1, 86.8, 60.5, 44.7 and 50 % of susceptibility, respectively). A large proportion of isolates (n=18/38, 47.4%) belonged to clonal group (CG) 101, and most of them (n=15) to a new sequence type (ST) designated as ST2502. All the CG101 isolates had a capsule locus type KL17. The ST2502 harboured the genes encoding for the yersiniabactin siderophore and the ArmA methylase, conferring high-level resistance to aminoglycosides. The second most represented lineage of isolates (16/38, 42.1%) belonged to ST512 of CG258. Analysing WGS data, we were able to ascertain the common origin of some isolates imported from other hospitals, and to track several clusters of in-LTACRF cross-transmissions. The results revealed that, in peculiar epidemiological settings such as LTACRF, new KPC-Kp clones different from those prevailing in acute-care hospitals and associated with uncommon resistance and virulence determinants can successfully emerge and disseminate.Two-component regulatory systems represent the major paradigm for signal transduction in prokaryotes. The simplest systems are composed of a sensor kinase and a response regulator. The sensor is often a membrane protein that senses a change in environmental conditions and is autophosphorylated by ATP on a histidine residue. The phosphoryl group is transferred onto an aspartate of the response regulator, which activates the regulator and alters its output, usually resulting in a change in gene expression. In this review, we present a historical view of the archetype EnvZ/OmpR two-component signaling system, and then we provide a new view of signaling based on our recent experiments. EnvZ responds to cytoplasmic signals that arise from changes in the extracellular milieu, and OmpR acts canonically (requiring phosphorylation) to regulate the porin genes and noncanonically (without phosphorylation) to activate the acid stress response. Herein, we describe how insights gleaned from stimulus recognition and response in EnvZ are relevant to nearly all sensor kinases and response regulators.